JCM Accepts, published online ahead of print on 1 July 2009

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J. Clin. Microbiol. doi:10.1128/JCM.00367-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Rapid and Simple Determination of Ciprofloxacin Resistance in Clinical Strains of Escherichia coli

Rebeca Santiso, María Tamayo, José Luis Fernández^*, M del Carmen Fernández^*, Francisca Molina, Rosa Villanueva, Jaime Gosálvez, and Germán Bou

INIBIC-Complejo Hospitalario Universitario Juan Canalejo, Unidad de Genética, As Xubias 84, 15006- A Coruña, Spain; Laboratorio de Genética Molecular y Radiobiología, Centro Oncológico de Galicia, Avda. de Montserrat s/n, 15009-A Coruña, Spain; INIBIC-Complejo Hospitalario Universitario Juan Canalejo, Servicio de Microbiología, As Xubias, 84, 15006-A Coruña, Spain; Unidad de Genética, Facultad de Biología, Universidad Autónoma de Madrid, 28049-Madrid, Spain

^* To whom correspondence should be addressed. Email: JLFernandez{at}canalejo.org; joseluis.fernandez@cog.es.

We recently reported a simple new in situ diffusion assay, developed as a kit, to visualize DNA fragmentation in single bacterial cells. Use of this assay in a collection of 95 genetically-unrelated Escherichia coli clinical strains resulted in correct identification of all of the isolates as resistant or susceptible to ciprofloxacin, consistent with the MIC results. This relevant information is obtained in 80 minutes.