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Journal of Clinical Microbiology, June 2000, p. 2170-2173, Vol. 38, No. 6
Department of Microbiology, SD Laboratory
Services, Sunnybrook and Women's College Health Sciences Centre,
and the University of Toronto, Toronto, Ontario M4N 3M5, Canada
Received 24 November 1999/Returned for modification 10 February
2000/Accepted 6 March 2000
The probe-based Velogene Rapid MRSA Identification Assay (ID
Biomedical Corp., Vancouver, British Columbia, Canada) and the latex agglutination MRSA-Screen (Denka Seiken Co., Tokyo, Japan) were
evaluated for their ability to identify methicillin-resistant Staphylococcus aureus (MRSA) and to distinguish strains of
MRSA from borderline oxacillin-resistant S. aureus (BORSA;
mecA-negative, oxacillin MICs of 2 to 8 µg/ml). The
Velogene is a 90-min assay using a chimeric probe to detect the
mecA gene. MRSA-Screen is a 15-min latex agglutination test
with penicillin-binding protein 2a antibody-sensitized latex particles.
We compared these assays with the BBL Crystal MRSA ID System (Becton
Dickinson, Cockeysville, Md.) and with PCR for mecA gene
detection. A total of 397 clinical isolates of S. aureus
were tested, consisting of 164 methicillin-susceptible strains, 197 MRSA strains, and 37 BORSA strains. All assays performed well for the
identification of MRSA with sensitivities and specificities for
Velogene, MRSA-Screen, and BBL Crystal MRSA ID of 98.5 and 100%, 98.5 and 100%, and 98.5 and 98%, respectively. Three MRSA strains were not
correctly identified by each of the Velogene and MRSA-Screen assays,
but repeat testing with a larger inoculum resolved the discrepancies.
The BBL Crystal MRSA ID test misclassified four BORSA strains as MRSA.
Both the Velogene and the MRSA-Screen assays are easy to perform, can
accurately differentiate BORSA isolates from MRSA isolates, and
provide a rapid alternative for the detection of methicillin resistance
in S. aureus in clinical laboratories, especially when
mecA PCR gene detection is unavailable.
Methicillin-resistant
Staphylococcus aureus (MRSA) has become increasingly
prevalent worldwide. In the United States and in some European
countries, MRSA accounts for 10 to 40% of all S. aureus
isolates (16, 26). Increased surveillance, including screening of high-risk patients, has been recognized as an important component of effective infection control programs to limit the spread
of MRSA in hospitals. Therefore, rapid and accurate identification of
MRSA is essential. Traditional antimicrobial susceptibility test
methods such as disk diffusion or broth microdilution require at least
24 h to perform. In addition, problems in the laboratory identification of MRSA may occur due to low-level expression of oxacillin resistance in certain strains of S. aureus.
Difficulties in the differentiation of MRSA from borderline
oxacillin-resistant S. aureus (BORSA) strains may
also occur (8, 10).
Methicillin resistance in S. aureus is mediated by the
production of an altered penicillin-binding protein, PBP 2a
(5). The mec gene complex regulates the
production of PBP 2a. Detection of the mecA gene or of PBP
2a appears to most accurately detect methicillin resistance in S. aureus (1, 5, 6, 15, 21, 22). However, the use of these
assays is largely restricted to reference centers, and they are not
currently utilized by most routine diagnostic laboratories.
Bekkaoui et al. (2) recently described the development of a
2-h assay utilizing cycling probe technology with a DNA-RNA-DNA chimeric probe designed to detect the mecA gene in
S. aureus. The resulting Velogene Rapid MRSA
Identification Assay (ID Biomedical Corp., Vancouver, British Columbia,
Canada) is a colorimetric enzyme immunoassay (EIA) utilizing a
fluorescein-labeled mecA probe. This subtractive assay uses
a streptavidin-coated 96-well microtiter plate format, and the
detection of uncut probe from mecA negative strains results
in the development of a blue color, whereas mecA-positive
strains result in a colorless reaction.
