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Journal of Clinical Microbiology, September 2009, p. 2992-2994, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.00599-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Real-Time Quantitative Reverse Transcription-PCR Assays Specifically Detecting Bluetongue Virus Serotypes 1, 6, and 8{triangledown} ,{dagger}

Bernd Hoffmann, Michael Eschbaumer, and Martin Beer*

Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald-Insel Riems, Germany

Received 24 March 2009/ Returned for modification 19 June 2009/ Accepted 8 July 2009

Bluetongue virus (BTV) is a major pathogen of ruminants. Especially serotypes 1, 6, and 8 are of concern to veterinary authorities in central Europe. This article describes highly sensitive real-time reverse transcription-PCR assays directed to BTV genome segment 2 for specific detection of BTV-1, -6, or -8 in animal samples.

* Corresponding author. Mailing address: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald-Insel Riems, Germany. Phone: 49-38351-7-200. Fax: 49-38351-7-226. E-mail: martin.beer{at}fli.bund.de

{triangledown} Published ahead of print on 15 July 2009.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, September 2009, p. 2992-2994, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.00599-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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