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Journal of Clinical Microbiology, June 2002, p. 2291-2296, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2291-2296.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Expression of the Bundle-Forming Pilus by Enteropathogenic Escherichia coli Strains of Heterologous Serotypes

Juana Gismero-Ordoñez,1 Monique Dall'Agnol,2 Luiz R. Trabulsi,1 and Jorge A. Girón2*

Departamento de Microbiologia, Universidade de São Paulo, and Instituto Butantã, São Paulo, Brazil,1 Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico2

Received 17 December 2001/ Returned for modification 27 January 2002/ Accepted 1 March 2002


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ABSTRACT
 
The production of the bundle-forming pilus subunit BfpA was investigated in 44 enteropathogenic Escherichia coli (EPEC) diarrheal isolates after growth on several conventional bacteriological media. In all, the use of brucella agar supplemented with 4.5 mM sodium bisulfite resulted in a higher detection of BfpA-producing EPEC.


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TEXT
 
Enteropathogenic Escherichia coli (EPEC) is the most important bacterial cause of diarrheal disease in infants 0 to 6 months of age living in developing countries (11, 15, 23). EPEC strains are characterized by the presence of a 92-kb plasmid that codes for a type IV bundle-forming pilus (Bfp) (12) associated with the formation of tight microcolonies on tissue culture cells, a phenotype referred to as the localized adherence pattern, and virulence (3, 8, 29). Antibodies against BfpA were demonstrated to exist in the sera of children with EPEC infections (22, 25). The Bfp contains a structural subunit of 19.5 kDa, termed BfpA or bundlin, and its expression can be manifested by growth of the bacteria on tryptic soy agar supplemented with 5% defibrinated sheep blood in tissue culture media (e.g., Dulbecco's minimal essential medium with Earle salts [DMEM]) or in the course of infection of cultured epithelial cells (9, 12).

The goal of this study was to find alternative bacteriological media suitable for the induction of Bfp expression and thereby to improve the identification of Bfp-producing EPEC. Towards this end, we analyzed the production of Bfp in a collection of 44 well-defined strains belonging to classical EPEC serotypes isolated from the feces of children with diarrheal disease between 1954 and 1993 in Brazil, Congo, Chile, and Mexico (Tables 1 and 2) (6, 17, 26, 28). Within this bacterial collection, 36 strains showed the EPEC adherence factor (EAF)-positive bfpA+ eae+ genotype typical of EPEC (Table 1) and 8 strains showed the EAF-negative bfpA+ eae+ genotype (Table 2) (6, 17, 26, 28, 30). The prototypical EPEC strains B171 (O111:NM) and E2348/69 (O127:H6) were used as positive controls. JPN15, a plasmidless derivative of E2348/69 that does not produce Bfp, was used as negative control (12, 13). First, we studied the production of BfpA in E2348/69 after growth at 37°C in several conventional laboratory solid media, such as eosin-methylene blue (EMB), MacConkey, brucella, and Mueller-Hinton agars, and compared it to that observed in DMEM by immunoblotting as follows. Equal amounts of bacteria were denatured, electrophoresed, and reacted with polyclonal anti-Bfp antibodies as previously described (13, 14, 21). Obvious differences in the levels of expression of BfpA by E2348/69 were noted in the various growth conditions (Fig. 1). Namely, BfpA was highly induced in DMEM, followed by Mueller-Hinton, EMB, and brucella agars. Small amounts of BfpA were detected in Luria-Bertani and MacConkey agars as well. A slight variation in the molecular migration of BfpA was observed when this strain was grown on EMB agar. However, no obvious morphological differences were noted in the Bfp structures produced by this strain on EMB agar (data not shown). It is possible that the BfpA polypeptide undergoes biochemical modification (e.g., amino acid substitutions) when grown in this medium, resulting in a slower migration in sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. Such variability in migration has been attributed to differences in the lengths of the amino acid chains of BfpA subunits of diverse EPEC strains (4, 13). Antigenic heterogeneity in BfpA has also been noted previously (14).


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  		TABLE 1. Production of BfpA by EAF- and bfpA probe-positive EPEC strains in different bacterial growth media and correlation with production of Bfp filaments on HEp-2 cells


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  		TABLE 2. Production of BfpA by EAF probe-negative and bfpA probe-positive EPEC strains in different bacterial growth media and correlation with production of Bfp filaments on HEp-2 cells



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  		FIG. 1. Expression and detection of BfpA after growth on different bacteriological media. E2348/69 was grown on several solid media and adjusted to the same bacterial concentration on each medium, and the proteins were denatured in sodium dodecyl sulfate-polyacrylamide gel electrophoresis sample buffer. Immunoblots were reacted with anti-Bfp antibodies. Mass standards (in kilodaltons) are indicated on the left, and the BfpA pilin subunit is indicated by an arrow on the right. Note the different levels of synthesis of BfpA in the different media and the difference in the mobility of BfpA for bacteria grown on EMB agar. LB, Luria-Bertani; TSAB, tryptic soy agar supplemented with 5% defibrinated sheep blood; Sb, sodium bisulfite; +, positive; +/-, weakly positive.

