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Journal of Clinical Microbiology, January 2000, p. 40-43, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Convenient Test for Screening Metallo-beta -Lactamase-Producing Gram-Negative Bacteria by Using Thiol Compounds

Yoshichika Arakawa,1,* Naohiro Shibata,1 Keigo Shibayama,1 Hiroshi Kurokawa,1 Tetsuya Yagi,1 Hiroshi Fujiwara,1 and Masafumi Goto2

Department of Bacterial and Blood Products, National Institute of Infectious Diseases, Tokyo,1 and Faculty of Pharmaceutical Sciences, Kumamoto University, Kumamoto,2 Japan

Received 28 June 1999/Returned for modification 21 August 1999/Accepted 22 September 1999


    ABSTRACT
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Abstract
Introduction
Materials and Methods
Results and Discussion
References

A simple disk diffusion test was constructed for detection of IMP-1-type metallo-beta -lactamase-producing gram-negative bacteria. Two Kirby-Bauer disks containing ceftazidime (CAZ) and a filter disk containing a metallo-beta -lactamase inhibitor were used in this test. Several IMP-1 inhibitors such as thiol compounds including 2-mercaptopropionic acid, heavy metal salts, and EDTA were evaluated for this test. Two CAZ disks were placed on a Mueller-Hinton agar plate on which a bacterial suspension was spread according to the method recommended by the National Committee for Clinical Laboratory Standards. The distance between the disks was kept to about 4 to 5 cm, and a filter disk containing a metallo-beta -lactamase inhibitor was placed near one of the CAZ disks within a center-to-center distance of 1.0 to 2.5 cm. For IMP-1-producing strains, the growth-inhibitory zone between the two disks expanded, while no evident change in the shape of the growth-inhibitory zone was observed for CAZ-resistant strains producing serine beta -lactamases such as AmpC or SHV-12. As a result, 2 to 3 µl of undiluted 2-mercaptopropionic acid or mercaptoacetic acid able to block IMP-1 activity gave the most reproducible and clearest results, and CAZ-resistant strains producing AmpC or extended-spectrum beta -lactamases were distinguishable from IMP-1 producers by this test. A similar observation was made with IMP-1-producing clinical isolates such as Serratia marcescens, Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Enterobacter aerogenes, Citrobacter freundii, Proteus vulgaris, Pseudomonas aeruginosa, Pseudomonas putida, Acinetobacter spp., and Alcaligenes xylosoxidans. The specificity and sensitivity of this test were comparable to those of PCR analysis using blaIMP-specific primers. Therefore, this convenient test would be valuable for daily use in clinical laboratories.


    INTRODUCTION
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Abstract
Introduction
Materials and Methods
Results and Discussion
References

Carbapenem-resistant gram-negative bacterial species such as Serratia marcescens and Pseudomonas aeruginosa have emerged in Japan, and these isolates usually produce IMP-1 metallo-beta -lactamase (7, 9, 13, 15, 17). The blaIMP genes responsible for the IMP-1 production are usually mediated by integrons carried by transferable large plasmids (1). About 4.4% of S. marcescens strains and 1.3% of P. aeruginosa strains have already acquired IMP-1 productivity in Japan (manuscript in preparation), and transmissions of the blaIMP gene cassette have been observed among various gram-negative rods (18). Since IMP-1 producers tend to demonstrate a wide range of resistance to various broad-spectrum beta -lactams including the oxyimino cephalosporins, cephamycins, and carbapenems, early recognition of IMP-1 producers is very important for rigorous infection control (3). The worldwide spread of this kind of organism is becoming a general concern, since several metallo-beta -lactamase-producing gram-negative bacteria have recently been reported outside Japan (5, 11, 19). Indeed, PCR analyses usually give reliable and satisfactory results (18), but this method is of limited practical use for daily application in clinical laboratories because of the cost. Thus, the development of a simple and inexpensive testing method for screening of IMP-1 producers has become necessary.


    MATERIALS AND METHODS
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Abstract
Introduction
Materials and Methods
Results and Discussion
References

Bacterial strains. Clinically isolated ceftazidime (CAZ)-resistant (MIC, >64 µg/ml) gram-negative bacterial strains were used in the test. Several of these isolates were later found to carry the blaIMP gene by PCR. A well-characterized extended-spectrum beta -lactamase (ESBL) (SHV-12) producer and AmpC hyperproducers were also used as the control strains. A list of the bacterial strains tested in this study is shown in Table 1.