In 1998, Nakatomi and Sugiyama (13) reported on the
development of a simple test for the detection of the mecA
gene product, PBP 2a. The resulting commercially available assay, the
MRSA-Screen (Denka Seiken Co., Tokyo, Japan), is a 15-min slide
latex agglutination test using latex particles sensitized with a
monoclonal antibody against PBP 2a (4).
In this study, we evaluated these two new tests for the detection of
methicillin resistance in S. aureus. The assays were compared to standard methods of susceptibility testing and to another
commercially available kit, the BBL Crystal MRSA ID System (Becton
Dickinson, Cockeysville, Md.) (9). Detection of the mecA gene by PCR was used as the "gold standard" in this evaluation.
Clinical specimens.
A total of 397 well-characterized
clinical isolates of S. aureus were selected for
testing, consisting of 163 methicillin-susceptible S. aureus
(MSSA) strains (oxacillin MIC, Velogene Rapid MRSA Identification Assay.
Testing of
isolates using the Velogene assay was performed in accordance with the
manufacturer's instructions. Briefly, a 1-µl loopful of growth from
a blood agar plate was suspended in 50 µl of lysis buffer and
incubated at 55°C for 20 min. The suspension was then incubated in a
dry bath at 95°C for 5 min. A 50-µl aliquot of cycling reagent was
added, and the suspension was incubated at 55°C for a further 25 min.
Cycle stop reagent was added, and the suspension was transferred to
streptavidin-coated microtiter wells incubated at room temperature for
10 min. After two washes, detection substrate reagent was added. After
color development a detection stop reagent was added. The development
of a blue color was indicative of a methicillin-susceptible isolate
(mecA negative); a colorless reaction indicated the presence
of a methicillin-resistant strain (mecA positive). The test
results could be determined spectrophotometrically or by visual
inspection. For this evaluation, we used visual inspection for
determination of the assay's sensitivity and specificity.
MRSA-Screen.
The MRSA-Screen test was performed according to
the manufacturer's instructions. A 1-µl loopful of the test isolate
was emulsified in 4 drops of an extraction reagent and boiled for 3 min. This suspension was then allowed to cool to room temperature, and
1 drop of a second extraction reagent was added and mixed. This suspension was centrifuged at 1,500 × g for 5 min. A
50-µl aliquot of the supernatant was added to each of two circles on
a disposable test card and mixed with 1 drop of the anti-PBP 2a
monoclonal antibody sensitized latex and 1 drop of the negative control
latex, respectively. The samples were then mixed for 3 min on a shaker, and agglutination was observed visually.
BBL Crystal MRSA ID.
Testing with the BBL Crystal MRSA ID
system was performed according to the manufacturer's instructions. The
inoculated tray was incubated at 35°C for 4 h, and fluorescence
in wells was observed by illuminating the panel with long-wave UV light.
Oxacillin agar screen and oxacillin susceptibility testing.
Antimicrobial susceptibility testing of isolates using an oxacillin
agar screen plate (Mueller-Hinton agar supplemented with 4% NaCl and 6 µg of oxacillin per ml) and by microbroth dilution were performed in
accordance with National Committee for Clinical Laboratory Standards
(NCCLS) guidelines (14).
Multiplex PCR.
PCR was performed for the simultaneous
detection of mecA (12) and nucA
(3). The nucA gene is responsible for the
production of thermostable nuclease and was included in the multiplex
PCR assay in order to confirm that the isolates were indeed S. aureus and not other staphylococcal species. Bacterial DNA was
extracted using two to three colonies of a test organism grown on a 5%
sheep blood agar plate and then boiled for 10 min in 100 µl of Triton X-100 lysis buffer (100 mM NaCl, 10 mM Tris-HCl [pH 8], 1 mM EDTA [pH 9], and 1% Triton X-100) (20). The suspension was
cooled at room temperature for 5 min and centrifuged at 14,000 rpm for 1 min. Next, 1 µl of the supernatant was used as the template. PCR
was performed in a 25-µl volume, with 1× PCR buffer containing 10 mM
Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, 200 µM
concentrations of each deoxynucleoside triphosphate, 2.5 U of
Taq polymerase, and 0.2 µM concentrations of each primer.
