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Gram-positive anaerobic cocci (GPAC) are commonly present in clinical specimens and are often mixed with other anaerobic or aerobic bacteria. GPAC can be involved in chronic infections of the upper respiratory tract, ears, mastoid, sinuses and teeth, intra-abdominal and female pelvic regions, subcutaneous and soft tissue abscesses, and diabetic foot ulcers, as well as in osteomyelitis and arthritis (2, 3, 7). Peptostreptococcus magnus, one of the GPAC species, is part of the normal flora of the human mucocutaneous surfaces. Although P. magnus is the most frequently isolated GPAC from clinical specimens, as well as in pure culture and from normally sterile sites, it has been scarcely reported as a cause of septicemia or native- or prosthetic-valve endocarditis (7, 8). Automated blood culture systems are developed in order to culture a broad variety of microorganisms. The anticipated pitfall of these systems is the inability to recover fastidious microorganisms with low CO₂ production, such as Brucella species (12). Recently, however, false-negative cultures in an automated blood system were also reported for Pseudomonas aeruginosa as well (5). We report here two cases of prosthetic-valve endocarditis due to P. magnus which was recovered after culturing the surgically removed infected valve, whereas BacT/Alert blood cultures, incubated for 14 days, remained negative. Therefore, we assessed the capability of the BacT/Alert system to detect GPAC bacteremia.

Case 1. The first case was a 65-year-old man who suffered from a mitral valve incompetence due to degenerative changes. The patient was scheduled for mitral valvuloplasty and a venous single graft coronary bypass on the posterior descending branch. Routine preoperative screening for infectious dental foci was negative. During the operation, valvuloplasty appeared to give insufficient results, so the mitral valve was excised and replaced with a prosthetic heart valve (Medtronic Hall number 29). The patient received cefamandole as routine surgical prophylaxis for 2 days. The postoperative course was unremarkable, and the patient was discharged 19 days after surgery. On day 23 the patient was admitted to local hospital A because of a high-grade fever (39.2°C) with cold chills. On physical examination he was hemodynamically stable and clear clicks of the prosthetic valve were present without any murmurs. The wound of the venectomy on the left leg was slightly inflamed. C-reactive-protein (CRP) levels were increased up to 157 U/liter, there was a mild normocytic anemia of 5.6 mmol/liter, and there was an increased leukocyte count of 34.2 × 10⁹/liter, with band forms. In total, 14 blood culture sets were taken and incubated in the BACTEC 9240 system. All cultures remained negative after incubation for 2 weeks. A culture taken from the venectomy wound revealed Staphylococcus aureus, and flucloxacillin was started (6,000 mg/24 h). Despite treatment, the patient remained febrile. On day 33 after surgery, the patient developed a sudden, rapidly progressive pulmonary congestion and was referred to our hospital. On transesophageal echocardiography (TEE), a severe paravalvular leakage of the prosthetic mitral valve was demonstrated due to an almost complete dehiscence. On this valve, vegetations were observed. Two blood culture sets were taken and incubated in the BacT/Alert system, used in our laboratory. At reoperation the infected prosthetic valve was removed, the infected area was surgically cleaned, and a new Medtronic Hall prosthetic mitral valve was implanted. The removed valve was immediately transported to our laboratory in a sterile container. Several impression smears were made. Gram staining showed some destructed cells and no bacteria. Histopathologic preparations showed only degenerative changes and no bacteria. Valve parts were ground and cultured on Columbia blood agar (Oxoid, Basingstoke, England), on chocolate agar (GC Agar Base; Oxoid), and in BBL Thioglycollate Medium (Becton Dickinson, Cockeysville, Md.). Incubation was done at 5% CO₂ and anaerobically at 37°C for up to 7 days. The patient was treated with vancomycin (2,000 mg/24 h), gentamicin (240 mg/24 h), and rifampin (1,200 mg/24 h). Despite maximal support, the patient died 2 days later because of untreatable cardiogenic shock. On autopsy, a dilated hypertrophic left and right ventricle was found with signs of an old infarction in the posterior wall with recent extension to the lateral side of the septum. The grafts on the posterior descending branch and the descending anterior branch were patent. There was no dehiscence of the reimplanted prosthetic heart valve. In the BacT/Alert system no growth was detected after incubation for 14 days. Subcultures of the four BacT/Alert bottles on Columbia blood agar and chocolate agar were also negative. Anaerobic culture of the removed infected heart valve yielded a few colonies of large gram-positive cocci after 48 h of incubation on blood agar. Also, the Thioglycollate Medium showed growth after 48 h. The cocci were biochemically inert, sensitive for kanamycin, and resistant for sodium polyanethol sulfonate (SPS) and were identified as P. magnus. The identification was confirmed by 16S rRNA sequencing (National Institute of Public Health, Bilthoven, The Netherlands).

Case 2. The second case was a 39-year-old male who underwent an aortic valve replacement because of severe aortic regurgitation. Routine preoperative screening for infectious dental foci was negative. Two months after the operation dehiscence of the prosthetic valve was seen on TEE with paravalvular leakage. A few months later there were progression of the dehiscence and valvular vegetations, and aortic root abscesses could be detected by TEE. Blood cultures collected at local hospital B and incubated in the BACTEC 9240 remained negative, and no antibiotic therapy was started. The patient was referred to our hospital for a second aortic valve (Sorin 29) replacement. On admission, the CRP level was elevated (104 U/liter), and blood cultures were obtained. The removed valve was handled according to standard operating procedures as described above (case 1). The Gram staining of impression smears showed some leukocytes and sporadic gram-positive cocci. Empirical treatment was started with vancomycin (2,000 mg/24 h) and gentamicin (240 mg/24 h). Seven blood culture sets incubated for 14 days in the BacT/Alert system remained negative. P. magnus grew after 96 h of culture of the valve vegetations on blood agar and in Thioglycollate Medium. The patient was started on penicillin G (12,000,000 U/24 h) and metronidazole (1,500 mg/24 h). A few days later a third operation was necessary because of the presence of abscesses detected by TEE. A bioprosthesis (Freestyle) was inserted. The postoperative period was without complications, and the patient recovered completely. After 6 weeks of treatment, the CRP level was normal (<3 U/liter), and the antibiotics were discontinued.