JCM Accepts, published online ahead of print on 5 March 2008
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J. Clin. Microbiol. doi:10.1128/JCM.02426-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Realtime RT-PCR Assay Unable to Detect H7 Subtype Avian Influenza Viruses Isolated from Wild Birds

Zheng Xing*, Carol Cardona, Phuong Dao, Beate Crossley, Sharon Hietala, and Walter Boyce

Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; California Animal Health and Food Safety Laboratory; Wildlife Health Center

* To whom correspondence should be addressed. Email: zxing{at}ucdavis.edu.


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Abstract

We report a failure of the current real-time RT-PCR H7 subtyping protocol, used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, designed originally for detecting H7 influenza viruses in poultry, is not reliable for wild bird surveillance.




This article has been cited by other articles:

  • Cardona, C., Slemons, R., Perez, D. (2009). The prevention and control of avian influenza: The avian influenza coordinated agriculture project. Poult. Sci. 88: 837-841 [Abstract] [Full Text]  


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