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Journal of Clinical Microbiology, September 2009, p. 2981-2984, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.00321-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evaluation of a New Rapid Antigen Test Using Immunochromatography for Detection of Human Metapneumovirus in Comparison with Real-Time PCR Assay{triangledown}

Yoko Matsuzaki,1* Emi Takashita,2 Michiko Okamoto,3 Katsumi Mizuta,4 Tsutomu Itagaki,5 Fumio Katsushima,6 Yuriko Katsushima,6 Yukio Nagai,7 and Hidekazu Nishimura3

Course of Clinical Nursing, Yamagata University Faculty of Medicine, Yamagata 990-9585, Japan,1 Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata 990-9585, Japan,2 Virus Research Center, Clinical Research Division, Sendai Medical Center, Sendai 983-8520, Japan,3 Yamagata Prefectural Institute of Public Health, Yamagata 990-0031, Japan,4 Yamanobe Pediatric Clinic, Yamagata 990-0301, Japan,5 Katsushima Pediatric Clinic, Yamagata 990-2461, Japan,6 Nagai Children's Clinic, Sendai 983-0045, Japan7

Received 13 February 2009/ Returned for modification 25 March 2009/ Accepted 22 June 2009

A new rapid human metapneumovirus (hMPV) detection kit using immunochromatography (SAS hMPV test) was compared to real-time PCR for 224 nasal swab specimens, 96.4% of which were obtained from children of <15 years of age. The overall sensitivity and specificity were 82.3% and 93.8%, respectively, suggesting that this test is useful for pediatricians to diagnose hMPV infection in a clinical setting.

* Corresponding author. Mailing address: Course of Clinical Nursing, Yamagata University Faculty of Medicine, Iida-Nishi 2-2-2, Yamagata 990-9585, Japan. Phone: 81-23-628-5249. Fax: 81-23-628-5250. E-mail: matuzaki{at}med.id.yamagata-u.ac.jp

{triangledown} Published ahead of print on 1 July 2009.

Journal of Clinical Microbiology, September 2009, p. 2981-2984, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.00321-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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