                              
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  		TABLE 1.   Strains used in this study

Evaluation of metallo-beta -lactamase inhibitors. CuCl2, FeCl2, EDTA, and thiol compounds including mercaptoacetic acid, 2-mercaptopropionic acid, and mercaptoethanol were used and evaluated for IMP-1 inhibition, because these agents have been reported to block metallo-beta -lactamase (2, 6, 12, 16). A colony of each bacterial strain was suspended and diluted with Mueller-Hinton (MH) broth to 106 CFU/ml and spread on an MH agar plate with a cotton swab according to the protocol recommended by the National Committee for Clinical Laboratory Standards (14). Two commercially supplied Kirby-Bauer (KB) disks, each containing 30 µg of CAZ (Eiken Co. Ltd., Tokyo, Japan), were then placed on the plates. The distance between the two CAZ disks was kept at about 4 to 5 cm, and a filter disk was placed near one of the CAZ disks within a center-to-center distance of 1.0 to 2.5 cm. Two to five microliters of each inhibitor solution was added to the filter disk on the agar, and each agar plate was incubated at 37°C overnight. The concentration and amount of each inhibitor solution added to the filter disk were as follows: for CuCl2, 100 mM (5 µl); for FeCl2, 100 mM (5 µl); for EDTA, 100 mM (5 µl); and for thiol compounds, an undiluted solution (2 to 3 µl).

PCR analysis. CAZ-resistant strains used in this study were tested by PCR analysis to confirm the presence of the blaIMP gene according to the method of Senda et al. (18) by using a new set of PCR primers (5'-ACCGCAGCAGAGTCTTTGCC-3' and 5'-ACAACCAGTTTTGCCTTACC-3').


    RESULTS AND DISCUSSION
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Abstract
Introduction
Materials and Methods
Results and Discussion
References

Among the metallo-beta -lactamase inhibitors used in this study, 2-mercaptopropionic acid gave the clearest results, because this chemical agent blocked IMP-1 activity very strongly even at a low concentration (6). Mercaptoacetic acid also gave a clear result, but its inhibitory effect was slightly weaker than that of 2-mercaptopropionic acid. By using 2-mercaptopropionic acid, apparent growth-inhibitory zones were observed with all IMP-1-producing strains tested, including S. marcescens MKDM17, Klebsiella pneumoniae MKD115, and P. aeruginosa MKAM12, while no distinct change in the appearance of the growth-inhibitory zone was observed for CAZ-resistant strains producing AmpC or SHV-12 (Fig. 1). Of 3,222 S. marcescens isolates and 2,533 P. aeruginosa isolates, 141 and 88 isolates carrying blaIMP, respectively, demonstrated an expansion of the growth-inhibitory zone by 2-mercaptoacetic acid, and Escherichia coli strains producing Toho-1 or MEN-1 that show resistance to cefotaxime were distinguishable from IMP-1 producers by this method (data not shown). The other IMP-1 producers belonging to the gram-negative bacterial species also showed results similar to those observed with IMP-1-producing S. marcescens and P. aeruginosa strains, as shown in Fig. 2. However, relatively weak and ambiguous growth-inhibitory zones appeared for IMP-1-producing Citrobacter freundii and Enterobacter cloacae, even when two disks containing CAZ and 2-mercaptoacetic acid, respectively, were placed as close together as 1 cm (from center to center) (Fig. 2). This may be due to the hyperproduction of AmpC and/or to a change in membrane permeability in these bacteria. Further study is needed to improve the method for these strains, though IMP-1-producing strains of C. freundii and E. cloacae are still very rare.


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  		FIG. 1.   Inhibitory effects of 2-mercaptopropionic acid (2-MPA) on IMP-1 producers and non-IMP-1 producers. Three CAZ-resistant strains belonging to the gram-negative bacterial species P. aeruginosa, S. marcescens, and K. pneumoniae and producing IMP-1 metallo-beta -lactamase or serine-beta -lactamases (SHV-12 or AmpC) were tested. For each IMP-1 producer, a distinct growth-inhibitory zone appeared between the KB disk containing CAZ and the filter disk containing 2-MPA (left column). No change is evident around the two KB disks containing CAZ with or without 2-MPA for each serine beta -lactamase producer (right column).


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  		FIG. 2.   Appearance of growth-inhibitory zone in IMP-1-producing strains by use of CAZ and 2-mercaptopropionic acid (2-MPA). Various levels of growth inhibition were observed in the IMP-1-producing gram-negative bacterial species tested. Marked growth inhibitions were observed in Acinetobacter sp., Alcaligenes xylosoxidans, Enterobacter aerogenes, E. coli, Proteus vulgaris, and Pseudomonas putida, whereas weak and ambiguous growth inhibitions were observed in C. freundii and E. cloacae.

