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Journal of Clinical Microbiology, February 2000, p. 910-913, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Diagnostically and Experimentally Useful Panel of Strains from the Burkholderia cepacia Complex

Eshwar Mahenthiralingam,1,* Tom Coenye,2 Jacqueline W. Chung,1 David P. Speert,1 John R. W. Govan,3 Peter Taylor,4 and Peter Vandamme2

Departments of Paediatrics and Pathology, University of British Columbia, Vancouver, British Columbia, Canada1; Laboratory of Microbiology, University of Ghent, Ghent, Belgium2; Department of Medical Microbiology, University of Edinburgh Medical School, Edinburgh, United Kingdom3; and St. George Hospital and University of New South Wales, Kogorah, New South Wales, Australia4

Received 7 May 1999/Returned for modification 26 August 1999/Accepted 1 November 1999


    ABSTRACT
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Two new species, Burkholderia multivorans and Burkholderia vietnamiensis, and three genomovars (genomovars I, III, and IV) currently constitute the Burkholderia cepacia complex. A panel of 30 well-characterized strains representative of each genomovar and new species was assembled to assist with identification, epidemiological analysis, and virulence studies on this important group of opportunistic pathogens.


    TEXT
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The gram-negative bacterium Burkholderia cepacia is a problematic pathogen in patients with cystic fibrosis (CF) (18) or chronic granulomatous disease (CGD) (28) and in other vulnerable individuals (31). At least five genomovars constitute isolates which were previously classified as B. cepacia, and these strains have been collectively designated the B. cepacia complex (30). Bacteriological identification, epidemiological tracking, and virulence studies will all benefit from the use of a defined set of strains representative of each genomovar.

Assembly of a strain panel. A B. cepacia complex strain panel consisting of 30 strains representative of all five currently defined genomovars was assembled (Table 1). Strains were cultured as described previously (4, 11, 30) and deposited in the Belgium Coordinated Collections of Microorganisms/Laboratorium Microbiologie Ghent (BCCM/LMG) (http://www.belspo.be/bccm/) bacterial collection at the University of Ghent, Ghent, Belgium.

                              
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  		TABLE 1.   The B. cepacia complex strain panel

Genomovar analysis and strain typing. Genomovar testing was performed by whole-cell protein profile analysis as described previously (30). In addition, amplified fragment length polymorphism analysis (4) and sequence analysis of the recA gene (21) were used to confirm the classifications obtained by conventional analysis (30). Genetic typing of each strain was performed by random amplified polymorphic DNA (RAPD) analysis (19) and by pulsed-field gel electrophoresis (PFGE) (25) as described previously. The presence of the cable pilus subunit gene (cblA) (26) and B. cepacia epidemic strain marker (BCESM) were determined as described previously (20).

Genetic manipulation. Susceptibility to trimethoprim and transformation by electroporation with the broad-host-range vector pUC29T (32) were carried out as described elsewhere (1).

B. cepacia genomovar I. Four strains representative of this genomovar were included in the panel (Table 1). Strain ATCC 25416T, isolated from onions, has been genetically mapped (25) and well characterized phytopathologically (9). Strain ATCC 17759, also an environmental isolate, has been studied for its autoinducer production and potential for interspecies signalling (22). Strain CEP509 was recovered from a patient with CF in Sydney, New South Wales, Australia (Table 1); isolates with RAPD fingerprints identical to those of strain CEP509 were recovered from three other CF patients attending this treatment center. Strain LMG 17997 was isolated in 1976 from human urine and persisted in the urinary tract of this patient for 10 years with no clinical symptoms of infection (Table 1).

B. multivorans (formerly B. cepacia genomovar II). Eight Burkholderia multivorans strains were included in the panel (Table 1). Strain C5393 was recovered from a CF patient in Vancouver, Canada, and was not associated with patient-to-patient spread (19). Strain LMG 13010, the type strain of B. multivorans, was recovered from a Belgian CF patient and was also not associated with epidemic spread (24). Strain C1576 was recovered from a CF patient in Glasgow, Scotland, and was the index strain in an outbreak among 17 pediatric CF patients attending a treatment center in which five children died after colonization (33). Strain CF-A1-1 is a representative of an outbreak among four adult CF patients in Cardiff, Wales (23). Strain JTC was recovered from a patient with CGD and has been demonstrated to be resistant to nonoxidative killing by human neutrophils (28). Strain C1962 caused multiple brain abscesses in an immunocompetent individual (12). Strain ATCC 17616 is a soil isolate from the United States (29) and has been well characterized with regard to its metabolism, genetics, and genome structure (3, 6, 29). B. multivorans 249-2 was derived in the laboratory from ATCC 17616; it has suffered a genomic deletion resulting in a number of phenotypic alterations, including susceptibility to gentamicin (6), which is not a characteristic trait for strains of the B. cepacia complex (11).

B. cepacia genomovar III. Ten genomovar III strains were included in the panel (Table 1). Four strains from the major transmissible lineage known as ET12 (14), the cblA+ strain (26), or RAPD type 2 (19, 20) were included. Strain J2315 was the index strain from which patient-to-patient spread of this lineage was first reported in Edinburgh, Scotland (10). This strain also produces a hemolysin capable of inducing apoptosis and degranulation in human neutrophils (13). Strain BC7 was recovered from a CF patient in Toronto, Ontario, Canada, and has been studied extensively with regard to binding to mucins or respiratory epithelial cells and cable pilus virulence factor (26). Strain K56-2 was also recovered from a CF patient in Toronto and has proven to be highly amenable to genetic manipulation, enabling characterization of siderophore production (5) and genes involved in quorum sensing (16). Strain C5424 was recovered from a CF patient in Vancouver (19) and was the isolate from which the BCESM DNA was cloned and characterized (20). Strain C6433 is a representative of B. cepacia RAPD type 4 strains which have spread among CF patients in Vancouver (19). Strain C1394 was responsible for an outbreak among CF patients attending a treatment center in Manchester, England (19, 27). Strain PC184 was recovered from a pediatric CF patient attending a treatment center in Cleveland, Ohio, and was examined in one of the earliest reports of transmission of B. cepacia among patients with CF (17). Genomovar III strain CEP511 was recovered from a CF patient in Sydney, New South Wales, Australia, and is also representative of an epidemic strain which had spread among several patients (19). Strain J415 was not associated with patient-to-patient spread (8) and does not contain either the BCESM or cblA gene (Table 1). This strain was the first reported case of B. cepacia syndrome in a CF patient in the United Kingdom; however, it did not transfer to the potentially susceptible CF sibling of the child involved (8). Strain ATCC 17765 was isolated in 1964 from a urinary tract infection of a child in Bristol, England (29).

