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Journal of Clinical Microbiology, September 2009, p. 2985-2988, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.00850-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Rapid and Highly Sensitive Neuraminidase Subtyping of Avian Influenza Viruses by Use of a Diagnostic DNA Microarray {triangledown} ,{dagger}

Astrid Gall,1 Bernd Hoffmann,1 Timm Harder,1 Christian Grund,1 Ralf Ehricht,2 and Martin Beer1*

Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-Insel Riems, Germany,1 Clondiag GmbH, Löbstedter Strasse 103-105, 07749 Jena, Germany2

Received 28 April 2009/ Returned for modification 10 June 2009/ Accepted 1 July 2009

Rapid neuraminidase subtyping of avian influenza viruses from diagnostic samples is crucial considering the existence of permanently emerging and evolving strains. Here we report an easy-to-use, low-cost microarray for neuraminidase subtyping following fragment amplification by a generic, neuraminidase-specific reverse transcription-PCR (RT-PCR). This method enables highly specific characterization with a sensitivity equal to that of matrix gene-specific real-time RT-PCR.

* Corresponding author. Mailing address: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-Insel Riems, Germany. Phone: 49 (0) 38351 7200. Fax: 49 (0) 38351 7151. E-mail: martin.beer{at}fli.bund.de

{triangledown} Published ahead of print on 8 July 2009.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, September 2009, p. 2985-2988, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.00850-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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