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J Clin Microbiol. 1979 February; 9(2): 284-287

Complement-fixing immunoglobulin M antibody response in patients with infantile gastroenteritis.

Y Abe and S Inouye

ABSTRACT

Complement-fixing immunoglobulin M antibody to infantile gastroenteritis virus (a rotavirus) was detected with highest sensitivity when the antibody-antigen-complement mixture was incubated at 37 degrees C for 1 h prior to the addition of sensitized sheep erythrocytes. Sucrose gradient centrifugation of sera collected sequentially from four patients after infection detected 19S complement-fixing antibody up to 5 weeks, with highest titers at 1 week, after the onset of illness. Treatment of the whole sera with 2-mercaptoethanol decreased complement-fixing titers only up to 2 weeks after onset of illness.


J Clin Microbiol. 1979 February; 9(2): 284-287







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Copyright © 1979 by the American Society for Microbiology. All rights reserved.