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Journal of Clinical Microbiology, December 1999, p. 4186-4188, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Hafnia alvei with the MicroScan WalkAway System

L. A. Rodríguez,1,* J. Vivas,1 C. S. Gallardo,1 F. Acosta,2 L. Barbeyto,3 and F. Real2

Departamento de Biología Funcional y Ciencias de la Salud, Facultad de Ciencias, Universidad de Vigo, Campus de Ourense,1 and Laboratorio de Microbiología, Hospital Cristal Piñor,3 Ourense, and Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Las Palmas de Gran Canaria,2 Spain

Received 28 January 1999/Returned for modification 5 June 1999/Accepted 21 August 1999


    ABSTRACT
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Hafnia alvei is a gram-negative facultatively anaerobic bacillus that belongs to the family Enterobacteriaceae. This organism is a causative agent of intestinal disorders and is found in different environments. H. alvei has received increased clinical attention as a cause of different infections in humans. This study was performed to compare the MicroScan WalkAway automated identification system in conjunction with the new MicroScan Combo Negative type 1S panels with conventional biochemical methods for identification of 21 H. alvei strains. The MicroScan WalkAway system was found capable of correctly identifying 20 of the 21 strains tested.


    TEXT
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Hafnia alvei is a gram-negative facultatively anaerobic bacillus that belongs in the family Enterobacteriaceae. It is suspected to cause a variety of intestinal disorders, including gastroenteritis (2, 21, 24, 28, 35). H. alvei has also been isolated from various mammals (29), fish (10, 26), birds (11, 20), soil, water (4, 28, 30), and a number of foods (6, 8, 14, 23, 32). H. alvei possesses several different virulence mechanisms, which are similar or identical to those of other gram-negative enteropathogens (3). In humans, H. alvei is a recognized cause of a number of illnesses, including pneumonia (13), meningitis (17), abscesses (1), and septicemia (16).

Recently, automated systems have been developed to identify gram-negative bacteria (9, 15, 18, 19, 22, 27, 33), but the reports about the evaluation of these systems did not include a large number of H. alvei strains. The MicroScan WalkAway (Dade MicroScan, Inc., Sacramento, Calif.) is an automated, commercially available system for rapid identification and susceptibility testing of gram-negative bacilli and has received favorable reports relative to the identification of these bacteria (5, 11, 15, 22, 25). MicroScan has recently marketed MicroScan Combo Negative type 1S panels. The panels are designed to identify to the species level aerobic or anaerobic facultative gram-negative bacilli. The system uses fluorogenic substrates and a pH indicator to detect bacterial enzymatic activity. The purpose of this study was to evaluate the ability of the MicroScan WalkAway system in conjunction with the new Combo Negative type 1S panels to identify H. alvei strains.

Bacterial strains. Twenty-one Hafnia alvei strains were selected for testing (Table 1). The strains were identified with the MicroScan WalkAway system and were tested in parallel by standard reference procedures (31). The strains were routinely cultured on Trypticase soy agar (TSA; Cultimed) at 37°C for 24 h and stored on TSA slants at 4°C under mineral oil and frozen at -70°C with 15% glycerol.

                              
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  		TABLE 1.   Origin and sources of H. alvei strains used in this study

MicroScan panels. Conventional MicroScan Negative Combo type 1S panels were inoculated with the strains by the turbidity standard technique. The panels were incubated for 24 h at 35°C within the MicroScan WalkAway system. All procedures were performed according to the manufacturer's directions (7).

Comparison of biochemical tests. The following biochemical tests were performed: D-glucose, sucrose, D-sorbitol, raffinose, L-rhamnose, L-arabinose, myoinositol, D-adonitol, and melibiose; urease; hydrogen sulfide (H2S) production; indole production; decarboxylation of lysine and ornithine; arginine dihydrolase; tryptophan deaminase (TDA); esculin hydrolysis; Voges-Proskauer (VP); utilization of citrate; o-nitrophenyl-beta -D-galactopyranoside (ONPG); and OF-glucose.

Results from comparison of different assay systems in testing important biochemical characteristics for the identification of H. alvei strains are listed in Table 2.

