JCM Accepts, published online ahead of print on 24 June 2009

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J. Clin. Microbiol. doi:10.1128/JCM.02436-08
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Evaluation of MeningoFinder: a Novel MLPA Assay for Simultaneous Detection of Six Virus Species Causing Central Nervous System Infections

P. F.G. Wolffs^*, C. Vink, J. Keijdener, B. Habek, M. Reijans, G. Simons, C. A. Bruggeman, and A. J.C. van den Brule

Department of Medical Microbiology, Maastricht University Medical Center, 6202 AZ, Maastricht, The Netherlands; PathoFinder B.V., 6229 EV Maastricht, The Netherlands; Laboratory for Pathology and Medical Microbiology, PAMM Laboratories, 5623 EJ Eindhoven, The Netherlands

^* To whom correspondence should be addressed. Email: p.wolffs{at}mumc.nl.

A multiplex ligation-dependent probe amplification (MLPA) assay for simultaneous detection of 6 virus species was developed and tested on clinical cerebrospinal fluids. The assay, termed MeningoFinder, showed an accordance of 97%, a concordance of 96%, an inter-laboratory sensitivity of 90% and an inter-laboratory specificity of 94% when compared to PCRs.