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Journal of Clinical Microbiology, November 2002, p. 4289-4294, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4289-4294.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Dissemination of New Methicillin-Resistant Staphylococcus aureus Clones in the Community

Keiko Okuma,1 Kozue Iwakawa,1 John D. Turnidge,2 Warren B. Grubb,3 Jan M. Bell,2 Frances G. O'Brien,3 Geoffrey W. Coombs,4 John W. Pearman,4 Fred C. Tenover,5 Maria Kapi,1 Chuntima Tiensasitorn,1 Teruyo Ito,1 and Keiichi Hiramatsu1*

Department of Bacteriology, Juntendo University, Tokyo, Japan,1 Department of Microbiology and Infectious Diseases, Women's and Children's Hospital, North Adelaide,2 Molecular Genetics Research Unit, School of Biomedical Sciences, Curtin University of Technology,3 Department of Microbiology and Infectious Diseases, Royal Perth Hospital, Perth, Australia,4 National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303335

Received 25 April 2002/ Returned for modification 21 June 2002/ Accepted 6 August 2002


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ABSTRACT
 
Multiple methicillin-resistant Staphylococcus aureus (MRSA) clones carrying type IV staphylococcal cassette chromosome mec were identified in the community-acquired MRSA strains of both the United States and Australia. They multiplied much faster than health-care-associated MRSA and were resistant to fewer non-beta-lactam antibiotics. They seem to have been derived from more diverse S. aureus populations than health-care-associated MRSA strains.


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TEXT
 
Methicillin-resistant Staphylococcus aureus (MRSA), besides having established itself as a major hospital pathogen, is now beginning to prevail in the wider community as well (1, 3-5). However, we do not know if the subgroup of MRSA designated community-acquired MRSA (C-MRSA) share a common origin of derivation with the other subgroup of MRSA in hospitals, namely the health-care-associated MRSA (H-MRSA). The majority of H-MRSA strains carry one of the three types of staphylococcal cassette chromosome mec (SCCmec) as the methicillin resistance determinant on their chromosomes (19, 22). However, members of our group have recently identified a novel SCCmec, designated type IV, in the C-MRSA strains isolated at a Chicago children's hospital (23). This raised a possibility that C-MRSA might have an origin of derivation distinct from that of H-MRSA, and type-IV SCCmec could be its unique genetic marker (14). To further test this view, we now analyzed 23 well-characterized C-MRSA strains (2-4, 24-26, 28) whose sources of isolation were not associated with risk factors for H-MRSA infection (e.g., recent hospitalization, recent surgery, residence in a long-term care facility, drug use, etc.) (7, 11) and 12 Australian MRSA strains designated non-multiresistant oxacillin-resistant S. aureus (NORSA) (9) and compared them with the representative H-MRSA strains. NORSA strains, though frequently isolated in hospitals, are considered to be the descendants of C-MRSA strains in Australia (10).

Table 1 shows that the majority of C-MRSA strains were susceptible to most of the non-beta-lactam antibiotics, as NORSA strains are by definition (9). Although the non-multiresistant nature of C-MRSA has been well recognized as a characteristic of C-MRSA (16), this was not without exception: strain 01083 was resistant to four non-beta-lactam antibiotics (Table 1). This indicates that though it is a rare occurrence, C-MRSA strains may also acquire resistance to non-beta-lactam antibiotics, presumably through exposure to the antibiotics.


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  		TABLE 1. Genotyping and antibiogram of tested MRSA strains

Table 1 also shows that C-MRSA/NORSA strains had relatively lower levels of oxacillin and imipenem resistance than H-MRSA strains (with the exceptions of N315 and 85/2082) (20). This indicated that they had the heterogeneous methicillin resistance phenotype, which was confirmed by population analysis (Fig. 1). MW2, a representative C-MRSA strain (2), possessed typical heterogeneous subpopulations of resistant cells, whereas the "truly" (i.e., mecA-negative) methicillin-susceptible strain 476, the putative progenitor strain of MW2 (see below), did not have the resistant subpopulations. Mu3, a typical H-MRSA strain, on the other hand, had a distinct pattern of resistance called homogeneous methicillin resistance. This implied that unlike H-MRSA strains, C-MRSA strains were not selected out by the exposure to these potent beta-lactam antibiotics used in the hospital, testifying further to their predominant propagation occurring in the community.



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  		FIG. 1. C-MRSA strain shows heterogeneous phenotypic expression of methicillin resistance. Analysis of resistant subpopulations of the C-MRSA strain MW2, the related MSSA strain 476, and strain Mu3, with heterogeneous resistance to vancomycin, was performed with oxacillin (A), ceftriaxone (B), and imipenem (C) as described previously (13). Ceftriaxone was the antibiotic used in vain to treat the patient infected with MW2 (3). MW2 is an American Midwest MRSA strain representing the major C-MRSA (see the text). Strain 476 is an MSSA strain sharing its MLST allotype with MW2 (see Table 1). Mu3 is a representative H-MRSA strain with heterogeneous resistance to vancomycin (13). It is noted that MW2 contains subpopulations resistant to each of the three beta-lactam antibiotics.

C-MRSA/NORSA strains grew significantly faster than H-MRSA strains: the mean doubling times (8) of the former group of strains were 28.79 ± 7.09 and 28.24 ± 2.48 min, respectively, whereas that for the latter was 38.81 ± 7.01 min (see Table 1). The difference was statistically significant (P value of <0.0001 by t test). This high growth rate may be a prerequisite in the absence of antibiotics for C-MRSA to achieve successful colonization of humans by outcompeting the numerous bacterial species in the environment.

The MRSA genotype is the sum of the SCCmec type and the type of its recipient chromosome (12). First, by using multiple locus sequence typing (MLST), we identified the chromosome genotype of the test strains. Enright et al. reported that 356 of 359 MRSA strains from 20 countries were classified into only five clonal complexes (CCs), CC5, CC8, CC22, CC30, and CC45, with the rest, three strains, possessing sequence types (STs) of rare occurrence (6). All the 11 H-MRSA strains used in this study were reasonably classified into three of these five CCs (Table 1). However, 35 C-MRSA/NORSA strains possessed 10 different STs that constituted one singleton (ST75) and seven CCs that, surprisingly, included all five H-MRSA CCs described above (Table 1).

