JCM Antimicrobial Agents and Chemotherapy
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JCM Accepts, published online ahead of print on 16 April 2008
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J. Clin. Microbiol. doi:10.1128/JCM.02179-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Evaluation of a new commercial real-time PCR quantification assay for prenatal diagnosis of cytomegalovirus congenital infection

Aurélie Ducroux, Samira Cherid, Alexandra Benachi, Yves Ville, and Marianne Leruez-Ville*

Laboratoire de Virologie. Centre Nationale de Référence du cytomégalovirus. Hôpital Necker-.Enfants-malades, Assistance Publique-Hôpitaux de Paris - Université Paris-Descartes-EA 36-20, 149 rue de Sèvres; 75015 Paris- France; Service de Gynécologie-Obstétrique, Assistance Publique-Hôpitaux de Paris, Hôpital Necker-.Enfants-malades, Assistance Publique-Hôpitaux de Paris - Université Paris-Descartes, 149 rue de Sèvres; 75015 Paris- France; Service de Gynécologie-Obstétrique, Hôpital de Poissy-Saint Germain en Laye, Université Saint-Quentin en Yvelines, 10 rue du Champ Gaillard 78000 Poissy -France

* To whom correspondence should be addressed. Email: marianne.leruez{at}nck.aphp.fr.


  Abstract

A new commercial real time HCMV PCR kit was evaluated after automated DNA extraction of 153 amniotic fluids in parallel with an in house real time PCR assay. The commercial kit displayed 100% sensitivity/specificity compared to the "in house" assay and was suitable for prenatal diagnosis of HCMV congenital infection.




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