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Journal of Clinical Microbiology, October 2002, p. 3874-3876, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3874-3876.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

CASE REPORT

Japanese Spotted Fever Involving the Central Nervous System: Two Case Reports and a Literature Review

Manabu Araki,1,{dagger} Katsuya Takatsuka,1 Junichiro Kawamura,1* and Yuko Kanno2

Neurology,1 Dermatology, Kobe City General Hospital, Hyogo, Japan2

Received 15 March 2002/ Accepted 17 July 2002

ABSTRACT

Japanese spotted fever (JSF), first reported in 1984, is a rickettsial disease caused by Rickettsia japonica. Until now, affliction of the central nervous system has been rarely reported. Here we report two cases of JSF associated with a central nervous system disorder such as meningoencephalitis.

CASE REPORTS

Case 1. A 58-year-old man mowed brushy vegetation on 15 August 1992. Eight days later, he developed a high fever and exanthemata on his upper extremities and trunk. He was admitted to a local hospital on 28 August 1992.

On admission he was afebrile. His extremities and trunk were covered with erythematous exanthemata, parts of which were petechial. His skin was also icteric. An eschar was detected on his left thigh. Neither lymphadenopathy nor hepatosplenomegaly was noted. His neck was stiff, and he was obtunded, with occasional mumbling, but otherwise his neurological examination showed no abnormalities.

Laboratory studies revealed leukocytosis, hepatic dysfunction, and bilirubinemia. His C-reactive protein (CRP) level was 6.3 mg/dl (versus a normal range of 0 to 0.5 mg/dl). Urinalysis showed bilirubinuria without hematuria and pyuria. Chest films and a computed tomography (CT) scan of the head were normal. His cerebrospinal fluid (CSF) contained 46 cells/mm3, which consisted of 30 monocytes and 16 neutrophils/mm3, 107 mg of sugar per dl, and 125 mg of protein per dl.

Intravenous administrations of various antibiotics including cefmetazole, clindamycin, aztreonam, and imipenem-cilastatin were ineffective. His CRP rose to a peak of 19.0 mg/dl. Since the exanthemata and eschar suggested rickettsial infection, 10 days after the onset of clinical symptoms, minocycline hydrochloride (MIN) at 400 mg/day was administered. He was then transferred to our hospital on 4 September 1992 due to his deteriorated consciousness and jaundice. Exanthemata and jaundice gradually resolved with continuous administration of MIN, and he regained consciousness. The CRP gradually decreased to 9.1 mg/dl. CSF evaluation on 8 September showed marked improvement. He was discharged without neurological deficits on 22 September, 5 weeks after the onset of symptoms.

Serological results enabled us to rule out scrub typhus, typhus, Lyme disease, and leptospirosis. Immunofluorescent studies 18 days later revealed greatly elevated levels of immunoglobulin G (IgG) and IgM antibodies to Rickettsia japonica (Table 1). These findings support our diagnosis of Japanese spotted fever (JSF).


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  		TABLE 1. Titers of antibodies to R. japonica, R. typhi, R. tsutsugamushi, and Proteus strains

Case 2. A 55-year-old man injured the lower portion of his left leg on a tree prickle at a bamboo plantation on 9 April 1998. The pain lasted for several days, and the wound closed gradually. Fourteen days after the incident, he developed a low-grade fever and sore throat. The next day, his body temperature rose above 40°C. He complained of headache and nausea, which were treated unsuccessfully with antibiotics and antipyretics. Rashes covered his anterior chest wall. His symptoms worsened, and he was admitted to a local hospital on 28 April disoriented and with shaking chills. The following day, the level of his consciousness further deteriorated, and he was referred to our hospital in a state of shock.

On arrival, his body temperature was 37.5°C, and his blood pressure 96/58 mmHg while receiving a continuous infusion of dopamine. Erythematous lesions with unclear margins, measuring 5 to 10 mm in diameter, were noted on his extremities and trunk. Some of the lesions were petechial, hemorrhagic, or fused. Neither lymphadenopathy nor hepatosplenomegaly was present. His neurological condition was poor: he was delirious, and his neck was stiff. He had bilateral papilledema, although his ocular movements and pupillary reflexes to light appeared to be normal. The muscle stretch reflexes were consistently hypoactive.

Laboratory studies revealed anemia, leukocytosis, and hepatic and renal dysfunction. A CT scan of the head revealed subdural hematoma over the left fronto-temporal lobe. An electroencephalogram showed diffusely slow activity (3 to 4 Hz).

He was in septic shock causing disseminated intravascular coagulation and multiorgan failure. He required intravenous catecholamine for his shock state, artificial ventilation for respiratory failure, and hemodialysis for acute renal failure. An empirical therapy of panipenem-betamipron, fluconazole, and acyclovir was administered intravenously.

