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JCM Accepts, published online ahead of print on 7 May 2008
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J. Clin. Microbiol. doi:10.1128/JCM.00024-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Quadriplex PCR for simultaneous detection of serotype (O1 and/or O139), biotype, toxigenic potential and Central regulating factor of V. cholerae

Hemant Kumar Khuntia, Bibhuti Bhusan Pal, and Guru Prasada Chhotray*

Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar, Pin – 751023, Orissa, India

* To whom correspondence should be addressed. Email: rmrcpath{at}yahoo.co.in.


  Abstract

A Quadriplex PCR was developed for simultaneous detection of genes specific for V. cholerae O1 and/or O139 serogroup (wbe and/or wbf), cholera toxin A-subunit (ctxA), toxin co-regulated pilus (tcpA) and central regulating protein ToxR (toxR) in a single tube reaction. This is a simple and rapid accurate approach for detection of toxigenic V. cholerae O1 and/or O139 and can prevent rapid and wide spread of the disease by early detection




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