Among the seven C-MRSA CCs, especially notable was CC1, which contained the internationally dominant C-MRSA strains; eight U.S. strains represented by MW2 and six Australian strains belonged to this clonal complex. Remarkably, no H-MRSA strains belonged to this complex (6). Curiously, a highly virulent methicillin-susceptible S. aureus (MSSA) strain, 476, whose whole genome sequence has been determined, belongs to this complex (http://www.mlst.net/new/index.htm). MSSA 476 and two NORSA strains belonging to CC1 even shared an identical pulsed-field gel electrophoresis (PFGE) pattern (Table 1). Detailed comparison revealed that the only significant difference between the two chromosomes was the presence of type IV SCCmec in MW2, which indicated that strain 476 represented the progenitor MSSA strain from which MW2 was generated by acquiring type IV SCCmec.

The pattern of clonal distribution of the 35 C-MRSA/NORSA strains was statistically distinct from that of 359 MRSA strains analyzed in a previous study plus 11 H-MRSA strains used in this study (P value of <0.000001 by Fisher's exact test). This clearly indicated that distinct clonal populations were successfully propagated as C-MRSA/NORSA and H-MRSA, presumably through different selective pressures exerted on them, e.g., fast-growing S. aureus or S. epidermidis strains for the former and exposure to multiple antibiotics for the latter.

The MLST data, despite the small number of tested strains, indicated that C-MRSA/NORSA strains were generated from S. aureus clones of much more diverse genetic backgrounds than expected. This was also supported by PFGE analysis (Table 1), which showed that the C-MRSA/NORSA strains were classified into nine unrelated groups according to the criteria described by Tenover et al. (27). Moreover, these strains consisted of producers of as many as seven coagulase isotypes (Table 1). Since only eight coagulase isotypes are known among S. aureus strains isolated from various sources (18), this also supported the view that C-MRSA/NORSA represents diverse S. aureus genomes as the origin of derivation.

Next, we determined SCCmec types by PCR typing of the mec gene complex and ccr gene complex as described previously (19, 21). Table 2 and Fig. 2 show the nucleotide sequences and locations of the primers used (15, 19, 21). In contrast to the heterogeneity of C-MRSA/NORSA chromosomes demonstrated above, all except for three strains harbored type IV SCCmec, and the remaining three harbored a novel SCCmec carrying the class C2 mec gene complex (21) (Fig. 2). None of the C-MRSA/NORSA strains possessed any of the three types of SCCmec which the majority of epidemic H-MRSA strains possess (19).


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  		TABLE 2. Primer sets used for identifying SCCmec



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  		FIG. 2. Illustrative representation of various types of SCCmec. SCCmec type is defined by the combination of the type of ccr gene complex and the class of mec gene complex. Type I SCCmec is defined by the combination of type 1 ccr and class B mec (IS1272-{Delta}mecR1-mecA); type II is defined by type 2 ccr and class A mec (mecI-mecR1-mecA); type III is defined by type 3 ccr and class A mec; and type IV is defined by type 2 ccr and class B mec. Type IV SCCmec is further classified into subtypes (type IVa and type IVb) based on the sequence difference in the J1 region of SCCmec (J stands for "junkyard"). Positions of primers used in this study to identify and type SCCmec are shown (see Table 2 for the nucleotide sequence of each primer). The allelic class of mec gene complex is determined by PCR detection of the presence or absence of IS1272, mecI, and mecR1 in two domains (PB, penicillin-binding domain; and MS, membrane-spanning domain), mecA, and IS431mec. PCR amplification was performed using 2.5 U of Ex Taq (Takara Shuzo Co., Ltd., Kyoto, Japan) in 50 µl of reaction mixture. Thermal cycling was set at 30 cycles (30 s for denaturation at 94°C, 1 min for annealing at 50°C, and 2 min for elongation at 72°C) using the Gene Amp PCR system 9600 (Perkin-Elmer, Wellesley, Mass.). For the detection of mecA-IS431mec, a long-range PCR method was used, set at 10 cycles (15 s for denaturation at 94°C, 30 s for annealing at 50°C, and 8 min for elongation at 68°C) followed by 20 cycles (15 s for denaturation at 94°C, 30 s for annealing at 50°C, and 12 min for elongation at 68°C). Note that this study identified a new type of SCCmec for three C-MRSA strains that carried the class C2 mec gene complex (21). The sequencing of the entire SCCmec is now ongoing.

It is not clear at this moment why type IV SCCmec is prevalent in C-MRSA/NORSA strains. However, type IV SCCmec is short (21 to 25 kb) compared to the three SCCmecs prevalent in H-MRSA strains (34 to 67 kb) and lacks any antibiotic resistance genes other than mecA (23) (Fig. 2). This evidently corresponds to the non-multiresistant nature of C-MRSA/NORSA and may alleviate the fitness cost paid by H-MRSA strains carrying big SCCmecs with multiple-drug resistance determinants.

Although we need to explore further the reason why type IV SCCmec is prevalent in C-MRSA strains, it seems clear that we are witnessing the emergence and expansion of new MRSA clones in the community. These clones are different from any of the major H-MRSA clones in the world that we have identified by using SCCmec typing and ribotyping combinations (12, 17). In this study we realized that the antibiogram is not completely reliable in discriminating C-MRSA from H-MRSA, nor is the phenotypic expression of methicillin resistance. Even epidemiological information is not sufficient, since, for example, many C-MRSA strains have been carried in Australian hospitals (29). Therefore, no reliable judgment can be made as to whether the strain isolated in the hospital is H-MRSA or C-MRSA even based on the timing of isolation of the strains after admission to hospital. In this regard, SCCmec and MLST typing will become more important in the years to come for discrimina-tion of numerous C-MRSA strains prevailing in both com-munity and hospitals by reference to their ancestral MRSA clones (12).


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ACKNOWLEDGMENTS
 
We thank T. Naimi for providing us C-MRSA strains and for fruitful discussion. We also thank M. C. Enright for kind instruction on MLST and the BURST program and Susan Johnson and David Boxrud in Minnesota and Suwanna Trakulsomboon, Mantana Jamklang, and Fumihiko Takeuchi in Japan for their excellent technical assistance. We also thank Yuh Morimoto for her help in preparing the manuscript.

This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (13226114) from The Ministry of Education, Science, Sports, Culture and Technology of Japan and the Core University System Exchange Programme under the Japan Society for the Promotion of Science, coordinated by the University of Tokyo Graduate School of Medicine and Mahidol University. The work was also supported by a Grant for International Health Cooperation Research (11C-4) from the Ministry of Health and Welfare, by the Research for the Future Program of the Japan Society for the Promotion of Science, and by the National Health and Medical Research Foundation of Australia and the Health Department of Western Australia.