On his 2nd and 3rd hospital days, he developed generalized convulsions. He also ran an intermittent fever, and the skin eruptions were worsening. We suspected that these prolonged symptoms were caused by rickettsial diseases, and intravenous MIN was administered on 10 May. He promptly became afebrile and more responsive. On 16 May, his 19th hospital day, artificial ventilation was disconnected. He showed remarkable clinical improvement, but retained retrograde memory disturbance. Paresthesia remained in the distal portion of his legs.

An indirect immunofluorescent assay in paired sera revealed elevated titers of antibody to R. japonica, but not to Rickettsia tsutsugamushi and Rickettsia typhi. Weil-Felix tests were negative (Table 1).

Reexamination of his CSF demonstrated pleocytosis with predominant lymphocytes and elevated protein levels (Table 2). An immunofluorescent assay of paired CSF specimens showed no increase in IgM antibody to R. japonica, but a 16-fold increase in IgG antibody (Table 2). Because of the clinical symptoms and effectiveness of MIN, we reached the final diagnosis of JSF.


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  		TABLE 2. Diagnostic characteristics of CSF from patient 2

JSF is a recently recognized spotted fever group (SFG) rickettsial disease caused by R. japonica. JSF is transmitted to humans by various tick species infected with R. japonica. The triad of symptoms is fever, exanthema, and a tick bite eschar. The first known patient with JSF in Japan was reported as having SFG rickettsioses in 1984 (7). The causative agent of JSF was isolated from the serum of a patient in 1986 (14), was proven to represent a new type of SFG rickettsia (10, 15), and was named R. japonica (16).

The clinical characteristics of JSF are similar to those of scrub typhus. However, lymphadenopathy and hepatosplenomegaly are less common in JSF (6). The incubation period of JSF is about 7 days; the disease begins with an abrupt onset of a fever of 40°C or higher. Skin rashes tend to develop in the extremities and then spread to the trunk and entire body (6, 7), with some becoming petechial. The presence of exanthemata on the palms and soles is the symptom specific to JSF. The tick bite eschar in JSF patients is generally smaller than that in patients with scrub typhus. Clinically, JSF appears to be less severe than scrub typhus; however, complications may include disseminated intravascular coagulation, respiratory failure (4), or shock (3). Fatal cases have not yet been reported.

Of the five rickettsial diseases (typhus, spotted fever, scrub typhus, Q fever, and trench fever), the first three commonly cause central nervous system (CNS) infection (8). Table 3 shows the clinical features and laboratory findings of four patients with JSF with meningitis, including ours. JSF occasionally runs a severe course with CNS involvement, as described in our second case, and may be fatal. While only two patients with JSF suffered from obvious CNS involvement in the literature (2, 5), possible CNS involvement was suggested in several others, who had such symptoms as back stiffness (9) and a psychotic state with stupor (1).


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  		TABLE 3. Clinical features and laboratory findings in four cases of JSF with meningitisa

In Japan, JSF tended to be endemic to areas of warm climate that border the Pacific Ocean. JSF is most prevalent from April to October (6). In northeastern Japan, scrub typhus is prevalent in May and June, while in southwestern Japan, it is prevalent in November and December. It is interesting to note that even in the same prefectures no simultaneous peaks in the prevalence of JSF and scrub typhus have been observed (6).

Pleocytosis and elevated protein levels were measured in the second patient's CSF during the recovery stage (Table 2). Elevated IgG antibody to R. japonica was measured in paired CSF specimens, indicating an association between meningitis and R. japonica. A few published reports describe the antibody titer to rickettsiae in CSF. Rickettsial DNAs were amplified by using nested PCR for 6 of 25 CSF specimens with scrub typhus (11). This study suggests that R. tsutsugamushi can cause CNS effects and that scrub typhus should be considered a causative agent of meningitis. The CSF of patients with rickettsial infection generally reveals pleocytosis with lymphocytes predominant. Therefore, JSF should be also included in the differential diagnoses of aseptic meningitis, especially in areas of JSF endemicity.

MIN, doxycycline, and new quinolones are effective treatments for JSF, while ß-lactams and aminoglycosides are ineffective (12). An in vitro susceptibility study (13) demonstrated that MIN is the most potent known antibiotic for JSF. Early clinical diagnosis and prompt initiation of therapeutic doses of MIN are essential to prevent exacerbation of the disease.

ACKNOWLEDGMENTS

We are grateful to F. Mahara, Mahara Hospital, for his advice and to T. Katayama, Kanagawa Prefecture Public Health Laboratory, H. Fujita, Ohara Research Laboratory and T. Yumisashi, Osaka Prefecture Institute of Public Health, for assays of patient sera.