We then tested all 36 typical (EAF-positive bfpA+) EPEC strains for the production of bundlin on the selected media. Among these, 33 (91%) produced BfpA in DMEM (Table 1). No difference was found between DMEM containing 5% fetal bovine serum (a common component of tissue culture medium) and DMEM alone (data not shown), suggesting that serum factors do not influence BfpA expression. A considerable number of strains did produce BfpA after growth on solid media, such as MacConkey (89%), EMB (83%), Mueller-Hinton (69%), and brucella (64%) agars. These results are particularly interesting since MacConkey and EMB agars are routinely used for the identification of lactose-positive E. coli isolated from diarrheal stools. These media offer the possibility of identifying EPEC directly in stool samples spotted onto colony blots and detecting BfpA by an immunological assay.

Analysis of Bfp production in the different growth media revealed interesting findings. Some strains produced Bfp selectively in some of the media, and differences were also noted within strains of a particular serotype (Tables 1 and 2). Figure 2 shows the variability of BfpA production between serotypes and between strains of the same serotype after growth on brucella agar.



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  		FIG. 2. Detection of BfpA produced by EPEC strains of heterologous serotypes. The indicated EPEC strains were grown on brucella agar, and BfpA was detected as described in the text. Note that strains of the same serotype behaved differently in terms of BfpA production. The levels and sizes of BfpA produced varied among the isolates.

Compared to typical EPEC strains, fewer strains within the EAF-negative group were able to produce BfpA, including those that grew in DMEM. Only 62% of these showed BfpA in DMEM, while 6 (75%), 4 (50%), 4 (50%), and 3 (37%) strains produced BfpA in EMB, MacConkey, brucella, and Mueller-Hinton agars, respectively (Table 2). Similar to the case for strains in the typical group, various profiles of BfpA production were noted for strains in the EAF-negative group. Only isolate number 18 (O142:H6) produced BfpA in all of the media tested. Some isolates that were BfpA negative in DMEM were positive in other media, such as brucella, EMB, and MacConkey agars. Blank et al. (4) identified eight different bfpA alleles: the {alpha} group contains three alleles found in strains of the O55, O86, O111, O119, O127, and O128 serogroups, and the ß group contains five alleles found in strains of the O55, O119, O128, and O142 serogroups and in strains with nontypeable antigens. We have employed strains belonging to these serogroups, but we could not find an association between the serotype and a specific pattern of BfpA expression with the media tested.

It is known that the expression of some virulence factors is affected negatively or positively by carbon dioxide (10, 19, 20). Thus, we tested the production of bundlin after growth under an atmosphere of 5% carbon dioxide. Although this was not the case for all of the isolates, a few of the typical and EAF-negative EPEC strains which produced BfpA in some of the media tested (7 to 17% in both groups) became BfpA negative after growth in the presence of 5% carbon dioxide (Tables 1 and 2). This effect was especially noticeable when EMB agar was employed. However, this was not a general phenomenon, since we also encountered a few strains that became BfpA positive after growth in the carbon dioxide-containing atmosphere.

Detailed analysis of the nutritional and chemical compositions of all of the media employed revealed that neither NaCl, yeast extract, nor beef infusions were key factors required for Bfp production. We noted that sodium bisulfite (a reducing agent present at a concentration of 0.9 mM) was one of the components present in brucella agar but not in the rest of the media tested. Therefore, we investigated whether higher concentrations (two- to fivefold) of sodium bisulfite added to brucella agar or any other medium, such as Mueller-Hinton or EMB agar, would induce BfpA production in the isolates, especially in those of the EAF-negative EPEC group, which contained many BfpA-negative strains. We did not test MacConkey agar supplemented with sodium bisulfite, because it already showed good stimulation of BfpA production alone. E2348/69 produced more bundlin when grown on brucella plus 4.5 mM sodium bisulfite (hereafter designated Bfp agar) than when grown on brucella agar alone (Fig. 1). We could rescue BfpA production in several of the isolates that were repeatedly negative for BfpA in some of the media, including DMEM and brucella agar. In the typical EPEC group, 97% of the strains produced BfpA on Bfp agar compared to only 64% on brucella agar (Table 1). The positive effect of sodium bisulfite on BfpA production was confirmed with Mueller-Hinton agar, as well. An increase was observed in the number of BfpA-producing EPEC isolates compared to that observed for the media without this compound (Table 1). In the EAF-negative EPEC group, we could rescue BfpA production in three isolates when they were grown on Bfp agar (Table 2). To determine whether sodium bisulfite was exerting any metabolic effect, we measured growth curves in the presence and absence of this compound and noted, as expected, that higher (>4.5 mM) concentrations of this chemical had deleterious effects on the bacteria (data not shown). It appears that Bfp agar is a better alternative to DMEM for promoting BfpA production in both typical and EAF-negative EPEC (Tables 1 and 2).

