JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lastovica, A. J.
Right arrow Articles by Fry, B. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lastovica, A. J.
Right arrow Articles by Fry, B. N.

 Previous Article

Journal of Clinical Microbiology, March 2003, p. 1349-1350, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1349-1350.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

LETTER TO THE EDITOR

Molecular Typing of Campylobacter jejuni and Campylobacter coli


   LETTER
Top
 Letter
References
 Letter 
 References 
 
Shi et al. (7) presented data on the development and application of a PCR-based restriction fragment length polymorphism analysis of a 9.6-kb portion of the lipopolysaccharide gene (LG) cluster of Campylobacter jejuni and Campylobacter coli. This proposed molecular subtyping system is novel and has the potential of being directly applicable to epidemiology, prevalence, and other studies. I agree that if, as the authors contend, their proposed genotyping scheme has the same molecular basis as the Penner serotyping scheme (5, 6), then C. jejuni and C. coli isolates previously nontypeable by the Penner scheme are likely to be typeable by the LG scheme.

However, I do not agree that the proposed LG typing scheme is specific for C. jejuni and C. coli only. While C. lari and C. upsaliensis are less frequently isolated from clinical specimens than C. jejuni and C. coli, these organisms have been linked to colitis, bacteremia, spontaneous human abortion, the hemolytic-uremic syndrome, and other illnesses (1, 2, 3). C. lari strains are serotypeable by the Penner system (2, 8; A. J. Lastovica, unpublished data), as are C. upsaliensis strains (2; Lastovica, unpublished). That some strains of C. upsaliensis are serotypeable may reflect the genomic heterogeneity of C. upsaliensis, as has been proposed (4).

Since C. lari and C. upsaliensis are typeable with the Penner system, they also should be typeable by the LG scheme. This question was not adequately addressed, as only a single strain each of C. lari and C. upsaliensis was analyzed by the LG scheme and no PCR product was obtained (7).

Additional LG analysis on a number of serologically defined C. lari and C. upsaliensis strains will resolve this question and extend the usefulness of the proposed LG molecular subtyping scheme.


   REFERENCES
Top
 Letter
 References
Letter 
 References 
 

  1. Bourke, B., V. L. Chan, and P. Sherman. 1998. Campylobacter upsaliensis: waiting in the wings. Clin. Microbiol. Rev. 11:440-449.[Abstract/Free Full Text]
  2. Lastovica, A. J., E. Le Roux, and J. L. Penner. 1989. "Campylobacter upsaliensis" isolated from blood cultures of pediatric patients. J. Clin. Microbiol. 27:657-659.[Abstract/Free Full Text]
  3. Lastovica, A. J., and M. B. Skirrow. 2000. Clinical significance of Campylobacter and related species other than C. jejuni and C. coli, p 89-120. In I. Nachamkin and M. J. Blaser (ed.), Campylobacter, 2nd ed. American Society for Microbiology, Washington, D.C.
  4. Moser, I., B. Rieksneuwöhner, P. Lentzsch, P. Schwerk, and L. H. Wieler. 2001. Genomic heterogeneity and O-antigenic diversity of Campylobacter upsaliensis and Campylobacter helveticus strains isolated from dogs and cats in Germany. J. Clin. Microbiol. 39:2548-2557.[Abstract/Free Full Text]
  5. Penner, J. L., and J. N. Hennessey. 1980. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J. Clin. Microbiol. 12:732-737.[Abstract/Free Full Text]
  6. Penner, J. L., J. N. Hennessy, and R. V. Congi. 1983. Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. Eur. J. Clin. Microbiol. 2:378-383.[CrossRef][Medline]
  7. Shi, F., Y. Y. Chen, T. M. Wassenaar, W. H. Woods, P. J. Coloe, and B. N. Fry. 2002. Development and application of a new scheme for typing Campylobacter jejuni and Campylobacter coli by PCR-based restriction fragment length polymorphism analysis. J. Clin. Microbiol. 40:1791-1797.[Abstract/Free Full Text]
  8. Woodward, D. L., and F. G. Rodgers. 2002. Identification of Campylobacter heat-stable and heat-labile antigens by combining the Penner and Lior serotyping schemes. J. Clin. Microbiol. 40:741-745.[Free Full Text]
Albert J. Lastovica
Department of Medical Microbiology
University of Cape Town
Cape Town, South Africa


Authors' Reply


   LETTER 
Top
 Letter
 References
 Letter 
References 
 
We are most grateful to Professor Albert Joseph Lastovica for his interest in our recent paper on the development of a novel Campylobacter genotyping scheme. Before we comment on the specificity of the LG typing scheme, we should clarify the current molecular understanding of the Penner and LG typing methods.

The molecular basis for the Penner heat-stable antigenic diversity in C. jejuni and C. coli was thought to be the expression of somatic (O) lipopolysaccharide (LPS) (3, 4, 5, 6, 8, 9, 10). LPS is a major constituent of the outer membrane in gram-negative bacteria and comprises three covalently linked regions: lipid A, core oligosaccharide (inner core and outer core), and O polysaccharide. The variability of the Campylobacter LPS outer core and O polysaccharide was thought to contribute to the antigenic basis of the Penner serotyping system. However, recent evidence showed that the high-molecular-weight O polysaccharide is a capsular polysaccharide which is not linked to the LPS molecule of C. jejuni and which accounted in some strains for the Penner serotype specificity (2, 13). This evidence also indicated that C. jejuni strains carry lipooligosaccharide (LOS) molecules instead of LPS molecules. In addition, Moran et al. (6a) and Oza et al. (7) showed that this LOS is part of the heat-stable antigens, which contribute to passive hemagglutination and direct agglutination.