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FOOTNOTES
 
* Corresponding author. Present address: Department of Neurology, Okanami General Hospital, 1734 Kuwa-machi, Ueno-shi, Mie 518-0842, Japan. Phone: 81-595-21-3135. Fax: 81-595-21-523. E-mail: jkawa{at}nyc.odn.ne.jp. Back

FOOTNOTES

{dagger} Present address: Department of Immunology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo 187-8502, Japan. Back

REFERENCES

    1
  1. Funato, T., Y. Kitamura, A. Kawamura, and T. Uchida. 1988. Rickettsiosis of spotted fever group encountered in Muroto area of Shikoku, Japan—clinical and epidemiological features of 23 cases. J. Jpn. Assoc. Infect. Dis. 62:783-791. (In Japanese.)
    2. 2
  2. Iwamoto, K., F. Nishimura, Y. Yoshino, J. Mihara, T. Okabe, H. Kameda, and T. Kubagawa. 1988. A case of spotted fever with central nervous system involvement. J. Jpn. Assoc. Infect. Dis. 62:1192-1196. (In Japanese.)
    3. 3
  3. Iwasaki, H., F. Mahara, N. Takada, H. Fujita, and T. Ueda. 2001. Fulminant Japanese spotted fever associated with hypercytokinemia. J. Clin. Microbiol. 39:2341-2343.[Abstract/Free Full Text]
    4. 4
  4. Kodama, K., T. Noguchi, and Y. Chikahira. 2000. Japanese spotted fever complicated by acute respiratory failure. J. Jpn. Assoc. Infect. Dis. 74:162-165. (In Japanese.)
    5. 5
  5. Kodama, K., T. Senba, H. Yamauchi, Y. Chikahira, and H. Fujita. 2001. A patient with Japanese spotted fever complicated by meningoencephlitis. J. Jpn. Assoc. Infect. Dis. 75:812-814. (In Japanese.)
    6. 6
  6. Mahara, F. 1997. Japanese spotted fever. Report of 31 cases and review of the literature. Emerg. Infect. Dis. 3:105-111.[Medline]
    7. 7
  7. Mahara, F., K. Koga, S. Sawada, T. Taniguchi, T. Shigemi, and T. Suto. 1985. The first report of the rickettsial infections of spotted fever group in Japan. Three clinical cases. J. Jpn. Assoc. Infect. Dis. 59:1165-1172. (In Japanese.)
    8. 8
  8. Miller, J. R., and B. Jubelt. 1995. Bacterial infections, p. 125-127. In L. P. Rowland (ed.), Merritt's textbook of neurology, 9th ed. Williams and Wilkins, Baltimore, Md.
    9. 9
  9. Oka, N., Y. Kato, S. Dekio, T. Nishio, A. Itagaki, T. Uchiyama, and T. Uchida. 1990. A case of spotted fever group rickettsiosis first encountered in Shimane Prefecture, Japan. J. Jpn. Assoc. Infect. Dis. 64:136-142. (In Japanese.)
    10. 10
  10. Okada, T., Y. Tange, and Y. Kobayashi. 1990. Causative agent of spotted fever group rickettsiosis in Japan. Infect. Immun. 58:887-892.[Abstract/Free Full Text]
    11. 11
  11. Pai, H., S. Sohn, Y. Seong, S. Kee, W. H. Chang, and K. W. Choe. 1997. Central nervous system involvement in patients with scrub typhus. Clin. Infect. Dis. 24:436-440.[Medline]
    12. 12
  12. Suto, T. 1990. Rickettsial infections. J. Jpn. Soc. Intern. Med. 79:21-25. (In Japanese.)
    13. 13
  13. Suto, T., H. Hatakeyama, R. Ito, Y. Nakamura, and F. Mahara. 1989. In vitro susceptibility of a strain of rickettsia recently isolated from a case of Japanese spotted fever to chemotherapeutic agents. J. Jpn. Assoc. Infect. Dis. 63:35-38. (In Japanese.)
    14. 14
  14. Uchida, T., F. Tashiro, T. Funato, and K. Kitamura. 1986. Isolation of a spotted fever group rickettsia from a patient with febrile exanthematous illness in Shikoku. Jpn. Microbiol. Immunol. 30:1323-1326.
    15. 15
  15. Uchida, T., T. Uchiyama, K. Kumano, and D. H. Walker. 1992. Rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in Japan. Int. J. Syst. Bacteriol. 42:303-305.[Abstract/Free Full Text]
    16. 16
  16. Uchida, T., X. Yu, T. Uchiyama, and D. H. Walker. 1989. Identification of a unique spotted fever group rickettsia from humans in Japan. J. Infect. Dis. 159:1122-1126.[Medline]

Journal of Clinical Microbiology, October 2002, p. 3874-3876, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3874-3876.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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