B. cepacia genomovar IV. Four genomovar IV isolates were included in the panel (Table 1). Strain LMG 14294 was isolated from sputum of a Belgian CF patient (24). A second patient from the same center carried an indistinguishable isolate; the clinical condition of both patients was stable (24). Genomovar IV strain C7322 was recovered from an adult CF patient attending a clinic in Vancouver; no other patients at this center were colonized with the same strain type (19). Strain LMG 14086 was isolated from a respirator in a hospital in the United Kingdom (4). Strain LMG 18888 is a non-CF isolate involved in an outbreak in a cardiology ward in Belgium (31).

B. vietnamiensis (formerly B. cepacia genomovar V). Four Burkholderia vietnamiensis strains, three of which were recovered from patients with CF, were included within the panel (Table 1). B. vietnamiensis PC259 was recovered from a CF patient attending a treatment center in Seattle, Washington (15), and subcultures from the same patient have been shown to invade respiratory epithelial cells in culture (2). Strain LMG 16232 was recovered from a CF patient in Sweden. Strain FC441 was recovered from a 9-year-old boy with X-linked recessive CGD who was treated in Vancouver and survived septicemia with multiple-organ involvement (Table 1). Finally, B. vietnamiensis LMG 10929 is the type strain for this species (7) and was recovered from rice rhizosphere in Vietnam.

Genetic heterogeneity. Analysis by RAPD and PFGE fingerprinting demonstrated that the strains selected for the panel were, for the most part, genetically heterogenous, representing 24 different B. cepacia complex strain types (Table 1). Strain types detected by PFGE (Fig. 1) and RAPD (Fig. 2) correlated exactly, and each method was able to type strains from all five genomovars. Three groups of strains were clonal (Table 1): B. multivorans strain ATCC 17616 and its laboratory derivative 249-2, the four ET12 strains (J2315, BC7, K56-2, and C5424), and three genomovar IV strains (LMG 14294, C7322, and LMG 14086). The remaining 21 strains within the panel each possessed a unique genetic fingerprint, and each was designated with an individual strain type (Table 1 and Fig. 1 and 2).


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  		FIG. 1.   SpeI-generated macrorestriction fragments of the B. cepacia complex strain panel separated by PFGE. Digestion and separation of macrorestricted DNA were performed as described in the text, and restriction fragments were visualized after staining with ethidium bromide. Molecular size standards were run in the lanes labelled M, and the sizes of relevant marker bands (in kilobases) are shown on the left. Strains analyzed (see Table 1) in each lane are as follows: 1, ATCC 25416T; 2, ATCC 17759; 3, CEP509; 4, LMG 17997; 5, C5393; 6, LMG 13010T; 7, C1576; 8, CP-A1-1; 9, JTC; 10, C1962; 11, ATCC 17616; 12, 249-2; 13, J2315; 14, BC7; 15, K56-2; 16, C5424; 17, C6433; 18, C1394; 19, PC184; 20, CEP511; 21, J415; 22, ATCC 17765; 23, LMG 14294; 24, C7322; 25, LMG 14086; 26, LMG 18888; 27, PC259; 28, LMG 16232; 29, FC441; 30, LMG 10929T.


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  		FIG. 2.   RAPD fingerprints generated by PCR primer 270 from DNA extracted from strains of the B. cepacia complex panel. RAPD analysis of each B. cepacia strain from the panel was performed exactly as described previously (19). Molecular size standards were run in the lanes labelled M, and the sizes of relevant marker bands (in kilobases) are indicated to the left. Strains analyzed (Table 1) in each lane are as follows: 1, ATCC 25416T; 2, ATCC 17759; 3, CEP509; 4, LMG 17997; 5, C5393; 6, LMG 13010T; 7, C1576; 8, CP-A1-1; 9, JTC; 10, C1962; 11, ATCC 17616; 12, 249-2; 13, J2315; 14, BC7; 15, K56-2; 16, C5424; 17, C6433; 18, C1394; 19, PC184; 20, CEP511; 21, J415; 22, ATCC 17765; 23, LMG 14294; 24, C7322; 25, LMG 18888; 26, LMG 14086; 27, PC259; 28, LMG 16232; 29, FC441; 30, LMG 10929T.

Strains suitable for genetic manipulation. Each genomovar possessed a strain which was readily transformable with plasmid DNA encoding a trimethoprim resistance marker, indicating that they may be useful as genetic tools (Table 1). Strain K56-2 (genomovar III) appears to be a particularly useful strain for genetic analysis. It is representative of the major epidemic CF clone (10, 14, 19) and has already proven highly amenable to molecular characterization by a number of different strategies, including transposon mutagenesis, site-directed mutagenesis by allelic exchange, and genetic complementation (16).

In terms of diversity, the panel is representative of the large variety of clinical infections, environments, and geographic locations from which B. cepacia complex strains may be recovered; however, the prevalence of each genomovar in both clinical and natural settings remains to be determined by systematic study.


    ACKNOWLEDGMENTS

This work was funded by grants from the Canadian Cystic Fibrosis Foundation (E.M. and D.P.S.) and UK Cystic Fibrosis Trust (P.V. and J.R.W.G., grant RS15; E.M. grant PJ 472). P.V. is indebted to the Fund for Scientific Research---Flanders (Belgium) for a postdoctoral research fellowship. T.C. acknowledges the bursary for advanced study from the Vlaams Instituut voor Bevordering van Wetenschappelijk-technologisch onderzoek in de Industrie (Belgium).