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FOOTNOTES
 
* Corresponding author. Mailing address: Department of Bacteriology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-Ku, Tokyo, Japan, 113-8421. Phone: 81 (3) 5802-10410. Fax: 81 (3) 5684-7830. E-mail: hiram{at}med.juntendo.ac.jp. Back


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REFERENCES
 
    1
  1. Alghaithy, A. A., N. E. Bilal, M. Gedebou, and A. H. Weily. 2000. Nasal carriage and antibiotic resistance of Staphylococcus aureus isolates from hospital and non-hospital personnel in Abha, Saudi Arabia. Trans. R. Soc. Trop. Med. Hyg. 94:504-507.[CrossRef][Medline]
    2. 2
  2. Baba, T., F. Takeuchi, M. Kuroda, H. Yuzawa, K. Aoki, A. Oguchi, Y. Nagai, N. Iwama, K. Asano, T. Naimi, H. Kuroda, L. Cui, K. Yamamoto, and K. Hiramatsu. 2002. Genome and virulence determinants of high virulence community-acquired MRSA. Lancet 359:1819-1827.[CrossRef][Medline]
    3. 3
  3. Centers for Disease Control and Prevention. 1999. Four pediatric deaths from community-acquired methicillin-resistant Staphylococcus aureus—Minnesota and North Dakota, 1997-1999. Morb. Mortal. Wkly. Rep. 48:707-710.
    4. 4
  4. Centers for Disease Control and Prevention. 2001. Methicillin-resistant Staphylococcus aureus skin or soft tissue infections in a state prison—Mississippi, 2000. Morb. Mortal. Wkly. Rep. 50:919-922.[Medline]
    5. 5
  5. Chambers, H. 2001. The changing epidemiology of Staphylococcus aureus? Emerg. Infect. Dis. 7:178-182.[Medline]
    6. 6
  6. Enright, M. C., D. Robinson, G. Randle, E. J. Feil, H. Grundmann, and B. G. Spratt. 2002. The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proc. Natl. Acad. Sci. USA 99:7687-7692.[Abstract/Free Full Text]
    7. 7
  7. Garner, J. S., W. R. Jarvis, T. G. Emori, T. C. Horan, and J. M. Hughes. 1988. CDC definitions for nosocomial infections, 1998. Am. J. Infect. Control 16:128-140.[CrossRef][Medline]
    8. 8
  8. Gerhardt, P., R. G. E. Murray, W. A. Wood, and N. R. Krieg. 1994. Methods for general and molecular bacteriology, p. 270-271. American Society for Microbiology, Washington, D.C.
    9. 9
  9. Gosbell, I. B., J. Mercer, S. A. Neville, K. G. Chant, and R. Munro. 2001. Community-acquired, non-multiresistant oxacillin-resistant Staphylococcus aureus (NORSA) in South Western Sydney. Pathology 33:206-210.[Medline]
    10. 10
  10. Gosbell, I. B., J. Mercer, S. A. Neville, S. A. Crone, K. G. Chant, B. B. Jalaludin, and R. Munro. 2001. Non-multiresistant and multiresistant methicillin-resistant Staphylococcus aureus in community-acquired infections. Med. J. Aust. 174:627-630.[Medline]
    11. 11
  11. Herold, B. C., L. C. Immergluck, M. C. Maranan, D. S. Lauderdale, R. E. Gaskin, S. Boyle-Vavra, C. D. Leitch, and R. S. Daum. 1998. Community-acquired methicillin-resistant Staphylococcus aureus in children with no identified predisposing risk. JAMA 279:593-598.[Abstract/Free Full Text]
    12. 12
  12. Hiramatsu, K. 1995. Molecular evolution of MRSA. Microbiol. Immunol. 39:531-543.[Medline]
    13. 13
  13. Hiramatsu, K., N. Aritaka, H. Hanaki, S. Kawasaki, Y. Hosoda, S. Hori, Y. Fukuchi, and I. Kobayashi. 1997. Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin. Lancet 350:1670-1673.[CrossRef][Medline]
    14. 14
  14. Hiramatsu, K., L. Cui, M. Kuroda, and T. Ito. 2001. The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trends Microbiol. 9:486-493.[CrossRef][Medline]
    15. 15
  15. Hiramatsu, K., H. Kihara, and T. Yokota. 1992. Analysis of borderline-resistant strains of methicillin-resistant Staphylococcus aureus using polymerase chain reaction. Microbiol. Immunol. 36:445-453.[Medline]
    16. 16
  16. Hiramatsu, K., K. Okuma, X. X. Ma, M. Yamamoto, S. Hori, and M. Kapi. 2002. New trends in Staphylococcus aureus infections: glycopeptide resistance in hospital and methicillin resistance in the community. Curr. Opin. Infect. Dis. 15:407-413.[Medline]
    17. 17
  17. Hiramatsu, K., T. Ito, and H. Hanaki. 1999. Mechanisms of methicillin and vancomycin resistance in Staphylococcus aureus, p. 221-242. In R. G. Finch and R. J. Williams (ed.), Bailliere's clinical infectious diseases, vol. 5. Bailliere Tindall, London, United Kingdom.
    18. 18
  18. Igarashi, H. 1993. Producibility of staphylococcal enterotoxins and toxic shock syndrome toxin-1 on methicillin-resistant Staphylococcus aureus. Igakunoayumi 166:274-278. (In Japanese.)
    19. 19
  19. Ito, T., Y. Katayama, K. Asada, N. Mori, K. Tsutsumimoto, C. Tiesasitorn, and K. Hiramatsu. 2001. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 45:1323-1336.[Abstract/Free Full Text]
    20. 20
  20. Johnson, A. P., H. M. Aucken, S. Cavendish, M. Ganner, M. C. Wale, M. Warner, D. M. Livermore, B. D. Cookson, and the UK EARSS Participants. 2001. Dominance of EMRSA-15 and -16 among MRSA causing nosocomial bacteraemia in the UK: analysis of isolates from the European Antimicrobial Resistance Surveillance System (EARSS). J. Antimicrob. Chemother. 48:143-144.[Free Full Text]
    21. 21
  21. Katayama, Y., T. Ito, and K. Hiramatsu. 2001. Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: role of IS431-mediated mecI deletion in expression of resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus. Antimicrob. Agents Chemother. 45:1955-1963.[Abstract/Free Full Text]
    22. 22
  22. Katayama, Y., T. Ito, and K. Hiramatsu. 2000. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 44:1549-1555.[Abstract/Free Full Text]
    23. 23
  23. Ma, X. X., T. Ito, C. Tiensasitorn, M. Jamklang, P. Chongtrakool, S. Boyle-Vavra, R. S. Daum, and K. Hiramatsu. 2002. Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob. Agents Chemother. 46:1147-1152.[Abstract/Free Full Text]
    24. 24
  24. Naimi, T. S., K. H. LeDell, D. J. Boxrud, A. V. Groom, C. D. Steward, S. K. Johnson, J. M. Besser, C. O'Boyle, R. N. Danila, J. E. Cheek, M. T. Osterholm, K. A. Moore, and K. E. Smith. 2001. Epidemiology and clonality of community-acquired methicillin-resistant Staphylococcus aureus in Minnesota, 1996-1998. Clin. Infect. Dis. 33:990-996.[CrossRef][Medline]
    25. 25
  25. Nimmo, G. R., J. Schooneveldt, G. O'Kane, B. McCall, and A. Vickery. 2000. Community acquisition of gentamicin-sensitive methicillin-resistant Staphylococcus aureus in southeast Queensland, Australia. J. Clin. Microbiol. 38:3926-3931.[Abstract/Free Full Text]
    26. 26
  26. O'Brien, F. G., J. Pearman, M. Gracey, T. V. Riley, and W. B. Grubb. 1999. Community strain of methicillin-resistant Staphylococcus aureus involved in a hospital outbreak. J. Clin. Microbiol. 37:2858-2862.[Abstract/Free Full Text]
    27. 27
  27. Tenover, F. C., R. D. Arbeit, R. V. Goering, P. A. Mickelsen, P. A. Mickelsen, B. E. Murry, D. H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33:2233-2239.[Medline]
    28. 28
  28. Timothy, F. J., E. K. Molly, S. S. Porter, B. Michael, and S. William. 2002. An outbreak of community-acquired foodborne illness caused by methicillin-resistant Staphylococcus aureus. Emerg. Infect. Dis. 8:82-84.[Medline]
    29. 29
  29. Turnidge, J. D., and J. Bell. 2000. Methicillin-resistant Staphylococcal aureus evolution in Australia over 35 years. Microb. Drug Resist. 6:223-229.[Medline]