Heavy metal salts such as CuCl2 and FeCl2 usually formed ring-shaped areas of precipitation around the filter disk and demonstrated their own bactericidal activity, while the growth-inhibitory zone expanded to the disk containing CAZ, as shown in Fig. 3A. The inhibitory effects of both heavy metal salts were similar, but the results were ambiguous in several strains. HgCl2 itself has rather strong bactericidal activity and yielded better results than CuCl2 and FeCl2 in the preliminary tests. However, the use of Hg2+ salt is not recommended from the viewpoint of human health and environmental conservation.



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  		FIG. 3.   (A) Inhibitory effects of FeCl2 on IMP-1 producers and non-IMP-1 producers. A slight expansion of growth-inhibitory zones between two disks was observed for all three IMP-1 producers (arrowheads). No change in the shape of the growth-inhibitory zone was evident for any serine-beta -lactamase producer. (B) Inhibitory effects of EDTA on IMP-1 producers. Growth-inhibitory zones between two disks appeared for all three IMP-1 producers (arrowheads) when 5 µl of 500 mM EDTA solution was added to the filter.

EDTA also created a growth-inhibitory zone between the two disks, but its appearance and reproducibility were relatively poor in several strains, even when a thick EDTA solution (500 mM) was added to the filter disk (Fig. 3B).

CAZ seemed to be the most suitable substrate for this test, because IMP-1 producers usually demonstrated high-level resistance to CAZ (MIC, >64 µg/ml) in our previous study (17, 18), and a marked inhibitory effect of thiol compounds was usually observed, as shown in Fig. 1 and 2. Indeed, any kind of broad-spectrum beta -lactam disk can be used in this test, but IMP-1 producers usually demonstrate various levels of resistance to imipenem (IPM) (MIC, 4 to >128 µg/ml). However, the inhibitory effect of thiol compounds tends to be ambiguous, especially in strains that demonstrate reduced susceptibility to IPM (MIC, 4 to 8 µg/ml) when the KB disk (IPM) is used (data not shown).

The emergence of gram-negative bacterial species with acquired resistance to various broad-spectrum beta -lactams is becoming a worldwide clinical problem. Strains producing TEM- or SHV-derived ESBLs (4, 10) usually demonstrate high-level resistance to broad-spectrum oxyimino beta -lactams such as CAZ and cefotaxime. Moreover, several K. pneumoniae strains that showed resistance to cephamycins as well as oxyimino cephalosporins were also found to produce AmpC-type beta -lactamases such as MOX-1 (8). In Japan, furthermore, the emergence of carbapenem-resistant gram-negative bacterial strains in species such as S. marcescens or P. aeruginosa is becoming a clinical threat. Some of these isolates produce IMP-1 metallo-beta -lactamase, and these strains tend to demonstrate a wide range of resistance to various broad-spectrum cephalosporins, cephamycins, and carbapenems (7, 9, 15, 17). Recently, gram-negative bacterial strains that were speculated to produce metallo-beta -lactamases very similar to IMP-1 were also isolated in the United Kingdom, Italy, and Singapore (5, 11, 19). Thus, there is a need to develop a simple and specific method to distinguish IMP-1 producers from other bacteria showing a similar antibiotic resistance profile through the production of AmpC, ESBLs, or Toho-1-type beta -lactamases. Indeed, PCR analysis usually gives satisfactory results in the detection of IMP-1 producers (7, 17), but it is not suitable for daily testing in clinical laboratories due to the cost. Therefore, the method described in this study is very helpful for screening IMP-1-producing strains in daily clinical laboratory testing.


    ACKNOWLEDGMENT

This work was supported by a grant (The Research Project for Emerging and Re-Emerging Infectious Diseases: Molecular Analyses of Drug-Resistant Bacteria and Establishment of Rapid Identification Methods, 1997-1999) from the Ministry of Health and Welfare of Japan.


    FOOTNOTES

* Corresponding author. Mailing address: Department of Bacterial and Blood Products, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan. Phone: 81-42-561-0771, ext. 500. Fax: 81-42-561-7173. E-mail: yarakawa{at}nih.go.jp.


    REFERENCES
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Abstract
Introduction
Materials and Methods
Results and Discussion
References

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Journal of Clinical Microbiology, January 2000, p. 40-43, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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