Previous Article | Next Article 
J Clin Microbiol. 1979 May; 9(5): 584-588
Practical method to facilitate estimation of Streptococcus mutans levels in saliva.
B Köhler and
D Bratthall
ABSTRACT
A method was developed to facilitate the estimation of Streptococcus mutans levels in saliva. Saliva-contaminated wodden spatulas were pressed directly against an elevated agar plate containing a selective medium. The results were compared with the number of S. mutans per 1 ml of paraffin-stimulated saliva. It was shown that the spatula method gave a good estimation of the level of S. mutans infection. The incubation was also made in expired air instead of 95% N2-5% CO2. The outgrowth was in good agreement with that after conventional incubation. The method is useful in epidemiological studies or in selecting persons at a high caries risk, and when ordinary saliva sampling cannot be done, for example in small children. Compared with conventional saliva sampling, this method requires less time and material at sampling as well as at the laboratory.
J Clin Microbiol. 1979 May; 9(5): 584-588
This article has been cited by other articles:
-
Nogueira, R. D., Alves, A. C., Napimoga, M. H., Smith, D. J., Mattos-Graner, R. O.
(2005). Characterization of Salivary Immunoglobulin A Responses in Children Heavily Exposed to the Oral Bacterium Streptococcus mutans: Influence of Specific Antigen Recognition in Infection. Infect. Immun.
73: 5675-5684
[Abstract]
[Full Text]
-
Mattos-Graner, R. O., Li, Y., Caufield, P. W., Duncan, M., Smith, D. J.
(2001). Genotypic Diversity of Mutans Streptococci in Brazilian Nursery Children Suggests Horizontal Transmission. J. Clin. Microbiol.
39: 2313-2316
[Abstract]
[Full Text]
-
Gabre, P., Wikstrom, M., Martinsson, T., Gahnberg, L.
(2001). Move of Adults with Mental Retardation from Institutions to Community-based Living: Changes in the Oral Microbiological Flora. JDR
80: 421-426
[Abstract]
-
Mattos-Graner, R.O., Smith, D.J., King, W.F., Mayer, M.P.A.
(2000). Water-insoluble Glucan Synthesis by Mutans Streptococcal Strains Correlates with Caries Incidence in 12- to 30-month-old Children. JDR
79: 1371-1377
[Abstract]
-
Bratthall, D.
(1997). A Streptococcus mutans Safari!. JDR
76: 1332-1336
-
van Palenstein Helderman, W.H., Matee, M.I.N., van der Hoeven, J.S., Mikx, F.H.M.
(1996). Cariogenicity Depends More on Diet than the Prevailing Mutans Streptococcal Species. JDR
75: 535-545
[Abstract]
-
Van Houte, J.
(1993). Microbiological Predictors of Caries Risk. ADR
7: 87-96
[Abstract]
-
Sandham, H.J., Brown, J., Chan, K.H., Phillips, H.I., Burgess, R.C., Stokl, A.J.
(1991). Clinical Trial in Adults of an Antimicrobial Varnish for Reducing Mutans Streptococci. JDR
70: 1401-1408
[Abstract]
-
Salonen, L., Allander, L., Bratthall, D., Hellden, L.
(1990). Mutans Streptococci, Oral Hygiene, and Caries in an Adult Swedish Population. JDR
69: 1469-1475
[Abstract]
-
Jensen, B., Bratthall, D.
(1989). A New Method for the Estimation of Mutans Streptococci in Human Saliva. JDR
68: 468-471
[Abstract]
-
Axelsson, P., Kristoffersson, K., Karlsson, R., Bratthall, D.
(1987). A 30-month Longitudinal Study of the Effects of Some Oral Hygiene Measures on Streptococcus mutans and Approximal Dental Caries. JDR
66: 761-765
[Abstract]
-
Jordan, H.V., Laraway, R., Snirch, R., Marmel, M.
(1987). A Simplified Diagnostic System for Cultural Detection and Enumeration of Streptococcus mutans. JDR
66: 57-61
[Abstract]