Journal of Clinical Microbiology, November 2002, p. 4289-4294, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4289-4294.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Cheung, T. K. M., Chu, Y. W., Chu, M. Y., Tsang, V. Y. M., Lo, J. Y. C. (2008). Panton-Valentine leukocidin-positive meticillin-resistant Staphylococcus aureus in the community in Hong Kong. J Med Microbiol 57: 1440-1443 [Full Text]  
  • Ma, X. X., Ito, T., Kondo, Y., Cho, M., Yoshizawa, Y., Kaneko, J., Katai, A., Higashiide, M., Li, S., Hiramatsu, K. (2008). Two Different Panton-Valentine Leukocidin Phage Lineages Predominate in Japan. J. Clin. Microbiol. 46: 3246-3258 [Abstract] [Full Text]  
  • Berglund, C., Ito, T., Ikeda, M., Ma, X. X., Soderquist, B., Hiramatsu, K. (2008). Novel Type of Staphylococcal Cassette Chromosome mec in a Methicillin-Resistant Staphylococcus aureus Strain Isolated in Sweden. Antimicrob. Agents Chemother. 52: 3512-3516 [Abstract] [Full Text]  
  • Nakaminami, H., Noguchi, N., Ikeda, M., Hasui, M., Sato, M., Yamamoto, S., Yoshida, T., Asano, T., Senoue, M., Sasatsu, M. (2008). Molecular epidemiology and antimicrobial susceptibilities of 273 exfoliative toxin-encoding-gene-positive Staphylococcus aureus isolates from patients with impetigo in Japan. J Med Microbiol 57: 1251-1258 [Abstract] [Full Text]  
  • Lalani, T., Federspiel, J. J., Boucher, H. W., Rude, T. H., Bae, I.-G., Rybak, M. J., Tonthat, G. T., Corey, G. R., Stryjewski, M. E., Sakoulas, G., Chu, V. H., Alder, J., Steenbergen, J. N., Luperchio, S. A., Campion, M., Woods, C. W., Fowler, V. G. (2008). Associations between the Genotypes of Staphylococcus aureus Bloodstream Isolates and Clinical Characteristics and Outcomes of Bacteremic Patients. J. Clin. Microbiol. 46: 2890-2896 [Abstract] [Full Text]  
  • Goering, R. V., Shawar, R. M., Scangarella, N. E., O'Hara, F. P., Amrine-Madsen, H., West, J. M., Dalessandro, M., Becker, J. A., Walsh, S. L., Miller, L. A., van Horn, S. F., Thomas, E. S., Twynholm, M. E. (2008). Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Global Clinical Trials. J. Clin. Microbiol. 46: 2842-2847 [Abstract] [Full Text]  
  • Tenover, F. C., McAllister, S., Fosheim, G., McDougal, L. K., Carey, R. B., Limbago, B., Lonsway, D., Patel, J. B., Kuehnert, M. J., Gorwitz, R. (2008). Characterization of Staphylococcus aureus Isolates from Nasal Cultures Collected from Individuals in the United States in 2001 to 2004. J. Clin. Microbiol. 46: 2837-2841 [Abstract] [Full Text]  
  • Chua, T., Moore, C. L., Perri, M. B., Donabedian, S. M., Masch, W., Vager, D., Davis, S. L., Lulek, K., Zimnicki, B., Zervos, M. J. (2008). Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus Bloodstream Isolates in Urban Detroit. J. Clin. Microbiol. 46: 2345-2352 [Abstract] [Full Text]  
  • Lu, P.-L., Tsai, J.-C., Chiu, Y.-W., Chang, F.-Y., Chen, Y.-W., Hsiao, C.-F., Siu, L. K. (2008). Methicillin-resistant Staphylococcus aureus carriage, infection and transmission in dialysis patients, healthcare workers and their family members. Nephrol Dial Transplant 23: 1659-1665 [Abstract] [Full Text]  
  • Noto, M. J., Fox, P. M., Archer, G. L. (2008). Spontaneous Deletion of the Methicillin Resistance Determinant, mecA, Partially Compensates for the Fitness Cost Associated with High-Level Vancomycin Resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 52: 1221-1229 [Abstract] [Full Text]  
  • Chung, H.-J., Jeon, H.-S., Sung, H., Kim, M.-N., Hong, S.-J. (2008). Epidemiological Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates from Children with Eczematous Atopic Dermatitis Lesions. J. Clin. Microbiol. 46: 991-995 [Abstract] [Full Text]  
  • Noto, M. J., Kreiswirth, B. N., Monk, A. B., Archer, G. L. (2008). Gene Acquisition at the Insertion Site for SCCmec, the Genomic Island Conferring Methicillin Resistance in Staphylococcus aureus. J. Bacteriol. 190: 1276-1283 [Abstract] [Full Text]  
  • Herman, R. A., Kee, V. R., Moores, K. G., Ross, M. B. (2008). Etiology and treatment of community-associated methicillin-resistant Staphylococcus aureus. Am J Health Syst Pharm 65: 219-225 [Abstract] [Full Text]  
  • Campbell, S. J., Deshmukh, H. S., Nelson, C. L., Bae, I.-G., Stryjewski, M. E., Federspiel, J. J., Tonthat, G. T., Rude, T. H., Barriere, S. L., Corey, R., Fowler, V. G. Jr. (2008). Genotypic Characteristics of Staphylococcus aureus Isolates from a Multinational Trial of Complicated Skin and Skin Structure Infections. J. Clin. Microbiol. 46: 678-684 [Abstract] [Full Text]  
  • Larsen, A. R., Bocher, S., Stegger, M., Goering, R., Pallesen, L. V., Skov, R. (2008). Epidemiology of European Community-Associated Methicillin-Resistant Staphylococcus aureus Clonal Complex 80 Type IV Strains Isolated in Denmark from 1993 to 2004. J. Clin. Microbiol. 46: 62-68 [Abstract] [Full Text]  
