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JCM Accepts, published online ahead of print on 30 April 2008
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J. Clin. Microbiol. doi:10.1128/JCM.00230-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Optimization of the Roche PCR Analyte Specific Reagent Assay for Detection of Staphylococcus aureus, Methicillin-Resistant S. aureus (MRSA) and Vancomycin-Resistant Enterococcus species

Maitry S. Mehta, Suzanne M. Paule, Donna M. Hacek, Richard B. Thomson, Karen L. Kaul, and Lance R. Peterson*

Evanston Northwestern Healthcare, Evanston, IL; and Northwestern University's, Feinberg School of Medicine, Chicago, IL

* To whom correspondence should be addressed. Email: lancer{at}northwestern.edu.


  Abstract

Nasal and perianal swab specimens were tested for detection of Staphylococcus aureus and vancomycin-resistant Enterococcus species (VRE) using real-time PCR (Roche, Inc. analyte specific reagents) and microbiological cultures. Real-time PCR and culture for S. aureus were similar. PCR had adequate sensitivity but culture more specific for detection of VRE.




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