                              
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  		TABLE 2.   Comparison between MicroScan WalkAway system and conventional laboratory tests to evaluate important biochemical characteristics for identification of H. alvei strains

The MicroScan identification patterns for the 20 strains correctly identified as H. alvei were positive for the fermentation of D-glucose and L-arabinose, decarboxylation of lysine and ornithine, and utilization of citrate and OF-glucose and were negative for fermentation of sucrose, D-sorbitol, raffinose, myoinositol, D-adonitol, and melibiose; hydrogen sulfide (H2S) production; indole production; and TDA; and were variable for fermentation of L-rhamnose, urease, arginine dihydrolase, esculin, VP, and ONPG.

The MicroScan WalkAway system was able to identify 20 of 21 of the H. alvei strains tested (95%), and only 1 strain, H. alvei 14-67, was misidentified as a rare biotype. Strain 14-67 was negative in the L-rhamnose and urease tests, the same as strain 11-69, but in the case of strain 14-67, the MicroScan system interpreted the urease test as positive, which produced a misidentification as a rare biotype. In the case of strain 11-69, the urease test was negative, and the final identification was H. alvei, with a probability of 99.9%.

Initially, the MicroScan system classified as a rare biotype strains 1967-82, F-4319, 30-65, and 7-68. The arginine test was initially interpreted as positive, but after a subsequent manual reading of the panels by specialized personnel in the center, it was recorded as negative. Therefore, the final identification was H. alvei, with a probability of 99.9%.

Although H. alvei infections are relatively rare, their clinical importance is well documented. More accurate and reliable methods with which to rapidly identify these organisms need to be developed. Some studies on the reliability of automated systems have shown good results, but insofar as the H. alvei strains are concerned, only small numbers were evaluated (34, 36, 37), with the unique exception of Kelly et al. (12), who, using 38 Hafnia alvei strains, obtained results similar (92%) to those in our study.

On the whole, the MicroScan WalkAway system in conjunction with the new Combo Negative type 1S panels proved to be very useful and reliable in identifying H. alvei strains of different origins. This system correctly identified 20 of the 21 strains tested in this study (95%). In 16 of 21 tests analyzed, the correlation between the MicroScan system and conventional tests was 100%. Occasionally, the erroneous test results were important enough to result in a misidentification. Discrepancies might be expected because conventional tests may represent standard tubed media read after overnight incubation, thus accepting some loss of precision and accuracy for the sake of convenience. Such procedures could not represent appropriate reference methods for evaluating automatic systems. The low percentages of correlation in some tests (urease, 23.8%; VP, 14.2%; citrate, 4.7%; and ONPG, 47.6%) did not seem to affect the final identification, although they should be considered if other gram-negative bacteria are tested.

The arginine dihydrolase test presented interpretation difficulties. This test caused a misidentification of one strain as a rare biotype on the MicroScan system. Therefore, it is recommended that this test be read manually in the event of a positive result in the automatic reading.

Generally, the L-rhamnose test is positive for H. alvei, although there are a few samples that are L-rhamnose negative (strains 11-69 and 14-67). When the L-rhamnose test is negative, special attention should be paid to the urease test, because a positive urease result gives a rare biotype in the MicroScan system, while a positive L-rhamnose result always gives the identification of H. alvei, independent of the urease test.

In conclusion, the results of this study confirm that the MicroScan WalkAway system, in conjunction with the new MicroScan Combo Negative type 1S panels, is reliable for identification of H. alvei strains.


    ACKNOWLEDGMENTS

We thank J. Michael Janda for critical review of the manuscript. The assistance of the staff of the Clinical Microbiology Laboratory at the Hospital Cristal Piñor Ourense is greatly appreciated. We thank Cultimed-Spain for providing culture media.

L. A. Rodríguez is the recipient of a University of Vigo grant.


    FOOTNOTES

* Corresponding author. Mailing address: Área de Microbiología, Departamento de Biología Funcional y Ciencias de la Salud, Universidad de Vigo, Campus de Ourense, Las Lagunas, 32004 Ourense, Spain. Phone: 988 387066. Fax: 988 387001. E-mail: lalopez{at}uvigo.es.


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Journal of Clinical Microbiology, December 1999, p. 4186-4188, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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