We are grateful to Jocelyn Bischof, Deborah Henry, and Gary Probe for excellent technical assistance and to the International B. cepacia Working Group (IBCWG) for suggestions on the choice of strains. We are indebted to the following investigators for contributing strains for inclusion within the strain panel: Jane Burns, Enevold Falsen, Tom Lessie, John LiPuma, Henry Ryley, Uma Sajjan, and Pam Sokol.


    FOOTNOTES

* Corresponding author. Present address: Cardiff School of Biosciences, Main Building, Cardiff University, P.O. Box 915, Cardiff CF1 3TL, United Kingdom. Phone: 44 01222 874190. Fax: 44 01222 874305. E-mail: MahenthiralingamE{at}cardiff.ac.uk.


    REFERENCES
Top
Abstract
Text
References

1. Burns, J. L., and L. A. Hedin. 1991. Genetic transformation of Pseudomonas cepacia using electroporation. J. Microbiol. Methods 13:215-221[CrossRef].
2. Burns, J. L., M. Jonas, E. Y. Chi, D. K. Clark, A. Berger, and A. Griffith. 1996. Invasion of respiratory epithelial cells by Burkholderia (Pseudomonas) cepacia. Infect. Immun. 64:4054-4059[Abstract].
3. Cheng, H.-P., and T. G. Lessie. 1994. Multiple replicons constituting the genome of Pseudomonas cepacia 17616. J. Bacteriol. 176:4034-4042[Abstract/Free Full Text].
4. Coenye, T., L. M. Schouls, J. R. W. Govan, K. Kersters, and P. Vandamme. 1999. Identification of Burkholderia species and genomovars from cystic fibrosis patients by AFLP fingerprinting. Int. J. Syst. Bacteriol. 49:1657-1666[Abstract/Free Full Text].
5. Darling, P., M. Chan, A. D. Cox, and P. A. Sokol. 1998. Siderophore production by cystic fibrosis isolates of Burkholderia cepacia. Infect. Immun. 66:874-877[Abstract/Free Full Text].
6. Gaffney, T. D., and T. G. Lessey. 1987. Insertion-sequence-dependent rearrangements of Pseudomonas cepacia plasmid pTGL1. J. Bacteriol. 169:224-230[Abstract/Free Full Text].
7. Gillis, M., T. V. Van, R. Bardin, M. Goor, P. Hebbar, A. Willems, P. Segers, K. Kersters, T. Heulin, and M. P. Fernandez. 1995. Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. Int. J. Syst. Bacteriol. 45:274-289[Abstract/Free Full Text].
8. Glass, S., and J. R. W. Govan. 1986. Pseudomonas cepacia---fatal pulmonary infection in a patient with cystic fibrosis. J. Infect. 13:157-158[CrossRef][Medline].
9. Gonzalez, C. F., E. A. Pettit, V. A. Valadez, and E. M. Provin. 1997. Mobilization, cloning and sequence determination of a plasmid encoded polygalacturonase from a phytopathogenic Burkholderia (Pseudomonas) cepacia. Mol. Plant-Microbe Interact. 10:840-851[Medline].
10. Govan, J. R. W., P. H. Brown, J. Maddison, C. J. Doherty, J. W. Nelson, M. Dodd, A. P. Greening, and A. K. Webb. 1993. Evidence for transmission of Pseudomonas cepacia by social contact in cystic fibrosis. Lancet 342:15-19[CrossRef][Medline].
11. Henry, D. A., M. E. Campbell, J. J. LiPuma, and D. P. Speert. 1997. Identification of Burkholderia cepacia from patients with cystic fibrosis and use of a new selective medium. J. Clin. Microbiol. 35:614-619[Abstract].
12. Hobson, R., I. Gould, and J. Govan. 1995. Burkholderia (Pseudomonas) cepacia as a cause of brain abscesses secondary to chronic suppurative otitis media. Eur. J. Clin. Microbiol. Infect. Dis. 14:908-911[CrossRef][Medline].
13. Hutchison, M. L., I. R. Poxton, and J. R. W. Govan. 1998. Burkholderia cepacia produces a hemolysin that is capable of inducing apoptosis and degranulation of mammalian phagocytes. Infect. Immun. 66:2033-2039[Abstract/Free Full Text].
14. Johnson, W. M., S. D. Tyler, and K. R. Rozee. 1994. Linkage analysis of geographic and clinical clusters in Pseudomonas cepacia infections by multilocus enzyme electrophoresis and ribotyping. J. Clin. Microbiol. 32:924-930[Abstract/Free Full Text].
15. Larsen, G. Y., T. L. Stull, and J. L. Burns. 1993. Marked phenotypic variability in Pseudomonas cepacia isolated from a patient with cystic fibrosis. J. Clin. Microbiol. 31:788-792[Abstract/Free Full Text].
16. Lewenza, S., B. Conway, E. P. Greenberg, and P. A. Sokol. 1999. Quorum sensing in Burkholderia cepacia: identification of the LuxRI homologs CepRI. J. Bacteriol. 181:748-756[Abstract/Free Full Text].
17. LiPuma, J. J., J. E. Mortensen, S. E. Dasen, T. D. Edlind, D. V. Schidlow, J. L. Burns, and T. L. Stull. 1988. Ribotype analysis of Pseudomonas cepacia from cystic fibrosis treatment centres. J. Pediatr. 113:859-862[CrossRef][Medline].
18. LiPuma, J. J. 1998. Burkholderia cepacia---management issues and new insights. Clin. Chest Med. 19:473-486[CrossRef][Medline].
19. Mahenthiralingam, E., M. E. Campbell, D. A. Henry, and D. P. Speert. 1996. Epidemiology of Burkholderia cepacia infection in patients with cystic fibrosis: analysis by random amplified polymorphic DNA fingerprinting. J. Clin. Microbiol. 34:2914-2920[Abstract].
20. Mahenthiralingam, E., D. A. Simpson, and D. P. Speert. 1997. Identification and characterization of a novel DNA marker associated with epidemic strains of Burkholderia cepacia recovered from patients with cystic fibrosis. J. Clin. Microbiol. 35:808-816[Abstract].
21. Mahenthiralingam, E., J. Bischof, S. K. Byrne, and P. Vandamme. 1998. Molecular speciation of Burkholderia cepacia complex strains recovered from patients with cystic fibrosis. Ped. Pulmonol. 17(Suppl.):307.
22. McKenney, D., K. E. Brown, and D. G. Allison. 1995. Influence of Pseudomonas aeruginosa exoproducts on virulence factor production in Burkholderia cepacia: evidence of interspecies communication. J. Bacteriol. 177:6989-6992[Abstract/Free Full Text].
23. Millar-Jones, L., H. C. Ryley, A. Paull, and M. C. Goodchild. 1998. Transmission and prevalence of Burkholderia cepacia in Welsh cystic fibrosis patients. Respir. Med. 92:178-183[CrossRef][Medline].
24. Revets, H., P. Vandamme, A. Van Zeebroeck, K. De Boeck, M. J. Struelens, J. Verhaegen, J. P. Ursi, G. Verschraegen, H. Franckx, A. Malfroot, I. Dab, and S. Lauwers. 1996. Burkholderia (Pseudomonas) cepacia and cystic fibrosis: the epidemiology in Belgium. Acta Clin. Belg. 51:222-230[Medline].
25. Rodley, P. D., U. Römmling, and B. Tümmler. 1995. A physical genome map of the Burkholderia cepacia type strain. Mol. Microbiol. 17:57-67[Medline].
26. Sajjan, U. S., L. Sun, R. Goldstein, and J. F. Forstner. 1995. Cable (Cbl) type II pili of cystic fibrosis-associated Burkholderia (Pseudomonas) cepacia: nucleotide sequence of the cblA major subunit pilin gene and novel morphology of the assembled appendage fibers. J. Bacteriol. 177:1030-1038[Abstract/Free Full Text].
27. Simpson, I. N., J. Finlay, D. J. Winstanley, N. Dewhurst, J. W. Nelson, S. L. Butler, and J. R. W. Govan. 1994. Multi-resistant isolates possessing characteristics of both Burkholderia (Pseudomonas) cepacia and Burkholderia gladioli from patients with cystic fibrosis. J. Antimicrob. Chemother. 34:353-361[Abstract/Free Full Text].
28. Speert, D. P., M. Bond, R. C. Woodman, and J. T. Curnutte. 1994. Infection with Pseudomonas cepacia in chronic granulomatous disease: role of nonoxidative killing by neutrophils in host defence. J. Infect. Dis. 170:1524-1531[Medline].
29. Stanier, R. Y., N. J. Palleroni, and M. Doudoroff. 1966. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43:159-271[Abstract/Free Full Text].
30. Vandamme, P., B. Holmes, M. Vancanneyt, T. Coenye, B. Hoste, R. Coopman, H. Revets, S. Lauwers, M. Gillis, K. Kersters, and J. R. W. Govan. 1997. Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Int. J. Syst. Bacteriol. 47:1188-1200[Abstract/Free Full Text].
31. Van Laer, F., D. Raes, P. Vandamme, C. Lammens, J. P. Sion, C. Vrints, J. Snoeck, and H. Goossens. 1998. An outbreak of Burkholderia cepacia with septicemia on a cardiology ward. Infect. Control Hosp. Epidemiol. 19:112-113[Medline].
32. West, S. E., H. P. Schweizer, C. Dall, A. K. Sample, and L. J. Runyen-Janecky. 1994. Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa. Gene 148:81-86[CrossRef][Medline].
33. Whiteford, M. L., J. D. Wilkinson, J. H. McColl, F. M. Conlon, J. R. Michie, T. J. Evans, and J. Y. Paton. 1995. Outcome of Burkholderia (Pseudomonas) cepacia colonization in children with cystic fibrosis following a hospital outbreak. Thorax 50:1194-1198[Abstract/Free Full Text].