  • Miragaia, M., Carrico, J. A., Thomas, J. C., Couto, I., Enright, M. C., de Lencastre, H. (2008). Comparison of Molecular Typing Methods for Characterization of Staphylococcus epidermidis: Proposal for Clone Definition. J. Clin. Microbiol. 46: 118-129 [Abstract] [Full Text]  
  • Faria, N. A., Carrico, J. A., Oliveira, D. C., Ramirez, M., de Lencastre, H. (2008). Analysis of Typing Methods for Epidemiological Surveillance of both Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Strains. J. Clin. Microbiol. 46: 136-144 [Abstract] [Full Text]  
  • Watanabe, S., Ito, T., Hiramatsu, K. (2007). Susceptibilities of healthcare- and community-associated methicillin-resistant staphylococci to the novel des-F(6)-quinolone DX-619. J Antimicrob Chemother 60: 1384-1387 [Abstract] [Full Text]  
  • Witte, W., Strommenger, B., Cuny, C., Heuck, D., Nuebel, U. (2007). Methicillin-resistant Staphylococcus aureus containing the Panton-Valentine leucocidin gene in Germany in 2005 and 2006. J Antimicrob Chemother 60: 1258-1263 [Abstract] [Full Text]  
  • Park, C., Lee, D.-G., Kim, S. W., Choi, S.-M., Park, S. H., Chun, H.-S., Choi, J.-H., Yoo, J.-H., Shin, W. S., Kang, J. H., Kim, J. H., Lee, S. Y., Kim, S. M., Pyun, B. Y. (2007). Predominance of Community-Associated Methicillin-Resistant Staphylococcus aureus Strains Carrying Staphylococcal Chromosome Cassette mec Type IVA in South Korea. J. Clin. Microbiol. 45: 4021-4026 [Abstract] [Full Text]  
  • Milheirico, C., Oliveira, D. C., de Lencastre, H. (2007). Update to the Multiplex PCR Strategy for Assignment of mec Element Types in Staphylococcus aureus. Antimicrob. Agents Chemother. 51: 3374-3377 [Abstract] [Full Text]  
  • Amorim, M. L., Faria, N. A., Oliveira, D. C., Vasconcelos, C., Cabeda, J. C., Mendes, A. C., Calado, E., Castro, A. P., Ramos, M. H., Amorim, J. M., de Lencastre, H. (2007). Changes in the Clonal Nature and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Isolates Associated with Spread of the EMRSA-15 Clone in a Tertiary Care Portuguese Hospital. J. Clin. Microbiol. 45: 2881-2888 [Abstract] [Full Text]  
  • Stephens, A. J., Huygens, F., Giffard, P. M. (2007). Systematic Derivation of Marker Sets for Staphylococcal Cassette Chromosome mec Typing. Antimicrob. Agents Chemother. 51: 2954-2964 [Abstract] [Full Text]  
  • Brady, J. M., Stemper, M. E., Weigel, A., Chyou, P.-H., Reed, K. D., Shukla, S. K. (2007). Sporadic "Transitional" Community-Associated Methicillin-Resistant Staphylococcus aureus Strains from Health Care Facilities in the United States. J. Clin. Microbiol. 45: 2654-2661 [Abstract] [Full Text]  
  • Rossney, A. S., Shore, A. C., Morgan, P. M., Fitzgibbon, M. M., O'Connell, B., Coleman, D. C. (2007). The Emergence and Importation of Diverse Genotypes of Methicillin-Resistant Staphylococcus aureus (MRSA) Harboring the Panton-Valentine Leukocidin Gene (pvl) Reveal that pvl Is a Poor Marker for Community-Acquired MRSA Strains in Ireland. J. Clin. Microbiol. 45: 2554-2563 [Abstract] [Full Text]  
  • Milheirico, C., Oliveira, D. C., de Lencastre, H. (2007). Multiplex PCR strategy for subtyping the staphylococcal cassette chromosome mec type IV in methicillin-resistant Staphylococcus aureus: 'SCCmec IV multiplex'. J Antimicrob Chemother 60: 42-48 [Abstract] [Full Text]  
  • Goldstein, F., Perutka, J., Cuirolo, A., Plata, K., Faccone, D., Morris, J., Sournia, A., Kitzis, M. D., Ly, A., Archer, G., Rosato, A. E. (2007). Identification and Phenotypic Characterization of a {beta}-Lactam-Dependent, Methicillin-Resistant Staphylococcus aureus Strain. Antimicrob. Agents Chemother. 51: 2514-2522 [Abstract] [Full Text]  
  • Bhattacharya, D., Carleton, H., Tsai, C. J., Baron, E. J., Perdreau-Remington, F. (2007). Differences in Clinical and Molecular Characteristics of Skin and Soft Tissue Methicillin-Resistant Staphylococcus aureus Isolates between Two Hospitals in Northern California. J. Clin. Microbiol. 45: 1798-1803 [Abstract] [Full Text]  
  • Goering, R. V., McDougal, L. K., Fosheim, G. E., Bonnstetter, K. K., Wolter, D. J., Tenover, F. C. (2007). Epidemiologic Distribution of the Arginine Catabolic Mobile Element among Selected Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates. J. Clin. Microbiol. 45: 1981-1984 [Abstract] [Full Text]  
  • Cookson, B. D., Robinson, D. A., Monk, A. B., Murchan, S., Deplano, A., de Ryck, R., Struelens, M. J., Scheel, C., Fussing, V., Salmenlinna, S., Vuopio-Varkila, J., Cuny, C., Witte, W., Tassios, P. T., Legakis, N. J., van Leeuwen, W., van Belkum, A., Vindel, A., Garaizar, J., Haeggman, S., Olsson-Liljequist, B., Ransjo, U., Muller-Premru, M., Hryniewicz, W., Rossney, A., O'Connell, B., Short, B. D., Thomas, J., O'Hanlon, S., Enright, M. C. (2007). Evaluation of Molecular Typing Methods in Characterizing a European Collection of Epidemic Methicillin-Resistant Staphylococcus aureus Strains: the HARMONY Collection. J. Clin. Microbiol. 45: 1830-1837 [Abstract] [Full Text]  