Part of the LOS biosynthesis gene cluster (wla) of C. jejuni 81116 has recently been characterized in our laboratory (1). The LOS gene (LG) typing scheme was established by PCR-restriction fragment length polymorphism of the wla gene cluster (11). Some of the genes in the same cluster were shown to be involved in protein glycosylation in C. jejuni strain 81-176 (12). Therefore, the LG typing scheme should be renamed the LOS/glycosylation typing scheme.

Given the background of both systems, we can now address the question of the specificity of the LG typing system. Although some of the C. lari and C. upsaliensis strains may share some common surface epitopes (capsule or LOS) and can be typed by the Penner serotyping system, they may still have distinct wla gene clusters (different gene elements and organization). Furthermore, the galE and wlaH genes in the wla clusters from C. lari and C. upsaliensis strains may not be highly similar to those of C. jejuni and C. coli. Therefore, the primers galE1 and wlaH3 would be unable to bind to these genes and would not give a PCR product. However, in all tested C. jejuni and C. coli strains the galE and wlaH genes are present and highly conserved and therefore all these strains are 100% typeable with the LG system. We agree with Professor Lastovica's suggestion that additional LG analysis on a number of serologically defined C. lari and C. upsaliensis strains will help to further confirm the specificity of the LG typing scheme. In addition, genetic analysis of the wla gene clusters from C. lari and C. upsaliensis strains will show why the LG system is specific for C. jejuni and C. coli strains.


   REFERENCES 
Top
 Letter
 References
 Letter 
 References 
 

  1. Fry, B. N., V. Korolik, J. A. ten Brinke, M. T. T. Pennings, R. Zalm, B. J. J. Teunis, P. J. Coloe, and B. A. M. van der Zeijst. 1998. The lipopolysaccharide biosynthesis locus of Campylobacter jejuni 81116. Microbiology 144:2049-2061.[Abstract]
  2. Karlyshev, A. V., D. Linton, N. A. Gregson, A. J. Lastovica, and B. W. Wren. 2000. Genetic and biochemical evidence of a Campylobacter jejuni capsular polysaccharide that accounts for Penner serotype specificity. Mol. Microbiol. 35:529-541.[CrossRef][Medline]
  3. Mandatori, R., and J. L. Penner. 1989. Structural and antigenic properties of Campylobacter coli. Infect. Immun. 57:3506-3511.[Abstract/Free Full Text]
  4. Mills, S. D., W. C. Bradbury, and J. L. Penner. 1985. Basis for serological heterogeneity of thermostable antigens of Campylobacter jejuni. Infect. Immun. 50:284-291.[Abstract/Free Full Text]
  5. Moran, A. P., and J. L. Penner. 1999. Serotyping of Campylobacter jejuni based on heat-stable antigens: relevance, molecular basis and implications in pathogenesis. J. Appl. Microbiol. 86:361-377.[CrossRef][Medline]
  6. Moran, A. P., and T. U. Kosunen. 1989. Serological analysis of the heat-stable antigens involved in serotyping Campylobacter jejuni and Campylobacter coli. APMIS 97:253-260.[Medline]
  7. Moran, A. P., T. U. Kosunen, and M. M. Prendergast. 2001. Biochemical and serological analysis of the heat-stable antigens involved in serotyping Campylobacter jejuni/coli. Int. J. Med. Microbiol. 291(Suppl. 31):72-73.
  8. Oza, A. N., R. T. Thwaites, D. R. A. Wareing, F. J. Bolton, and J. A. Frost. 2002. Detection of heat-stable antigens of Campylobacter jejuni and C. coli by direct agglutination and passive hemagglutination. J. Clin. Microbiol. 40:996-1000.[Abstract/Free Full Text]
  9. Perez-Perez, G. I., and M. J. Blaser. 1985. Lipopolysaccharide characteristics of pathogenic campylobacters. Infect. Immun. 47:353-359.[Abstract/Free Full Text]
  10. Perez-Perez, G. I., J. A. Hopkins, and M. J. Blaser. 1985. Antigenic heterogeneity of lipopolysaccharides from Campylobacter jejuni and Campylobacter fetus. Infect. Immun. 48:528-533.[Abstract/Free Full Text]
  11. Preston, M. A., and J. L. Penner. 1987. Structural and antigenic properties of lipopolysaccharides from serotype reference strains of Campylobacter jejuni. Infect. Immun. 55:1806-1812.[Abstract/Free Full Text]
  12. Shi, F., Y. Y. Chen, T. M. Wassenaar, W. H. Woods, P. J. Coloe, and B. N. Fry. 2002. Development and application of a new scheme for typing Campylobacter jejuni and Campylobacter coli by PCR-based restriction fragment length polymorphism analysis. J. Clin. Microbiol. 40:1791-1797.
  13. Szymanski, C. M., R. Yao, C. P. Ewing, T. J. Trust, and P. Guerry. 1999. Evidence for a system of general protein glycosylation in Campylobacter jejuni. Mol. Microbiol. 32:1022-1030.[CrossRef][Medline]
  14. Wren, B. W., D. Linton, N. Dorrell, and A. V. Karlyshev. 2001. Post genome analysis of Campylobacter jejuni. J. Appl. Microbiol. 90:36S-44S.[CrossRef]
Feng Shi
Benjamin N. Fry
Department of Biotechnology and Environmental Biology
Royal Melbourne Institute of Technology University
Melbourne, Australia

Journal of Clinical Microbiology, March 2003, p. 1349-1350, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1349-1350.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lastovica, A. J.
Right arrow Articles by Fry, B. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lastovica, A. J.
Right arrow Articles by Fry, B. N.

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS