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Journal of Clinical Microbiology, January 2000, p. 467-467, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
LETTERS TO THE EDITOR
Detection of Bordetella holmesii Using
Bordetella pertussis IS481 PCR Assay
 |
LETTER |
After our manuscript comparing the performance of PCR, culture,
and direct fluorescent-antibody testing for Bordetella
pertussis (3) went to press, we obtained six isolates
of Bordetella holmesii for the purpose of testing with our
B. pertussis IS481 PCR assay. This request for
isolates was prompted by the recent report by Yih et al. (6)
on the isolation of B. holmesii from nasopharyngeal (NP)
specimens from patients with pertussis-like cough. Previously, B. holmesii has been associated with septicemia in immunocompromised patients (5) and was isolated from the sputum of patients
with respiratory failure (4). Yih et al. isolated B. holmesii infrequently from NP specimens during their evaluation
(0.26% positivity rate, compared to 6.6% positivity rate for B. pertussis) (6).
B. holmesii isolates (kindly provided by H. George,
Massachusetts Department of Public Health) were grown on Trypticase soy agar plates containing 5% sheep blood for 48 h at 35°C. Colony picks were suspended in sterile water and were tested with the B. pertussis PCR assay as previously described (3).
Initially, the quantity of organisms added to PCRs was not determined,
but it was estimated to be at least 100 to 1,000 CFU per reaction. All
six B. holmesii isolates generated strong positive results with the B. pertussis IS481 PCR assay. To
evaluate the limit of detection, suspensions of two B. holmesii isolates were prepared, and cell concentrations were
estimated based on McFarland turbidimetric standards. Aliquots of
serial dilutions were tested with the B. pertussis PCR
assay. The analytical sensitivity ranged from 0.06 to 0.3 CFU per PCR,
which is similar to the sensitivity we previously reported for B. pertussis (3).
B. pertussis PCR assays that target the IS481
repetitive element may cross-react with B. holmesii. Our PCR
assay utilizes previously described primers and probe. The sequences of
the upstream and downstream primers were previously described by Glare
et al. (1) and He et al. (2), respectively. The
sequence of our capture probe is identical to that of the downstream
primer used by Glare et al. (1). We have not determined
whether actual NP specimens from patients infected or colonized with
B. holmesii would generate a positive result with our
B. pertussis PCR test. We have not isolated B. holmesii from NP swab specimens in our laboratory. However, our
Regan-Lowe transport medium contains 40 µg of cephalexin per ml, to
which B. holmesii is reportedly susceptible (E. Mazengia, H. George, E. Silva, and J. Peppe, Abstr. 99th Gen. Meet. Am. Soc.
Microbiol. 1999, abstr. A-113, p. 24, 1999). It is possible that some
of the patients in our earlier study (3) who were positive
by PCR alone were infected or colonized with B. holmesii and
not with B. pertussis. However, if the B. holmesii prevalence in our study were similar to that reported in
Massachusetts, the number of positive specimens would be very low.
The clinical and public health significance of B. holmesii
as a respiratory pathogen in otherwise healthy individuals remains to
be determined. In fact, it has yet to be proven conclusively that
B. holmesii causes respiratory disease. Nonetheless, these data suggest that B. holmesii may confound B. pertussis PCR assays that target IS481, requiring
redefinition of performance characteristics or modification of assay methods.
| |
FOOTNOTES |
*
Phone: (319) 335-4500
Fax: (319) 335-4555
E-mail: michael-loeffelholz{at}uiowa.edu
| |
REFERENCES |
| 1.
|
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Loeffelholz, M. J.,
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Weyant, R. S.,
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|
| | | | |
Mike J. Loeffelholz*
Curt J. Thompson
Karla S. Long
Mary J. R. Gilchrist
State Hygienic Laboratory
University of Iowa
Iowa City, Iowa 52242
|
Journal of Clinical Microbiology, January 2000, p. 467-467, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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