Thermocycling conditions in a GeneAmp 9600 thermocycler (PE Biosystems,
Mississauga, Ontario, Canada) were as follows: 94°C for 2 min,
followed by 30 cycles of 94°C for 1 s and 55°C for 15 s,
with a final 10-min extension at 72°C. The primer sequences for
mecA and nucA are shown in Table 1. The control organisms included
S. aureus ATCC 43300, S. aureus ATCC 25923, and
S. epidermidis ATCC 12228. Electrophoresis at 100 V for 40 min was performed to separate the products on 1% 1× TBE (8.9 M Tris,
8.9 M boric acid, and 0.2 M EDTA) agarose gels. Gels were stained with
ethidium bromide and photographed under UV illumination.
0095-1137/00/$04.00+0
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Evaluation of Three Rapid Methods for Detection of
Methicillin Resistance in Staphylococcus aureus
ABSTRACT
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
INTRODUCTION
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
MATERIALS AND METHODS
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
1 µg/ml; mecA negative), 197 MRSA strains (oxacillin MIC, 
4 µg/ml; mecA
positive), and 37 BORSA strains (oxacillin MIC, 2 to 8 µg/ml;
mecA negative). The isolates had been typed by pulsed-field
gel electrophoresis and were shown to have represented distinct
genotypes. All isolates were stored frozen in buffered glycerol at
70°C and were subcultured twice onto Trypticase soy agar
supplemented with 5% sheep blood prior to testing. All isolates were
subjected to "blinded" testing with the Velogene assay, the
MRSA-Screen test kit, the BBL Crystal MRSA ID test, the oxacillin agar
screen plate test (14), and determination of oxacillin MICs
by broth microdilution testing (14). Control strains used
for all assays included the MRSA strains ATCC 33592 and ATCC 43300 and
MSSA strain ATCC 29213.
TABLE 1.
Primers used for multiplex PCR for the identification
of methicillin resistance in S. aureus
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RESULTS |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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The results of testing with the Velogene Rapid MRSA
Identification, MRSA-Screen, and BBL Crystal MRSA ID assays are
summarized in Table 2. Retesting a subset
of 60 isolates with these assays yielded identical results. Discrepant
test results obtained with these assays are summarized in Table
3.
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The Velogene Rapid MRSA Identification Assay was able to accurately detect methicillin resistance in almost all strains (sensitivity, 98.5%), including those with low-level resistance (oxacillin MICs, 4 to 8 µg/ml). There were no false-positive reactions when testing MSSA or BORSA strains (specificity, 100%). However, with visual interpretation of test results, three strains of MRSA were initially identified as methicillin susceptible (Table 3). These three strains were very "sticky" in consistency and were difficult to scrape off the plate. This created problems when emulsifying the organisms in the lysis buffer provided. Two of these three strains were identified as MRSA strains when the test results were read by spectrophotometer. Repeat testing of these two strains with a larger inoculum gave correct results both visually and spectrophotometrically. Use of the larger inoculum did not decrease the specificity of the assay.
The MRSA-Screen latex agglutination assay also had excellent sensitivity (98.5%) and specificity (100%) for the detection of methicillin resistance in S. aureus. However, methicillin resistance was not detected in three isolates (Table 3). Upon retesting these isolates with a larger inoculum, all three were found to agglutinate with the anti-PBP 2a-sensitized latex. No false-positive reactions were observed with a larger inoculum, and no autoagglutination was observed in the control latex reagent.
The BBL Crystal MRSA ID System performed well for the detection of MRSA
strains, although some BORSA isolates and MRSA strains with oxacillin
MICs of 8 µg/ml were misidentified (Table 3). The sensitivity of
this assay was 98.5%, and the specificity was 98%.
The oxacillin agar screen plate performed well for the detection of MRSA strains, missing only two, both with oxacillin MICs of 4 µg/ml (sensitivity, 99%). Growth of all of the MSSA strains and of eight BORSA strains was suppressed on the screen plate; 29 BORSA strains did grow on the oxacillin screen plate (specificity, 85.5%).
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DISCUSSION |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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It is known that many strains of MRSA demonstrate heterogeneous expression of oxacillin resistance (5, 7). As a result, laboratory methods have been developed to enhance the expression of resistance in staphylococci, including the supplementation of media with NaCl and prolonging the incubation period to 24 h (7). The use of the oxacillin agar screen plate containing 6 µg of oxacillin per ml, as recommended by the NCCLS (14), has been very useful for identifying MRSA, although many BORSA strains will also grow on this medium. Rapid commercially available methods of detecting methicillin resistance in staphylococci, such as the BBL Crystal MRSA ID test kit, have been developed and, as in the present study, these methods have been found to be useful (9, 17). Nevertheless, difficulties exist in accurately identifying MRSA and in differentiating these strains from BORSA strains (18, 19, 23). In this study, we wished to determine the accuracy of two new rapid diagnostic tests for the detection of methicillin resistance in S. aureus. A large number of BORSA isolates was included in this evaluation in order to challenge the assays.
The Velogene Rapid MRSA Identification Assay was rapid and easy to perform, providing results in approximately 90 min. This test compares favorably with conventional susceptibility test methods and provides more rapid results. Test results can be interpreted visually or by using a spectrophotometer. Problems were occasionally encountered with certain strains of S. aureus with a very "sticky" or "waxy" consistency. Since these strains were difficult to pick off an agar plate, a one-loopful inoculum (approximately two to five colonies), as recommended by the manufacturer, may not provide sufficient target for the test assay. As a result, a small number of MRSA strains were not correctly identified. However, improvements to the assay's sensitivity could be achieved by using a heavier inoculum, without affecting the excellent specificity of the assay. Because of its microwell-EIA detection format, the test can easily be adopted for testing of multiple isolates simultaneously, and 20 isolates can easily be tested in less than 2 h.
The MRSA-Screen accurately detected the mecA gene product, PBP 2a, in almost all of the test strains of MRSA, as previously reported by other investigators (4, 24, 25). False-negative results occurred with three of the MRSA isolates with oxacillin MICs of either 4 or 8 µg/ml. Similar results had been reported by van Griethuysen et al. (24), who speculated that certain MRSA strains with lower MICs may produce smaller amounts of PBP 2a. The control strain, S. aureus ATCC 43300 (oxacillin MIC of 8 µg/ml) (11), also gave a negative result with an inoculum of only 1 µl (a loopful), as recommended by the manufacturer at the time of this evaluation. Using a larger inoculum of approximately 50 colonies (a large, "heaping" loopful) resulted in improved sensitivity of the assay without loss of specificity. The manufacturer has recently changed its recommendations, indicating the need for use of a higher inoculum or more time for agglutination. In another recent report (25), MRSA strains that initially failed to agglutinate with the MRSA-Screen assay were retested after incubation in the presence of a 5-µg methicillin disk in order to increase the level of PBP 2a expression. However, this would add to the total amount of time required for laboratory confirmation of MRSA. The MRSA-Screen assay is simple to perform, highly sensitive and specific, and can easily be incorporated into any clinical diagnostic laboratory.
Attempts to use genotypic methods for the identification of MRSA have generally been limited to specialized reference laboratories. With the introduction of newer assays, such as the Velogene Rapid MRSA Identification Assay and the MRSA-Screen, diagnostic laboratories will have better tools at their disposal for rapid and accurate detection of methicillin resistance in S. aureus. The time and cost savings that are realized with these newer, genotype-based assays will allow clinicians and infection control practitioners to more effectively manage patients and control the spread of MRSA. These newer tests show great promise in providing rapid, sensitive and specific alternatives for clinical laboratories where PCR or DNA hybridization for the mecA gene is not readily available.
