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Journal of Clinical Microbiology, September 2009, p. 2989-2991, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.02453-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Detection of Low Levels of Human Immunodeficiency Virus (HIV) May Be Critical for Early Diagnosis of Pediatric HIV Infection by Use of Dried Blood Spots{triangledown}

Jan Walter,1,{dagger} Louise Kuhn,2 Katherine Semrau,3 Don W. Decker,1 Moses Sinkala,4,§ Chipepo Kankasa,5 Donald M. Thea,3 Marc Bulterys,6,{ddagger} Chin-Yih Ou,7,{ddagger} and Grace M. Aldrovandi1*

Childrens Hospital Los Angeles, University of Southern California, Los Angeles, California,1 Gertrude H. Sergievsky Center and Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York,2 Center for International Health and Development at the Boston University School of Public Health, Boston, Massachusetts,3 Lusaka District Health Management Team, Lusaka, Zambia,4 University Teaching Hospital, University of Zambia, Lusaka, Zambia,5 Global AIDS Program, U.S. Centers for Disease Control and Prevention, Lusaka, Zambia,6 Global AIDS Program, U.S. Centers for Disease Control and Prevention, Atlanta, Georgia7

Received 21 December 2008/ Returned for modification 20 April 2009/ Accepted 14 July 2009

We compared a DNA-based assay with a total nucleic acid-based assay for early detection of infant human immunodeficiency virus (HIV) infection. The codetection of DNA and RNA did not result in an overall higher sensitivity compared to that of DNA alone. Discordant results were associated with low levels of HIV DNA, indicating that the sample amount may be critical.

* Corresponding author. Mailing address: Childrens Hospital Los Angeles, 4546 Sunset Boulevard (Mailstop 51), Los Angeles, CA 90027. Phone: (323) 361-8501. Fax: (323) 361-8599. E-mail: galdrovandi{at}chla.usc.edu

{triangledown} Published ahead of print on 22 July 2009.

{dagger} Present address: Department of Plant Pathology and Microbiology, University of California, Riverside, CA.

§ Present address: Catholic Medical Mission Board, Lusaka, Zambia.

{ddagger} Present address: CDC Global AIDS Program, Beijing, China.

Journal of Clinical Microbiology, September 2009, p. 2989-2991, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.02453-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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