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JCM Accepts, published online ahead of print on 7 May 2008
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J. Clin. Microbiol. doi:10.1128/JCM.00315-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Comparsion of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for diagnosis of RNA and DNA respiratory viruses on nasopharyngeal aspirates

Kwok Hung Chan, Wing Cheong Yam, Chiu Mei Pang, Kit Man Chan, Siu Yan Lam, Kam Fai Lo, Leo L.M. Poon, and Malik J.S. Peiris*

Department of Microbiology, The University of Hong Kong and Queen Mary Hospital, Hong Kong, SAR, People's Republic of China; HKU-Pasteur Research Centre, Sassoon Rd, Pokfualm, Hong Kong SAR, People's Republic of China

* To whom correspondence should be addressed. Email: malik{at}hkucc.hku.hk.


  Abstract

The automated nucleic acid extraction systems NucliSens easyMAG and BioRot 9604 were evaluated in comparison with the manual QIAamp (QIAgen) extraction method for extracting nucleic acid from nasopharyngeal aspirates (NPA) for diagnosis of RNA and DNA respiratory viruses. Nucleic acid from all three methods gave comparable sensitivity in PCR tests and gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination.




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