  • Tang, Y.-W., Kilic, A., Yang, Q., McAllister, S. K., Li, H., Miller, R. S., McCormac, M., Tracy, K. D., Stratton, C. W., Han, J., Limbago, B. (2007). StaphPlex System for Rapid and Simultaneous Identification of Antibiotic Resistance Determinants and Panton-Valentine Leukocidin Detection of Staphylococci from Positive Blood Cultures. J. Clin. Microbiol. 45: 1867-1873 [Abstract] [Full Text]  
  • Elizur, A., Orscheln, R. C., Ferkol, T. W., Atkinson, J. J., Dunne, W. M. Jr, Buller, R. S., Armstrong, J. R., Mardis, E. R., Storch, G. A., Cannon, C. L. (2007). Panton-Valentine Leukocidin-Positive Methicillin-Resistant Staphylococcus aureus Lung Infection in Patients With Cystic Fibrosis. Chest 131: 1718-1725 [Abstract] [Full Text]  
  • Hota, B., Ellenbogen, C., Hayden, M. K., Aroutcheva, A., Rice, T. W., Weinstein, R. A. (2007). Community-Associated Methicillin-Resistant Staphylococcus aureus Skin and Soft Tissue Infections at a Public Hospital: Do Public Housing and Incarceration Amplify Transmission?. Arch Intern Med 167: 1026-1033 [Abstract] [Full Text]  
  • Hanssen, A.-M., Sollid, J. U. E. (2007). Multiple Staphylococcal Cassette Chromosomes and Allelic Variants of Cassette Chromosome Recombinases in Staphylococcus aureus and Coagulase-Negative Staphylococci from Norway. Antimicrob. Agents Chemother. 51: 1671-1677 [Abstract] [Full Text]  
  • Sasaki, T., Kikuchi, K., Tanaka, Y., Takahashi, N., Kamata, S., Hiramatsu, K. (2007). Methicillin-Resistant Staphylococcus pseudintermedius in a Veterinary Teaching Hospital. J. Clin. Microbiol. 45: 1118-1125 [Abstract] [Full Text]  
  • Lee, S. M., Ender, M., Adhikari, R., Smith, J. M. B., Berger-Bachi, B., Cook, G. M. (2007). Fitness Cost of Staphylococcal Cassette Chromosome mec in Methicillin-Resistant Staphylococcus aureus by Way of Continuous Culture. Antimicrob. Agents Chemother. 51: 1497-1499 [Abstract] [Full Text]  
  • Miragaia, M., Thomas, J. C., Couto, I., Enright, M. C., de Lencastre, H. (2007). Inferring a Population Structure for Staphylococcus epidermidis from Multilocus Sequence Typing Data. J. Bacteriol. 189: 2540-2552 [Abstract] [Full Text]  
  • Hallin, M., Denis, O., Deplano, A., De Mendonca, R., De Ryck, R., Rottiers, S., Struelens, M. J. (2007). Genetic relatedness between methicillin-susceptible and methicillin-resistant Staphylococcus aureus: results of a national survey. J Antimicrob Chemother 59: 465-472 [Abstract] [Full Text]  
  • Moroney, S. M., Heller, L. C., Arbuckle, J., Talavera, M., Widen, R. H. (2007). Staphylococcal Cassette Chromosome mec and Panton-Valentine Leukocidin Characterization of Methicillin-Resistant Staphylococcus aureus Clones. J. Clin. Microbiol. 45: 1019-1021 [Abstract] [Full Text]  
  • Ghebremedhin, B., Konig, W., Witte, W., Hardy, K. J., Hawkey, P. M., Konig, B. (2007). Subtyping of ST22-MRSA-IV (Barnim epidemic MRSA strain) at a university clinic in Germany from 2002 to 2005. J Med Microbiol 56: 365-375 [Abstract] [Full Text]  
  • Hsu, L.-Y., Loomba-Chlebicka, N., Koh, Y.-L., Tan, T.-Y., Krishnan, P., Lin, R. T.-P., Tee, N. W.-S., Fisher, D. A., Koh, T.-H. (2007). Evolving EMRSA-15 epidemic in Singapore hospitals. J Med Microbiol 56: 376-379 [Abstract] [Full Text]  
  • Kondo, Y., Ito, T., Ma, X. X., Watanabe, S., Kreiswirth, B. N., Etienne, J., Hiramatsu, K. (2007). Combination of Multiplex PCRs for Staphylococcal Cassette Chromosome mec Type Assignment: Rapid Identification System for mec, ccr, and Major Differences in Junkyard Regions. Antimicrob. Agents Chemother. 51: 264-274 [Abstract] [Full Text]  
  • Weigel, L. M., Donlan, R. M., Shin, D. H., Jensen, B., Clark, N. C., McDougal, L. K., Zhu, W., Musser, K. A., Thompson, J., Kohlerschmidt, D., Dumas, N., Limberger, R. J., Patel, J. B. (2007). High-Level Vancomycin-Resistant Staphylococcus aureus Isolates Associated with a Polymicrobial Biofilm. Antimicrob. Agents Chemother. 51: 231-238 [Abstract] [Full Text]  
  • Ma, X. X., Ito, T., Chongtrakool, P., Hiramatsu, K. (2006). Predominance of Clones Carrying Panton-Valentine Leukocidin Genes among Methicillin-Resistant Staphylococcus aureus Strains Isolated in Japanese Hospitals from 1979 to 1985. J. Clin. Microbiol. 44: 4515-4527 [Abstract] [Full Text]  
  • Kilic, A., Li, H., Stratton, C. W., Tang, Y.-W. (2006). Antimicrobial Susceptibility Patterns and Staphylococcal Cassette Chromosome mec Types of, as Well as Panton-Valentine Leukocidin Occurrence among, Methicillin-Resistant Staphylococcus aureus Isolates from Children and Adults in Middle Tennessee. J. Clin. Microbiol. 44: 4436-4440 [Abstract] [Full Text]  
