JCM Accepts, published online ahead of print on 1 July 2009
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J. Clin. Microbiol. doi:10.1128/JCM.00321-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Evaluation of a new rapid antigen test using immunochromatography for the detection of human metapneumovirus compared with real-time PCR assay

Yoko Matsuzaki*, Emi Takashita, Michiko Okamoto, Katsumi Mizuta, Tsutomu Itagaki, Fumio Katsushima, Yuriko Katsushima, Yukio Nagai, and Hidekazu Nishimura

Course of Clinical Nursing, Yamagata University Faculty of Medicine, Yamagata 990-9585, Japan; Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata 990-9585, Japan; Virus Research Center, Clinical Research Division, Sendai Medical Center, Sendai 983-8520, Japan; Yamagata Prefectural Institute of Public Health, Yamagata 990-0031, Japan; Yamanobe Pediatric Clinic, Yamagata 990-0301, Japan; Katsushima Pediatric Clinic, Yamagata 990-2461, Japan; Nagai Children's Clinic, Sendai 983-0045, Japan

* To whom correspondence should be addressed. Email: matuzaki{at}med.id.yamagata-u.ac.jp.


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Abstract

A new rapid human metapneumovirus (hMPV) detection kit using immunochromatography (SAS hMPV test) was compared to real-time PCR for 224 nasal swab specimens, 96.4% of which were obtained from children <15 years of age. The overall sensitivity and specificity were 82.3% and 93.8%, respectively, suggesting that this test is useful for pediatricians to diagnose hMPV infection in a clinical setting.






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