Journal of Clinical Microbiology, February 2000, p. 910-913, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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  • Vanlaere, E., LiPuma, J. J., Baldwin, A., Henry, D., De Brandt, E., Mahenthiralingam, E., Speert, D., Dowson, C., Vandamme, P. (2008). Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. Int. J. Syst. Evol. Microbiol. 58: 1580-1590 [Abstract] [Full Text]  
  • De Soyza, A., Silipo, A., Lanzetta, R., Govan, J. R., Molinaro, A. (2008). Review: Chemical and biological features of Burkholderia cepacia complex lipopolysaccharides. Innate Immunity 14: 127-144 [Abstract]  
  • Mellmann, A., Cloud, J., Maier, T., Keckevoet, U., Ramminger, I., Iwen, P., Dunn, J., Hall, G., Wilson, D., LaSala, P., Kostrzewa, M., Harmsen, D. (2008). Evaluation of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry in Comparison to 16S rRNA Gene Sequencing for Species Identification of Nonfermenting Bacteria. J. Clin. Microbiol. 46: 1946-1954 [Abstract] [Full Text]  
  • Aubert, D. F., Flannagan, R. S., Valvano, M. A. (2008). A Novel Sensor Kinase-Response Regulator Hybrid Controls Biofilm Formation and Type VI Secretion System Activity in Burkholderia cenocepacia. Infect. Immun. 76: 1979-1991 [Abstract] [Full Text]  
  • Zlosnik, J. E. A., Hird, T. J., Fraenkel, M. C., Moreira, L. M., Henry, D. A., Speert, D. P. (2008). Differential Mucoid Exopolysaccharide Production by Members of the Burkholderia cepacia Complex. J. Clin. Microbiol. 46: 1470-1473 [Abstract] [Full Text]  
  • Seed, K. D., Dennis, J. J. (2008). Development of Galleria mellonella as an Alternative Infection Model for the Burkholderia cepacia Complex. Infect. Immun. 76: 1267-1275 [Abstract] [Full Text]  
  • Saldias, M. S., Lamothe, J., Wu, R., Valvano, M. A. (2008). Burkholderia cenocepacia Requires the RpoN Sigma Factor for Biofilm Formation and Intracellular Trafficking within Macrophages. Infect. Immun. 76: 1059-1067 [Abstract] [Full Text]  
  • Flannagan, R. S., Valvano, M. A. (2008). Burkholderia cenocepacia requires RpoE for growth under stress conditions and delay of phagolysosomal fusion in macrophages. Microbiology 154: 643-653 [Abstract] [Full Text]  
  • Lynch, K. H., Dennis, J. J. (2008). Development of a Species-Specific fur Gene-Based Method for Identification of the Burkholderia cepacia Complex. J. Clin. Microbiol. 46: 447-455 [Abstract] [Full Text]  
  • Bernier, S. P., Nguyen, D. T., Sokol, P. A. (2008). A LysR-Type Transcriptional Regulator in Burkholderia cenocepacia Influences Colony Morphology and Virulence. Infect. Immun. 76: 38-47 [Abstract] [Full Text]  
  • Baldwin, A., Mahenthiralingam, E., Drevinek, P., Pope, C., Waine, D. J., Henry, D. A., Speert, D. P., Carter, P., Vandamme, P., LiPuma, J. J., Dowson, C. G. (2008). Elucidating Global Epidemiology of Burkholderia multivorans in Cases of Cystic Fibrosis by Multilocus Sequence Typing. J. Clin. Microbiol. 46: 290-295 [Abstract] [Full Text]  
  • Keith, K. E., Killip, L., He, P., Moran, G. R., Valvano, M. A. (2007). Burkholderia cenocepacia C5424 Produces a Pigment with Antioxidant Properties Using a Homogentisate Intermediate. J. Bacteriol. 189: 9057-9065 [Abstract] [Full Text]  
  • Mendes, R., Pizzirani-Kleiner, A. A., Araujo, W. L., Raaijmakers, J. M. (2007). Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates. Appl. Environ. Microbiol. 73: 7259-7267 [Abstract] [Full Text]  
  • Caraher, E. M., Gumulapurapu, K., Taggart, C. C., Murphy, P., McClean, S., Callaghan, M. (2007). The effect of recombinant human lactoferrin on growth and the antibiotic susceptibility of the cystic fibrosis pathogen Burkholderia cepacia complex when cultured planktonically or as biofilms. J Antimicrob Chemother 60: 546-554 [Abstract] [Full Text]  
  • Brown, A. R., Govan, J. R. W. (2007). Assessment of Fluorescent In Situ Hybridization and PCR-Based Methods for Rapid Identification of Burkholderia cepacia Complex Organisms Directly from Sputum Samples. J. Clin. Microbiol. 45: 1920-1926 [Abstract] [Full Text]  
  • Keith, K. E., Valvano, M. A. (2007). Characterization of SodC, a Periplasmic Superoxide Dismutase from Burkholderia cenocepacia. Infect. Immun. 75: 2451-2460 [Abstract] [Full Text]  
  • Malott, R. J., Sokol, P. A. (2007). Expression of the bviIR and cepIR Quorum-Sensing Systems of Burkholderia vietnamiensis. J. Bacteriol. 189: 3006-3016 [Abstract] [Full Text]  
  • Flannagan, R. S., Aubert, D., Kooi, C., Sokol, P. A., Valvano, M. A. (2007). Burkholderia cenocepacia Requires a Periplasmic HtrA Protease for Growth under Thermal and Osmotic Stress and for Survival In Vivo. Infect. Immun. 75: 1679-1689 [Abstract] [Full Text]  
  • Subsin, B., Chambers, C. E., Visser, M. B., Sokol, P. A. (2007). Identification of Genes Regulated by the cepIR Quorum-Sensing System in Burkholderia cenocepacia by High-Throughput Screening of a Random Promoter Library. J. Bacteriol. 189: 968-979 [Abstract] [Full Text]  
  • Chung, J. W., Speert, D. P. (2007). Proteomic identification and characterization of bacterial factors associated with Burkholderia cenocepacia survival in a murine host. Microbiology 153: 206-214 [Abstract] [Full Text]  
