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Journal of Clinical Microbiology, September 2009, p. 3008-3010, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.01238-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Prevalence and Management of Invalid GeneXpert Enterovirus Results Obtained with Cerebrospinal Fluid Samples: a 2-Year Study{triangledown}

Susan E. Sefers,1 Anna K. Raymer,1 Jessica T. Kilby,1 David H. Persing,3,4 and Yi-Wei Tang1,2*

Departments of Pathology,1 Medicine, Vanderbilt University Medical Center, Nashville, Tennessee,2 Cepheid, Sunnyvale, California,3 Department of Pathology, Stanford University School of Medicine, Palo Alto, California4

Received 24 June 2009/ Accepted 6 July 2009

A total of 525 cerebrospinal fluid (CSF) samples submitted during the 2007 and 2008 enteroviral seasons were included in a study to determine the prevalence of and potential risk factors for invalid Cepheid GeneXpert enterovirus assay (GXEA) results, as well as possible solutions for the problem. The invalid GXEA results were reported for 43 (8.2%) specimens and correlated with increased visibility of red blood cells (P < 0.0001) but not with CSF xanthochromia and clotting. Invalid GXEA result rates were markedly diminished by 82.1% and 96.0% and test sensitivities were minimally decreased by 1.7% and 3.6% when these specimens were tested at a 1:5 dilution and after a freeze-thaw cycle, respectively.

* Corresponding author. Mailing address: Molecular Infectious Diseases Laboratory, Vanderbilt University Hospital, 4605 TVC, Nashville, TN 37232-5310. Phone: (615) 322-2035. Fax: (615) 343-8420. E-mail: yiwei.tang{at}vanderbilt.edu

{triangledown} Published ahead of print on 15 July 2009.

Journal of Clinical Microbiology, September 2009, p. 3008-3010, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.01238-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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