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FOOTNOTES |
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* Corresponding author. Mailing address: Department of Microbiology, Sunnybrook and Women's College Health Sciences Centre, B121-2075 Bayview Ave., North York, Ontario, Canada M4N 3M5. Phone: 416-480-4242. Fax: 416-480-6845. E-mail: llouie{at}srcl.sunnybrook.utoronto.ca.
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REFERENCES |
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References
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Journal of Clinical Microbiology, June 2000, p. 2170-2173, Vol. 38, No. 6
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Copyright © 2000, American Society for Microbiology. All rights reserved.
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[Abstract] [Full Text] -
Louie, L., Goodfellow, J., Mathieu, P., Glatt, A., Louie, M., Simor, A. E.
(2002). Rapid Detection of Methicillin-Resistant Staphylococci from Blood Culture Bottles by Using a Multiplex PCR Assay. J. Clin. Microbiol.
40: 2786-2790
[Abstract] [Full Text] -
Merlino, J., Watson, J., Rose, B., Beard-Pegler, M., Gottlieb, T., Bradbury, R., Harbour, C.
(2002). Detection and expression of methicillin/oxacillin resistance in multidrug-resistant and non-multidrug-resistant Staphylococcus aureus in Central Sydney, Australia. J Antimicrob Chemother
49: 793-801
[Abstract] [Full Text] -
Jonas, D., Speck, M., Daschner, F. D., Grundmann, H.
(2002). Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs. J. Clin. Microbiol.
40: 1821-1823
[Abstract] [Full Text] -
Sakoulas, G., Gold, H. S., Venkataraman, L., DeGirolami, P. C., Eliopoulos, G. M., Qian, Q.
(2001). Methicillin-Resistant Staphylococcus aureus: Comparison of Susceptibility Testing Methods and Analysis of mecA-Positive Susceptible Strains. J. Clin. Microbiol.
39: 3946-3951
[Abstract] [Full Text] -
Louie, L., Majury, A., Goodfellow, J., Louie, M., Simor, A. E.
(2001). Evaluation of a Latex Agglutination Test (MRSA-Screen) for Detection of Oxacillin Resistance in Coagulase-Negative Staphylococci. J. Clin. Microbiol.
39: 4149-4151
[Abstract] [Full Text] -
Yamazumi, T., Furuta, I., Diekema, D. J., Pfaller, M. A., Jones, R. N.
(2001). Comparison of the Vitek Gram-Positive Susceptibility 106 Card, the MRSA-Screen Latex Agglutination Test, and mecA Analysis for Detecting Oxacillin Resistance in a Geographically Diverse Collection of Clinical Isolates of Coagulase-Negative Staphylococci. J. Clin. Microbiol.
39: 3633-3636
[Abstract] [Full Text] -
Horstkotte, M. A., Knobloch, J. K.-M., Rohde, H., Mack, D.
(2001). Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci by a Penicillin-Binding Protein 2a-Specific Latex Agglutination Test. J. Clin. Microbiol.
39: 3700-3702
[Abstract] [Full Text] -
Swenson, J. M., Williams, P. P., Killgore, G., O'Hara, C. M., Tenover, F. C.
(2001). Performance of Eight Methods, Including Two New Rapid Methods, for Detection of Oxacillin Resistance in a Challenge Set of Staphylococcus aureus Organisms. J. Clin. Microbiol.
39: 3785-3788
[Abstract] [Full Text] -
Simor, A. E., Goodfellow, J., Louie, L., Louie, M.
(2001). Evaluation of a New Medium, Oxacillin Resistance Screening Agar Base, for the Detection of Methicillin-Resistant Staphylococcus aureus from Clinical Specimens. J. Clin. Microbiol.
39: 3422-3422
[Full Text] -
Yamazumi, T., Marshall, S. A., Wilke, W. W., Diekema, D. J., Pfaller, M. A., Jones, R. N.
(2001). Comparison of the Vitek Gram-Positive Susceptibility 106 Card and the MRSA-Screen Latex Agglutination Test for Determining Oxacillin Resistance in Clinical Bloodstream Isolates of Staphylococcus aureus. J. Clin. Microbiol.
39: 53-56
[Abstract] [Full Text]
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