  • Maloney, K. E., Valvano, M. A. (2006). The mgtC Gene of Burkholderia cenocepacia Is Required for Growth under Magnesium Limitation Conditions and Intracellular Survival in Macrophages.. Infect. Immun. 74: 5477-5486 [Abstract] [Full Text]  
  • Kooi, C., Subsin, B., Chen, R., Pohorelic, B., Sokol, P. A. (2006). Burkholderia cenocepacia ZmpB Is a Broad-Specificity Zinc Metalloprotease Involved in Virulence. Infect. Immun. 74: 4083-4093 [Abstract] [Full Text]  
  • Markey, K. M., Glendinning, K. J., Morgan, J. A. W., Hart, C. A., Winstanley, C. (2006). Caenorhabditis elegans killing assay as an infection model to study the role of type III secretion in Burkholderia cenocepacia.. J Med Microbiol 55: 967-969 [Full Text]  
  • Loutet, S. A., Flannagan, R. S., Kooi, C., Sokol, P. A., Valvano, M. A. (2006). A Complete Lipopolysaccharide Inner Core Oligosaccharide Is Required for Resistance of Burkholderia cenocepacia to Antimicrobial Peptides and Bacterial Survival In Vivo. J. Bacteriol. 188: 2073-2080 [Abstract] [Full Text]  
  • Krejci, E., Kroppenstedt, R. M. (2006). Differentiation of Species Combined into the Burkholderia cepacia Complex and Related Taxa on the Basis of Their Fatty Acid Patterns.. J. Clin. Microbiol. 44: 1159-1164 [Abstract] [Full Text]  
  • Kenna, D. T., Yesilkaya, H., Forbes, K. J., Barcus, V. A., Vandamme, P., Govan, J. R. W. (2006). Distribution and genomic location of active insertion sequences in the Burkholderia cepacia complex. J Med Microbiol 55: 1-10 [Abstract] [Full Text]  
  • Whitby, P. W., VanWagoner, T. M., Taylor, A. A., Seale, T. W., Morton, D. J., LiPuma, J. J., Stull, T. L. (2006). Identification of an RTX determinant of Burkholderia cenocepacia J2315 by subtractive hybridization. J Med Microbiol 55: 11-21 [Abstract] [Full Text]  
  • Gingues, S., Kooi, C., Visser, M. B., Subsin, B., Sokol, P. A. (2005). Distribution and Expression of the ZmpA Metalloprotease in the Burkholderia cepacia Complex. J. Bacteriol. 187: 8247-8255 [Abstract] [Full Text]  
  • Mahenthiralingam, E, Vandamme, P (2005). Taxonomy and pathogenesis of the Burkholderia cepacia complex. Chronic Respiratory Disease 2: 209-217 [Abstract]  
  • Tomlin, K. L., Malott, R. J., Ramage, G., Storey, D. G., Sokol, P. A., Ceri, H. (2005). Quorum-Sensing Mutations Affect Attachment and Stability of Burkholderia cenocepacia Biofilms. Appl. Environ. Microbiol. 71: 5208-5218 [Abstract] [Full Text]  
  • Baldwin, A., Mahenthiralingam, E., Thickett, K. M., Honeybourne, D., Maiden, M. C. J., Govan, J. R., Speert, D. P., LiPuma, J. J., Vandamme, P., Dowson, C. G. (2005). Multilocus Sequence Typing Scheme That Provides Both Species and Strain Differentiation for the Burkholderia cepacia Complex. J. Clin. Microbiol. 43: 4665-4673 [Abstract] [Full Text]  
  • Bernier, S. P., Sokol, P. A. (2005). Use of Suppression-Subtractive Hybridization To Identify Genes in the Burkholderia cepacia Complex That Are Unique to Burkholderia cenocepacia. J. Bacteriol. 187: 5278-5291 [Abstract] [Full Text]  
  • Payne, G. W., Vandamme, P., Morgan, S. H., LiPuma, J. J., Coenye, T., Weightman, A. J., Jones, T. H., Mahenthiralingam, E. (2005). Development of a recA Gene-Based Identification Approach for the Entire Burkholderia Genus. Appl. Environ. Microbiol. 71: 3917-3927 [Abstract] [Full Text]  
  • Lefebre, M. D., Flannagan, R. S., Valvano, M. A. (2005). A minor catalase/peroxidase from Burkholderia cenocepacia is required for normal aconitase activity. Microbiology 151: 1975-1985 [Abstract] [Full Text]  
  • Ramette, A., LiPuma, J. J., Tiedje, J. M. (2005). Species Abundance and Diversity of Burkholderia cepacia Complex in the Environment. Appl. Environ. Microbiol. 71: 1193-1201 [Abstract] [Full Text]  
  • Ortega, X., Hunt, T. A., Loutet, S., Vinion-Dubiel, A. D., Datta, A., Choudhury, B., Goldberg, J. B., Carlson, R., Valvano, M. A. (2005). Reconstitution of O-Specific Lipopolysaccharide Expression in Burkholderia cenocepacia Strain J2315, Which Is Associated with Transmissible Infections in Patients with Cystic Fibrosis. J. Bacteriol. 187: 1324-1333 [Abstract] [Full Text]  
  • Jones, A M, Dodd, M E, Govan, J R W, Barcus, V, Doherty, C J, Morris, J, Webb, A K (2004). Burkholderia cenocepacia and Burkholderia multivorans: influence on survival in cystic fibrosis. Thorax 59: 948-951 [Abstract] [Full Text]  
  • Engledow, A. S., Medrano, E. G., Mahenthiralingam, E., LiPuma, J. J., Gonzalez, C. F. (2004). Involvement of a Plasmid-Encoded Type IV Secretion System in the Plant Tissue Watersoaking Phenotype of Burkholderia cenocepacia. J. Bacteriol. 186: 6015-6024 [Abstract] [Full Text]  
  • Vinion-Dubiel, A. D., Spilker, T., Dean, C. R., Monteil, H., LiPuma, J. J., Goldberg, J. B. (2004). Correlation of wbiI Genotype, Serotype, and Isolate Source within Species of the Burkholderia cepacia Complex. J. Clin. Microbiol. 42: 4121-4126 [Abstract] [Full Text]  
  • Hunt, T. A., Kooi, C., Sokol, P. A., Valvano, M. A. (2004). Identification of Burkholderia cenocepacia Genes Required for Bacterial Survival In Vivo. Infect. Immun. 72: 4010-4022 [Abstract] [Full Text]  