The environmental factors involved in the regulation of BfpA have been studied in two different EPEC strains, namely B171 (O111:NM) and E2348/69 (O127:H6) (9, 27). In strain B171, the presence of glucose enhances BfpA expression whereas the presence of ammonium, but not urea, nitrate, or nitrite, significantly reduces BfpA expression (2, 7, 27, 32). In contrast, the presence of calcium and magnesium ions has been shown to influence the local adherence pattern phenotype and the production of Bfp (2, 7, 27, 32). The observation that sodium bisulfite enhances BfpA expression adds yet another factor to the overall regulation scheme of this pilus. The identification of the true signals that activate Bfp expression in the small intestine of young infants remains an important issue to address.

To visualize Bfp structures, bacterial colonies growing on Bfp agar were harvested and negatively stained for transmission electron microscopy or reacted with anti-Bfp antibodies on glass slides for immunofluorescence (12). Among the strains studied, 34 (94%) typical EPEC and 4 (50%) EAF-negative EPEC produced Bfp structures (data not shown). Typical Bfp rope-like structures produced by two EPEC strains (O55:H6 number 7 and O142:H34 number 21) are shown in Fig. 3. In agreement with the immunoblotting data, the level of production of Bfp structures varied significantly among the strains, and a few of the isolates of both typical and EAF-negative strains failed to produce BfpA. This could be explained by the lack of accessory genes involved in the regulation, export, anchoring, or polymerization of pilins on the bacterium (4, 5, 31, 32). Bortoloni et al. reported that clinical isolates of EPEC serotypes O128ab:H2 and O119:H2 possess large deletions of bfp genes, do not produce Bfp structures, and only adhere to epithelial cells after 6 h of infection (5). Okeke et al. (24) reported that a subset of O119:H2, O128:H2, and O142:H6 strains carry frameshift mutations in perA (a transcriptional activator of Bfp expression [16]), leading to premature truncation. As with bfpA, molecular variations were found in the perA gene.



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  		FIG. 3. Visualization of Bfp structures produced by EPEC O55:H6 (7) (A and C) and O142:H34 (21) (B and D) strains grown on Bfp agar. (A and B) Negative staining of EPEC showing Bfp rope-like structures; (C and D) detection of Bfp fibers produced by EPEC grown on Bfp agar by immunofluorescence. Arrows indicate Bfp structures.

Several immunological, DNA-based (PCR and hybridization with specific DNA probes), and adherence assays have been proposed to detect EPEC strains in epidemiological settings (1, 8, 13, 18, 29). An enzyme-linked immunosorbent assay was reported to detect localized adherent EPEC employing EPEC-specific antibodies (1). Further characterization of these antibodies indicated that they were directed against the Bfp (J. Girón and J. M. Albert, unpublished data). Direct detection of the gene is simple nowadays and more accurate than immunological assays that rely on expression. However, in areas of the world where EPEC is a frequent cause of childhood diarrhea, the use of molecular diagnostic tools and tissue culture cells is unaffordable. Thus, the use of Bfp agar is an alternative for promoting Bfp production and detection by an immunoassay when Bfp production is being investigated in epidemiological settings or in basic research laboratories. Based on the data obtained, we recommend the use of Bfp agar to obtain maximal production of BfpA in most EPEC strains.


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ACKNOWLEDGMENTS
 
We thank Alejandro Ruiz-Tagle for assistance, Isabel Scaletsky for critical discussion, and James B. Kaper for support during preparation of this manuscript.

J. A. Girón thanks Lilia Cedillo (BUAP), Conacyt (Grant 32777-M), and the Fundação de Apoio à Pesquisa do Estado de São Paulo (FAPESP) for support.


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FOOTNOTES
 
* Corresponding author. Mailing address: Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio 76, Complejo de Ciencias, Ciudad Universitaria, C. P. 72000, Puebla, Pue, Mexico. Phone: 52 222 332010. Fax: 52 222 444518. E-mail: jagiron{at}yahoo.com. Back


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Journal of Clinical Microbiology, June 2002, p. 2291-2296, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2291-2296.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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