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Journal of Clinical Microbiology, September 2009, p. 3014-3016, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.01024-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Performance of the RealStar Chikungunya Virus Real-Time Reverse Transcription-PCR Kit{triangledown}

Marcus Panning,1,{dagger} Markus Hess,2 Waldemar Fischer,2 Klaus Grywna,1 Martin Pfeffer,3,{ddagger} and Christian Drosten1*

Institute of Virology, University Medical Centre, Bonn, Germany,1 astra Diagnostics, Hamburg, Germany,2 Bundeswehr Institute of Microbiology, Munich, Germany3

Received 23 May 2009/ Accepted 15 July 2009

A novel commercial Chikungunya virus real-time reverse transcription-PCR (RT-PCR) kit was evaluated on a comprehensive panel of original patient samples. The assay was 100% sensitive and specific in comparison to a published real-time RT-PCR. Viral loads from both assays were highly correlated. The kit proved to be suitable for routine use in patient care.

* Corresponding author. Mailing address: Institute of Virology, University of Bonn Medical Centre, Sigmund Freud-Str. 25, 53127 Bonn, Germany. Phone: 49-228-287-11055. Fax: 49-228-287-19144. E-mail: drosten{at}virology-bonn.de

{triangledown} Published ahead of print on 22 July 2009.

{dagger} Present address: Department of Virology, Freiburg University Medical Center, Freiburg, Germany.

{ddagger} Present address: Institute for Animal Hygiene and Veterinary Public Health, Leipzig, Germany.

Journal of Clinical Microbiology, September 2009, p. 3014-3016, Vol. 47, No. 9
0095-1137/09/$08.00+0     doi:10.1128/JCM.01024-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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