  • De Soyza, A., Ellis, C. D., Khan, C. M. A., Corris, P. A., de Hormaeche, R. D. (2004). Burkholderia cenocepacia Lipopolysaccharide, Lipid A, and Proinflammatory Activity. Am. J. Respir. Crit. Care Med. 170: 70-77 [Abstract] [Full Text]  
  • DeShazer, D. (2004). Genomic Diversity of Burkholderia pseudomallei Clinical Isolates: Subtractive Hybridization Reveals a Burkholderia mallei-Specific Prophage in B. pseudomallei 1026b. J. Bacteriol. 186: 3938-3950 [Abstract] [Full Text]  
  • Visser, M. B., Majumdar, S., Hani, E., Sokol, P. A. (2004). Importance of the Ornibactin and Pyochelin Siderophore Transport Systems in Burkholderia cenocepacia Lung Infections. Infect. Immun. 72: 2850-2857 [Abstract] [Full Text]  
  • Plesa, M., Kholti, A., Vermis, K., Vandamme, P., Panagea, S., Winstanley, C., Cornelis, P. (2004). Conservation of the opcL gene encoding the peptidoglycan-associated outer-membrane lipoprotein among representatives of the Burkholderia cepacia complex. J Med Microbiol 53: 389-398 [Abstract] [Full Text]  
  • Weaver, V. B., Kolter, R. (2004). Burkholderia spp. Alter Pseudomonas aeruginosa Physiology through Iron Sequestration. J. Bacteriol. 186: 2376-2384 [Abstract] [Full Text]  
  • Baldwin, A., Sokol, P. A., Parkhill, J., Mahenthiralingam, E. (2004). The Burkholderia cepacia Epidemic Strain Marker Is Part of a Novel Genomic Island Encoding Both Virulence and Metabolism-Associated Genes in Burkholderia cenocepacia. Infect. Immun. 72: 1537-1547 [Abstract] [Full Text]  
  • Sokol, P. A., Sajjan, U., Visser, M. B., Gingues, S., Forstner, J., Kooi, C. (2003). The CepIR quorum-sensing system contributes to the virulence of Burkholderia cenocepacia respiratory infections. Microbiology 149: 3649-3658 [Abstract] [Full Text]  
  • Hearn, E. M., Dennis, J. J., Gray, M. R., Foght, J. M. (2003). Identification and Characterization of the emhABC Efflux System for Polycyclic Aromatic Hydrocarbons in Pseudomonas fluorescens cLP6a. J. Bacteriol. 185: 6233-6240 [Abstract] [Full Text]  
  • Aguilar, C., Friscina, A., Devescovi, G., Kojic, M., Venturi, V. (2003). Identification of Quorum-Sensing-Regulated Genes of Burkholderia cepacia. J. Bacteriol. 185: 6456-6462 [Abstract] [Full Text]  
  • Bernier, S. P., Silo-Suh, L., Woods, D. E., Ohman, D. E., Sokol, P. A. (2003). Comparative Analysis of Plant and Animal Models for Characterization of Burkholderia cepacia Virulence. Infect. Immun. 71: 5306-5313 [Abstract] [Full Text]  
  • Detsika, M. G., Corkill, J. E., Magalhaes, M., Glendinning, K. J., Hart, C. A., Winstanley, C. (2003). Molecular Typing of, and Distribution of Genetic Markers among, Burkholderia cepacia Complex Isolates from Brazil. J. Clin. Microbiol. 41: 4148-4153 [Abstract] [Full Text]  
  • Corbett, C. R., Burtnick, M. N., Kooi, C., Woods, D. E., Sokol, P. A. (2003). An extracellular zinc metalloprotease gene of Burkholderia cepacia. Microbiology 149: 2263-2271 [Abstract] [Full Text]  
  • Coenye, T., Vandamme, P., LiPuma, J. J., Govan, J. R. W., Mahenthiralingam, E. (2003). Updated Version of the Burkholderia cepacia Complex Experimental Strain Panel. J. Clin. Microbiol. 41: 2797-2798 [Full Text]  
  • Langley, R., Kenna, D. T., Vandamme, P., Ure, R., Govan, J. R. W. (2003). Lysogeny and bacteriophage host range within the Burkholderia cepacia complex. J Med Microbiol 52: 483-490 [Abstract] [Full Text]  
  • Punj, V., Sharma, R., Zaborina, O., Chakrabarty, A. M. (2003). Energy-Generating Enzymes of Burkholderia cepacia and Their Interactions with Macrophages. J. Bacteriol. 185: 3167-3178 [Abstract] [Full Text]  
  • Sahly, H., Schubert, S., Harder, J., Rautenberg, P., Ullmann, U., Schroder, J., Podschun, R. (2003). Burkholderia Is Highly Resistant to Human Beta-Defensin 3. Antimicrob. Agents Chemother. 47: 1739-1741 [Abstract] [Full Text]  
  • Smalley, J. W., Charalabous, P., Hart, C. A., Silver, J. (2003). Transmissible Burkholderia cepacia genomovar IIIa strains bind and convert monomeric iron(III) protoporphyrin IX into the {micro}-oxo oligomeric form. Microbiology 149: 843-853 [Abstract] [Full Text]  
  • Chung, J. W., Altman, E., Beveridge, T. J., Speert, D. P. (2003). Colonial Morphology of Burkholderia cepacia Complex Genomovar III: Implications in Exopolysaccharide Production, Pilus Expression, and Persistence in the Mouse. Infect. Immun. 71: 904-909 [Abstract] [Full Text]  
  • Sajjan, U., Liu, L., Lu, A., Spilker, T., Forstner, J., LiPuma, J. J. (2002). Lack of cable pili expression by cblA-containing Burkholderia cepacia complex. Microbiology 148: 3477-3484 [Abstract] [Full Text]  
  • VERMIS, K., COENYE, T., MAHENTHIRALINGAM, E., NELIS, H. J., VANDAMME, P. (2002). Evaluation of species-specific recA-based PCR tests for genomovar level identification within the Burkholderia cepacia complex. J Med Microbiol 51: 937-940 [Abstract] [Full Text]  
  • Conway, B.-A. D., Venu, V., Speert, D. P. (2002). Biofilm Formation and Acyl Homoserine Lactone Production in the Burkholderia cepacia Complex. J. Bacteriol. 184: 5678-5685 [Abstract] [Full Text]  
