JCM Accepts, published online ahead of print on 24 June 2009

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J. Clin. Microbiol. doi:10.1128/JCM.02182-08
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Proteomic identification of OprL as a seromarker for the initial diagnosis of Pseudomonas aeruginosa infection of patients with cystic fibrosis

Aparna R. Rao, Anita Laxova, Philip M. Farrell, and Joseph T. Barbieri^*

Department of Pediatrics and Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI and Department of Pediatrics, University of Wisconsin Medical School, Madison WI

^* To whom correspondence should be addressed. Email: jtb01{at}mcw.edu.

Identification of new immunogenic antigens that diagnose the initial Pseudomonas aeruginosa (PA) infection in patients with cystic fibrosis (CF) alone or as an adjunct to microbiology is needed. In the current study, a proteomic analysis was performed to obtain a global assessment of the host immune response during initial PA infection of patients with CF. MALDI-Mass Spectroscopy was used to identify outer membrane protein L (OprL), a non type III secretion system (TTSS) protein as an early immunogenic protein during the initial PA infection of patients with CF. Longitudinal Western blot analysis of sera from 12 of 14 patients with CF detected antibodies to OprL during the initial PA infection. In addition, antibodies were also detected to ExoS or ExoU or ExoS and ExoU, the latter implicating sequential PA infections during initial infections. Detection of serum reactivity to OprL, along with proteins of the TTSS, and in conjunction with microbiology may diagnose initial PA infections in patients with cystic fibrosis.