INTRODUCTION

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Despite the introduction of reasonably effective prophylaxis, Mycobacterium avium complex (MAC) infection is still a common cause of morbidity and shortened survival among AIDS patients. Once MAC disease occurs, antimycobacterial therapy with a combination of drugs is required; however, while a limited number of drugs are assumed to be active against MAC, solid clinical correlations have been achieved for clarithromycin only (6, 9). Thus, there is a need for reliable drug susceptibility testing in order to choose the drug(s) to be used with clarithromycin for preventing drug resistance (4).

At present, mycobacteriologists agree that susceptibility tests for MAC isolates should be quantitative (i.e., MICs should be determined). It has also been established that MICs determined in broth are more accurate than those obtained by the agar dilution method (2). The radiometric (BACTEC) broth macrodilution method takes into account these findings. However, it is quite expensive and generates radioactive waste which must be disposed of.

The Mycobacteria Growth Indicator Tube (MGIT [BBL]) method is a recently introduced, nonradiometric method for the isolation of mycobacteria from clinical specimens. The MGIT system consists of an oxygen-quenched fluorescent indicator embedded in silicon at the bottom of a tube filled with Middlebrook 7H9 broth. Actively growing mycobacteria consume the oxygen dissolved in the medium, thereby releasing the indicator, whose fluorescence can be detected when the tube is viewed with a 365-nm UV light.

Recent studies have focused on the rapid detection of Mycobacterium tuberculosis resistance to first-line drugs by the MGIT system (7, 12). Reliable results, showing an excellent correlation with reference methods, have been reported at a mean time of 5 to 6 days of test processing.

The purposes of the present study were to investigate the reliability of the MGIT system for the determination of MICs of various drugs for MAC isolates and to compare the results with those achieved by the radiometric method.

	MATERIALS AND METHODS

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Test strains. Sixteen MAC strains isolated almost entirely from AIDS patients and identified to the species level by RNA-DNA hybridization (Accuprobe; Gen-Probe, San Diego, Calif.) were evaluated in this study. Smooth, transparent colonies growing on Middlebrook 7H10 agar were subcultured for comparative susceptibility testing.

Antimicrobial agents. Clarithromycin (Abbott Laboratories, North Chicago, Ill.), clofazimine (Ciba-GEIGY, Basel, Switzerland), and rifabutin (Farmitalia-Carlo Erba, Milan, Italy) were kindly provided by their manufacturers; amikacin and ethambutol were purchased from the Sigma Chemical Co. (St. Louis, Mo.).

Radiometric method. The growth of bacteria was recorded radiometrically by using the BACTEC 460-TB system (Becton Dickinson, Sparks, Md.).

MIC determination in Middlebrook 7H12 broth. For MIC determination in broth, all the drugs were added to Middlebrook 7H12 vials in a volume of 0.1 ml per vial to achieve serial doubling concentrations. A culture was grown in a Middlebrook 7H12 broth seed vial and the results were recorded daily until the culture reached the maximum GI, and then the culture was diluted 1:100; 0.1 ml of this dilution was inoculated into each of the test vials and into one of the drug-free control vials. Such an inoculum provides an initial bacterial concentration of 10⁴ to 10⁵ CFU/ml.

MIC determination in Middlebrook 7H9 MGIT broth. (i) Preparation of mycobacterial inoculum. A few colonies, scraped off a 7H10 agar slant, were inoculated into an OADC-supplemented (MGIT OADC enrichment; Becton Dickinson) MGIT tube and incubated at 35 to 37°C. The tube was visually examined for growth (for approximately 3 to 5 days), and once the tube was turbid, the growth was diluted with sterile saline with a nephelometer to match a McFarland 0.5 standard. Then the McFarland 0.5 suspension was homogenized thoroughly and diluted 1:50 by adding 0.5 to 9.5 ml of BACTEC diluting fluid (Becton Dickinson Diagnostic Instrument Systems); this dilution was the preliminary suspension.

(ii) MGIT procedure. The preliminary suspension was diluted 1:100 by adding 0.1 to 9.9 ml of diluting fluid, and 0.1 ml of this dilution (working suspension) was inoculated into the experimental tubes and into one of the drug-free control tubes (the undiluted control). Such an inoculum provides an initial bacterial concentration of 10⁴ to 10⁵ CFU/ml. The working suspension was then diluted 1:100, and 0.1 ml was used to inoculate another MGIT drug-free tube (containing a 1:100-diluted control), which contained 1% of the bacterial population. MGIT tubes were prepared by adding 0.5 ml of the MGIT OADC enrichment and 0.1 ml of the inoculum as described previously. Then 0.1 ml of antibiotic solution was added to each of the test tubes to obtain serial doubling concentrations. All tubes were incubated at 37°C and examined daily for fluorescence by placing them on a 365-nm UV transilluminator (UVP, Inc.). They were compared with a positive control tube which had been prepared by adding 5 ml of a 0.4% sodium sulfite solution to an empty MGIT tube. An uninoculated MGIT tube was used as a negative control. Fluorescent tubes, showing a bright orange color on the bottom and a reflection on the meniscus, that most closely resembled the positive control tube were considered positive. The MIC was considered interpretable when the 1:100-diluted control became positive. This requirement was usually fulfilled between days 6 and 8 of incubation. If the undiluted control became positive earlier than day 4, the tube was considered overinoculated. Similarly, if the 1:100-diluted control did not show fluorescence by day 8 of incubation, the tube was considered underinoculated. In both cases, the assays were repeated.