  • Oliveira, D. C., Milheirico, C., de Lencastre, H. (2006). Redefining a Structural Variant of Staphylococcal Cassette Chromosome mec, SCCmec Type VI.. Antimicrob. Agents Chemother. 50: 3457-3459 [Abstract] [Full Text]  
  • McDonald, M., Dougall, A., Holt, D., Huygens, F., Oppedisano, F., Giffard, P. M., Inman-Bamber, J., Stephens, A. J., Towers, R., Carapetis, J. R., Currie, B. J. (2006). Use of a Single-Nucleotide Polymorphism Genotyping System To Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus aureus in Remote Aboriginal Communities.. J. Clin. Microbiol. 44: 3720-3727 [Abstract] [Full Text]  
  • Fortunov, R. M., Hulten, K. G., Hammerman, W. A., Mason, E. O. Jr, Kaplan, S. L. (2006). Community-Acquired Staphylococcus aureus Infections in Term and Near-Term Previously Healthy Neonates. Pediatrics 118: 874-881 [Abstract] [Full Text]  
  • Noto, M. J., Archer, G. L. (2006). A Subset of Staphylococcus aureus Strains Harboring Staphylococcal Cassette Chromosome mec (SCCmec) Type IV Is Deficient in CcrAB-Mediated SCCmec Excision.. Antimicrob. Agents Chemother. 50: 2782-2788 [Abstract] [Full Text]  
  • Oliveira, D. C., Milheirico, C., Vinga, S., de Lencastre, H. (2006). Assessment of allelic variation in the ccrAB locus in methicillin-resistant Staphylococcus aureus clones. J Antimicrob Chemother 58: 23-30 [Abstract] [Full Text]  
  • Noguchi, N., Nakaminami, H., Nishijima, S., Kurokawa, I., So, H., Sasatsu, M. (2006). Antimicrobial Agent of Susceptibilities and Antiseptic Resistance Gene Distribution among Methicillin-Resistant Staphylococcus aureus Isolates from Patients with Impetigo and Staphylococcal Scalded Skin Syndrome.. J. Clin. Microbiol. 44: 2119-2125 [Abstract] [Full Text]  
  • Liao, R. S., Storch, G. A., Buller, R. S., Orscheln, R. C., Mardis, E. R., Armstrong, J. R., Dunne, W. M. Jr. (2006). Blinded Comparison of Repetitive-Sequence PCR and Multilocus Sequence Typing for Genotyping Methicillin-Resistant Staphylococcus aureus Isolates from a Children's Hospital in St. Louis, Missouri.. J. Clin. Microbiol. 44: 2254-2257 [Abstract] [Full Text]  
  • Rupp, J., Fenner, I., Solbach, W., Gieffers, J. (2006). Be Aware of the Possibility of False-Positive Results in Single-Locus PCR Assays for Methicillin-Resistant Staphylococcus aureus.. J. Clin. Microbiol. 44: 2317-2317 [Full Text]  
  • Sabol, K. E, Echevarria, K. L, Lewis, J. S II (2006). Community-Associated Methicillin-Resistant Staphylococcus aureus: New Bug, Old Drugs. The Annals of Pharmacotherapy 40: 1125-1133 [Abstract] [Full Text]  
  • Vivoni, A. M., Diep, B. A., de Gouveia Magalhaes, A. C., Santos, K. R. N., Riley, L. W., Sensabaugh, G. F., Moreira, B. M. (2006). Clonal Composition of Staphylococcus aureus Isolates at a Brazilian University Hospital: Identification of International Circulating Lineages.. J. Clin. Microbiol. 44: 1686-1691 [Abstract] [Full Text]  
  • Cheng, N.-C., Chang, S.-C., Kuo, Y.-S., Wang, J.-L., Tang, Y.-B. (2006). Necrotizing Fasciitis Caused by Methicillin-Resistant Staphylococcus aureus Resulting in Death. A Report of Three Cases. JBJS 88: 1107-1110 [Full Text]  
  • Gonzalez, B. E., Teruya, J., Mahoney, D. H. Jr, Hulten, K. G., Edwards, R., Lamberth, L. B., Hammerman, W. A., Mason, E. O. Jr, Kaplan, S. L. (2006). Venous Thrombosis Associated With Staphylococcal Osteomyelitis in Children. Pediatrics 117: 1673-1679 [Abstract] [Full Text]  
  • Speziali, C. D., Dale, S. E., Henderson, J. A., Vines, E. D., Heinrichs, D. E. (2006). Requirement of Staphylococcus aureus ATP-Binding Cassette-ATPase FhuC for Iron-Restricted Growth and Evidence that It Functions with More than One Iron Transporter. J. Bacteriol. 188: 2048-2055 [Abstract] [Full Text]  
  • King, M. D., Humphrey, B. J., Wang, Y. F., Kourbatova, E. V., Ray, S. M., Blumberg, H. M. (2006). Emergence of community-acquired methicillin-resistant Staphylococcus aureus USA 300 clone as the predominant cause of skin and soft-tissue infections.. ANN INTERN MED 144: 309-317 [Abstract] [Full Text]  
  • Graham, P. L. III, Lin, S. X., Larson, E. L. (2006). A U.S. population-based survey of Staphylococcus aureus colonization.. ANN INTERN MED 144: 318-325 [Abstract] [Full Text]  
  • Chongtrakool, P., Ito, T., Ma, X. X., Kondo, Y., Trakulsomboon, S., Tiensasitorn, C., Jamklang, M., Chavalit, T., Song, J.-H., Hiramatsu, K. (2006). Staphylococcal Cassette Chromosome mec (SCCmec) Typing of Methicillin-Resistant Staphylococcus aureus Strains Isolated in 11 Asian Countries: a Proposal for a New Nomenclature for SCCmec Elements. Antimicrob. Agents Chemother. 50: 1001-1012 [Abstract] [Full Text]  
