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J Clin Microbiol. 1977 May; 5(5): 531-534

Rapid biochemical tests for characterization of the Mycoplasmatales.

ABSTRACT

Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days.

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