MIC validation. Samples were plated onto 7H10 agar to establish the CFU counts from the 1:100-diluted working suspension and, for three strains, from the test tubes at the end of the test. This was done to verify that the inoculum provided an initial bacterial concentration of 10² to 10³ CFU/ml and that the MIC was the lowest drug concentration in the presence of which the growth did not exceed 1% of the initial bacterial population.

	RESULTS

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Reading and interpretation of MGIT-determined MICs. The turnaround times for susceptibility test results ranged from 6 to 8 days (median, 7 days) for the MGIT and from 5 to 7 days (median, 6 days) for the BACTEC system. After the incubation period, the majority of strains demonstrated clear results (presence or absence of strong fluorescence at the bottoms of the tubes) which were easy to interpret. Occasionally, when weak fluorescence was observed, it was recorded as positive.

Comparison of BACTEC system-determined and MGIT-determined MICs. The agreements between the radiometric method and the MGIT system, within ±1 log₂ dilution, were 100, 100, 88, 63, and 44% for amikacin, clofazimine, rifabutin, clarithromycin, and ethambutol, respectively (Table 1). Within ±2 log₂ dilutions, agreements with BACTEC system-determined MICs increased to 100% for all the tested drugs. The MICs obtained by both methods are shown in Table 2. Excellent agreement between BACTEC system- and MGIT-determined MICs of amikacin, clofazimine, and rifabutin was demonstrated, while discrepancies of more than 1 dilution step could frequently be observed for clarithromycin and ethambutol. MGIT-determined MICs were, on average, 2 log₂ dilutions lower for clarithromycin and 2 log₂ dilutions higher for ethambutol than BACTEC system-determined MICs (Fig. 1 and 2).

View larger version (14K): [in this window] [in a new window]   FIG. 1.   MICs of amikacin, clarithromycin, and clofazimine for 16 MAC strains as determined by the BACTEC and MGIT systems.

View larger version (14K): [in this window] [in a new window]   FIG. 2.   MICs of ethambutol and rifabutin for 16 MAC strains as determined by the BACTEC and MGIT systems.

Comparison of BACTEC system-determined and MGIT-determined MICs according to the BACTEC interpretive breakpoints. The qualitative susceptibility results from both tests (i.e., resistance and susceptibility), based on the thresholds adopted for interpretation of BACTEC system-determined MICs (3), are given in Table 3. Excellent agreement was demonstrated for all MAC clinical isolates for clarithromycin and rifabutin (100%). For clofazimine and amikacin, discrepant results (88 and 50% agreement, respectively) occurred because most of the MICs determined by both systems were close to the resistance breakpoint concentrations. In this case, even a 1-dilution difference made some strains shift from the moderate-susceptibility category into the resistance category. With ethambutol, discrepant results were obtained with the majority of strains tested: unlike the strains treated with clarithromycin, a considerable number of strains categorized as susceptible to ethambutol by the BACTEC system were revealed to be resistant when tested by the MGIT system.

MGIT-determined MIC validation. We recorded an average count of 2,186 CFU/ml (range, 600 to 5,004 CFU/ml) by sampling and plating onto 7H10 agar from the 1:100-diluted working suspension, while the tubes at the end of the test yielded an average count of 2,498 CFU/ml (range, 20 to 6,301 CFU/ml). The MGIT-determined MICs correlated well with those based on CFU counts. The MIC was the lowest drug concentration in the presence of which the growth did not exceed 1% of the initial bacterial population.

	DISCUSSION

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MAC disease will remain a therapeutic challenge for AIDS-infected patients. Although a linear correlation between clinical response and MIC results has not yet been found (10), therapy still relies on in vitro drug susceptibility test results and therefore requires rapid and standardized methods (8). The MGIT system was evaluated for its ability to determine MICs of a range of antibiotics for MAC isolates, and the results were compared with those of the radiometric method. The test was technically easy to perform, and the results were easy to interpret. Inoculum preparation and susceptibility turnaround time were similar to those of the BACTEC procedure. Within ±1 log₂ dilution, MICs of amikacin, clofazimine, and rifabutin showed 100, 100, and 88% agreement, respectively. In contrast, for clarithromycin and ethambutol, MIC agreements within ±1 log₂ dilution were 63 and 44%, respectively. Within ±2 log₂ dilutions, agreement with BACTEC system-determined MICs increased to 100% for all the tested drugs. Moreover, if MGIT-determined MICs were evaluated according to the thresholds adopted for the interpretation of BACTEC system-determined ones, ethambutol was the only drug for which susceptible strains were frequently misclassified as resistant. The cause of such a discrepancy has not been determined. Recent data on M. tuberculosis complex drug susceptibility testing by the MGIT system seem to support our findings. Major discrepancies have been reported for ethambutol by different authors (1, 5, 11) who observed that about 2 to 5% of the strains classified as resistant by the MGIT were classified as susceptible by the BACTEC system. We suggest that the new detection system, the medium's richness, and ethambutol's mechanism of action (bacteriostatic instead of bactericidal) could help to explain this phenomenon. Moreover, the possibility of a quenching effect on ethambutol by one or more components of the MGIT system (perhaps the fluorescent indicator or the silicon embedded at the bottom of the tube) should also be investigated.

In addition, we believe that our technique is open to further improvement as kinetic reading of the MGIT test tubes by the newly developed BACTEC MGIT 960 system (Becton Dickinson) becomes available.

It is concluded that the MGIT system shows promise as a nonradiometric alternative to the BACTEC system for quantitative susceptibility testing of MAC isolates. However, additional studies are needed to confirm our results and to determine optimal criteria for the interpretation of ethambutol MICs.