  • Ramdani-Bouguessa, N., Bes, M., Meugnier, H., Forey, F., Reverdy, M.-E., Lina, G., Vandenesch, F., Tazir, M., Etienne, J. (2006). Detection of Methicillin-Resistant Staphylococcus aureus Strains Resistant to Multiple Antibiotics and Carrying the Panton-Valentine Leukocidin Genes in an Algiers Hospital. Antimicrob. Agents Chemother. 50: 1083-1085 [Abstract] [Full Text]  
  • Koessler, T., Francois, P., Charbonnier, Y., Huyghe, A., Bento, M., Dharan, S., Renzi, G., Lew, D., Harbarth, S., Pittet, D., Schrenzel, J. (2006). Use of Oligoarrays for Characterization of Community-Onset Methicillin-Resistant Staphylococcus aureus.. J. Clin. Microbiol. 44: 1040-1048 [Abstract] [Full Text]  
  • Hsu, L.-Y., Koh, Y.-L., Chlebicka, N. L., Tan, T.-Y., Krishnan, P., Lin, R. T.-P., Tee, N., Barkham, T., Koh, T.-H. (2006). Establishment of ST30 as the Predominant Clonal Type among Community-Associated Methicillin-Resistant Staphylococcus aureus Isolates in Singapore.. J. Clin. Microbiol. 44: 1090-1093 [Abstract] [Full Text]  
  • Strommenger, B., Kehrenberg, C., Kettlitz, C., Cuny, C., Verspohl, J., Witte, W., Schwarz, S. (2006). Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and their relationship to human isolates. J Antimicrob Chemother 57: 461-465 [Abstract] [Full Text]  
  • Sola, C., Cortes, P., Saka, H. A., Cordoba MRSA Collaborative Study Group, , Vindel, A., Bocco, J. L. (2006). Evolution and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Epidemic and Sporadic Clones in Cordoba, Argentina. J. Clin. Microbiol. 44: 192-200 [Abstract] [Full Text]  
  • Vindel, A., Trincado, P., Gomez, E., Cabrera, R., Boquete, T., Sola, C., Valdezate, S., Saez-Nieto, J. A. (2006). Prevalence and Evolution of Methicillin-Resistant Staphylococcus aureus in Spanish Hospitals between 1996 and 2002. J. Clin. Microbiol. 44: 266-270 [Abstract] [Full Text]  
  • Stephens, A. J., Huygens, F., Inman-Bamber, J., Price, E. P., Nimmo, G. R., Schooneveldt, J., Munckhof, W., Giffard, P. M. (2006). Methicillin-resistant Staphylococcus aureus genotyping using a small set of polymorphisms. J Med Microbiol 55: 43-51 [Abstract] [Full Text]  
  • O'Brien, F. G., Coombs, G. W., Pearson, J. C., Christiansen, K. J., Grubb, W. B. (2005). Type V Staphylococcal Cassette Chromosome mec in Community Staphylococci from Australia. Antimicrob. Agents Chemother. 49: 5129-5132 [Abstract] [Full Text]  
  • Kerttula, A.-M., Lyytikainen, O., Vuopio-Varkila, J., Ibrahem, S., Agthe, N., Broas, M., Jagerroos, H., Virolainen, A. (2005). Molecular Epidemiology of an Outbreak Caused by Methicillin-Resistant Staphylococcus aureus in a Health Care Ward and Associated Nursing Home. J. Clin. Microbiol. 43: 6161-6163 [Abstract] [Full Text]  
  • Rihn, J. A., Michaels, M. G., Harner, C. D. (2005). Community-Acquired Methicillin-Resistant Staphylococcus aureus: An Emerging Problem in the Athletic Population. Am J Sports Med 33: 1924-1929 [Abstract] [Full Text]  
  • McAleese, F., Murphy, E., Babinchak, T., Singh, G., Said-Salim, B., Kreiswirth, B., Dunman, P., O'Connell, J., Projan, S. J., Bradford, P. A. (2005). Use of Ribotyping To Retrospectively Identify Methicillin-Resistant Staphylococcus aureus Isolates from Phase 3 Clinical Trials for Tigecycline That Are Genotypically Related to Community-Associated Isolates. Antimicrob. Agents Chemother. 49: 4521-4529 [Abstract] [Full Text]  
  • Takeuchi, F., Watanabe, S., Baba, T., Yuzawa, H., Ito, T., Morimoto, Y., Kuroda, M., Cui, L., Takahashi, M., Ankai, A., Baba, S.-i., Fukui, S., Lee, J. C., Hiramatsu, K. (2005). Whole-Genome Sequencing of Staphylococcus haemolyticus Uncovers the Extreme Plasticity of Its Genome and the Evolution of Human-Colonizing Staphylococcal Species. J. Bacteriol. 187: 7292-7308 [Abstract] [Full Text]  
  • Wisplinghoff, H., Ewertz, B., Wisplinghoff, S., Stefanik, D., Plum, G., Perdreau-Remington, F., Seifert, H. (2005). Molecular Evolution of Methicillin-Resistant Staphylococcus aureus in the Metropolitan Area of Cologne, Germany, from 1984 to 1998. J. Clin. Microbiol. 43: 5445-5451 [Abstract] [Full Text]  
  • Zhang, K., McClure, J.-A., Elsayed, S., Louie, T., Conly, J. M. (2005). Novel Multiplex PCR Assay for Characterization and Concomitant Subtyping of Staphylococcal Cassette Chromosome mec Types I to V in Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 5026-5033 [Abstract] [Full Text]  
  • Ip, M., Yung, R. W. H., Ng, T. K., Luk, W. K., Tse, C., Hung, P., Enright, M., Lyon, D. J. (2005). Contemporary Methicillin-Resistant Staphylococcus aureus Clones in Hong Kong. J. Clin. Microbiol. 43: 5069-5073 [Abstract] [Full Text]  

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