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Journal of Clinical Microbiology, September 2009, p. 2794-2801, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.00360-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Unexpectedly High Proportion of Ancestral Manu Genotype Mycobacterium tuberculosis Strains Cultured from Tuberculosis Patients in Egypt {triangledown}

Zeinab H. Helal,1,2 Mohamed Seif El-Din Ashour,1 Somaia A. Eissa,3 Ghanem Abd-Elatef,4 Thierry Zozio,5 Sankhiros Babapoor,2 Nalin Rastogi,5* and Mazhar I. Khan2*

Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt,1 Molecular Biology Laboratory, Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, Connecticut 06269-3089,2 Center of Tuberculosis, Department of Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt,3 Department of Chest Diseases, Faculty of Medicine, Al-Azhar University, Cairo, Egypt,4 Unité de la Tuberculose & des Mycobactéries, Institut Pasteur de la Guadeloupe, Abymes, Guadeloupe, France5

Received 17 February 2009/ Returned for modification 2 June 2009/ Accepted 16 June 2009

Tuberculosis is one of the important public health problems in Egypt. However, limited information on the Mycobacterium tuberculosis genotypes circulating in Egypt is available. A total of 151 M. tuberculosis strains were characterized by spoligotyping. The results revealed that 74.8% of M. tuberculosis isolates grouped into 13 different clusters, while 25.2% had unique spoligotype patterns. Comparison with an international spoligotyping database (the SITVIT2 database) showed that types SIT53 (T1 variant) and SIT54 (Manu2 variant) were the most common types between cluster groups. In addition, new shared types SIT2977, SIT2978, and SIT2979 were observed. The results identified for the first time an unusually high proportion of ancestral Manu strains of M. tuberculosis from patients in Egypt. The percentage of the Manu clade in this study (27.15%) was significantly higher than its overall representation of 0.4% in the SITVIT2 database. We show that in Egypt tuberculosis is caused by a predominant M. tuberculosis genotype belonging to the ancestral Manu lineage which could be a missing link in the split between ancestral and modern tubercle bacilli during the evolution of M. tuberculosis.

* Corresponding author. Mailing address for Mazhar I. Khan: Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road, Storrs, CT 06269-3089. Phone: (860) 486-0228. Fax: (860) 486-2794. E-mail: mazhar.khan{at}uconn.edu. Mailing address for Nalin Rastogi: Unité de la Tuberculose & des Mycobactéries, Institut Pasteur de la Guadeloupe, BP484, F97183 Abymes, Guadeloupe, France. Phone: 590-590-893881. Fax: 590-590-893880. E-mail: nrastogi{at}pasteur-guadeloupe.fr

{triangledown} Published ahead of print on 24 June 2009.

Journal of Clinical Microbiology, September 2009, p. 2794-2801, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.00360-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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