  • Drevinek, P., Hrbackova, H., Cinek, O., Bartosova, J., Nyc, O., Nemec, A., Pohunek, P. (2002). Direct PCR Detection of Burkholderia cepacia Complex and Identification of Its Genomovars by Using Sputum as Source of DNA. J. Clin. Microbiol. 40: 3485-3488 [Abstract] [Full Text]  
  • Nzula, S., Vandamme, P., Govan, J. R. W. (2002). Influence of taxonomic status on the in vitro antimicrobial susceptibility of the Burkholderia cepacia complex. J Antimicrob Chemother 50: 265-269 [Abstract] [Full Text]  
  • Woods, D. E., Jeddeloh, J. A., Fritz, D. L., DeShazer, D. (2002). Burkholderia thailandensis E125 Harbors a Temperate Bacteriophage Specific for Burkholderia mallei. J. Bacteriol. 184: 4003-4017 [Abstract] [Full Text]  
  • MAHENTHIRALINGAM, E., BALDWIN, A., VANDAMME, P. (2002). Burkholderia cepacia complex infection in patients with cystic fibrosis. J Med Microbiol 51: 533-538 [Abstract] [Full Text]  
  • Chu, K. K., Davidson, D. J., Halsey, T. K., Chung, J. W., Speert, D. P. (2002). Differential Persistence among Genomovars of the Burkholderia cepacia Complex in a Murine Model of Pulmonary Infection. Infect. Immun. 70: 2715-2720 [Abstract] [Full Text]  
  • Bevivino, A., Dalmastri, C., Tabacchioni, S., Chiarini, L., Belli, M. L., Piana, S., Materazzo, A., Vandamme, P., Manno, G. (2002). Burkholderia cepacia Complex Bacteria from Clinical and Environmental Sources in Italy: Genomovar Status and Distribution of Traits Related to Virulence and Transmissibility. J. Clin. Microbiol. 40: 846-851 [Abstract] [Full Text]  
  • Cieri, M. V., Mayer-Hamblett, N., Griffith, A., Burns, J. L. (2002). Correlation between an In Vitro Invasion Assay and a Murine Model of Burkholderia cepacia Lung Infection. Infect. Immun. 70: 1081-1086 [Abstract] [Full Text]  
  • Coenye, T., Vandamme, P., Govan, J. R. W., LiPuma, J. J. (2001). Taxonomy and Identification of the Burkholderia cepacia Complex. J. Clin. Microbiol. 39: 3427-3436 [Full Text]  
  • Sajjan, U., Thanassoulis, G., Cherapanov, V., Lu, A., Sjolin, C., Steer, B., Wu, Y. J., Rotstein, O. D., Kent, G., McKerlie, C., Forstner, J., Downey, G. P. (2001). Enhanced Susceptibility to Pulmonary Infection with Burkholderia cepacia in Cftr{-}/{-} Mice. Infect. Immun. 69: 5138-5150 [Abstract] [Full Text]  
  • Agodi, A., Mahenthiralingam, E., Barchitta, M., Giannino, V., Sciacca, A., Stefani, S. (2001). Burkholderia cepacia Complex Infection in Italian Patients with Cystic Fibrosis: Prevalence, Epidemiology, and Genomovar Status. J. Clin. Microbiol. 39: 2891-2896 [Abstract] [Full Text]  
  • WINSTANLEY, C., DETSIKA, M. G., GLENDINNING, K. J., PARSONS, Y. N., HART, C. A. (2001). Flagellin gene PCR-RFLP analysis of a panel of strains from the Burkholderia cepacia complex. J Med Microbiol 50: 728-731 [Abstract] [Full Text]  
  • Lutter, E., Lewenza, S., Dennis, J. J., Visser, M. B., Sokol, P. A. (2001). Distribution of Quorum-Sensing Genes in the Burkholderia cepacia Complex. Infect. Immun. 69: 4661-4666 [Abstract] [Full Text]  
  • LiPUMA, J. J., SPILKER, T., GILL, L. H., CAMPBELL, P. W. III, LIU, L., MAHENTHIRALINGAM, E. (2001). Disproportionate Distribution of Burkholderia cepacia Complex Species and Transmissibility Markers in Cystic Fibrosis. Am. J. Respir. Crit. Care Med. 164: 92-96 [Abstract] [Full Text]  
  • CHIU, C-H., OSTRY, A., SPEERT, D.P. (2001). Invasion of murine respiratory epithelial cells in vivo by Burkholderia cepacia. J Med Microbiol 50: 594-601 [Abstract] [Full Text]  
  • Smalley, J. W., Charalabous, P., Birss, A. J., Hart, C. A. (2001). Detection of Heme-Binding Proteins in Epidemic Strains of Burkholderia cepacia. CVI 8: 509-514 [Abstract] [Full Text]  
  • Lewenza, S., Sokol, P. A. (2001). Regulation of Ornibactin Biosynthesis and N-Acyl-L-Homoserine Lactone Production by CepR in Burkholderia cepacia. J. Bacteriol. 183: 2212-2218 [Abstract] [Full Text]  
  • Berriatua, E., Ziluaga, I., Miguel-Virto, C., Uribarren, P., Juste, R., Laevens, S., Vandamme, P., Govan, J. R. W. (2001). Outbreak of Subclinical Mastitis in a Flock of Dairy Sheep Associated with Burkholderia cepacia Complex Infection. J. Clin. Microbiol. 39: 990-994 [Abstract] [Full Text]  
  • Henry, D. A., Mahenthiralingam, E., Vandamme, P., Coenye, T., Speert, D. P. (2001). Phenotypic Methods for Determining Genomovar Status of the Burkholderia cepacia Complex. J. Clin. Microbiol. 39: 1073-1078 [Abstract] [Full Text]  
  • Jones, A.M., Dodd, M.E., Webb, A.K. (2001). Burkholderia cepacia: current clinical issues, environmental controversies and ethical dilemmas. Eur Respir J 17: 295-301 [Abstract] [Full Text]  
  • Sokol, P. A., Darling, P., Lewenza, S., Corbett, C. R., Kooi, C. D. (2000). Identification of a Siderophore Receptor Required for Ferric Ornibactin Uptake in Burkholderia cepacia. Infect. Immun. 68: 6554-6560 [Abstract] [Full Text]  
  • Mahenthiralingam, E., Bischof, J., Byrne, S. K., Radomski, C., Davies, J. E., Av-Gay, Y., Vandamme, P. (2000). DNA-Based Diagnostic Approaches for Identification of Burkholderia cepacia Complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia Genomovars I and III. J. Clin. Microbiol. 38: 3165-3173 [Abstract] [Full Text]  

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