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J Clin Microbiol. 1977 November; 6(5): 538-542

Modified Lombard-Dowell broth as a general growth medium.

ABSTRACT

A new liquid medium (modified Lombard-Dowell broth) was inoculated with stock culture strains of aerobic and anaerobic bacteria and compared with prereduced chopped-meat glucose inoculated with the same anaerobes. Both broths were subcultured at 48 and 72 h to aerobic and anaerobic blood agar plates, and the numbers of colonies were compared after 48-h incubation of the agar plates. This was repeated with mixed cultures of both aerobes and anaerobes. For a period of 11 months all specimens received for anaerobic-aerobic culture were inoculated into prereduced chopped-meat glucose and modified Lombard-Dowell broth plus the appropriate plate medium. Growth from subcultures was compared with primary plate isolates. Chopped-meat glucose and modified Lombard-Dowell broth isolates agreed, with the exception of one, Fusobacterium necrophorum, that did not grow in chopped-meat glucose.