TABLE 1. Phenotypic and genotypic properties of MRSA strains from different inpatients at the University Hospital of Patras, Patras, Greece
| Clonal typea (ClaI-mecA type:: Tn554 type:: PFGE type) |
Antibiotic resistanceb |
Oxacillin MIC (mg/liter) |
Yr of isola- tion |
No. (%) of isolates |
spaA type |
Allelic profiled |
ST |
SCCmec type |
|
| VII'::NH::A |
Amp, Oxa, Amc, Ipm |
4-256 |
1993 |
7 |
WGKAKAOMQQQ |
|
|
IV variante |
|
Amp, Oxa, Amc, Ipm |
64-1,024 |
1998 |
14 |
WGKAKAOMQQQ |
2-2-2-2-6-3-2 |
30 |
IV variante |
|
Amp, Oxa, Amc, Ipm |
64-1,024 |
1999 |
8 |
WGKAKAOMQQQQ |
2-2-2-2-6-3-2 |
30 |
IV variante |
|
Amp, Oxa, Amc, Ipm |
 1,024 |
2000 |
5 |
WGKAKAOMQQQ |
|
|
IV variante |
| Total PFGE type A |
|
|
|
34 (29) |
|
|
|
|
| X'::KK::B |
Amp, Oxa, Amc, Ipm, Ery, Cip, Fd, Sxt |
128 |
1998 |
13 |
WGKAOMQ |
2-3-1-1-4-4-3 |
239 |
IIIA |
|
Amp, Oxa, Amc, Ipm, Ery, Cip, Fd, Sxt |
>1,024 |
1999 |
12 |
XKAOMQ |
2-3-1-1-4-4-3 |
239 |
IIIA |
|
Amp, Oxa, Amc, Ipm, Ery, Cip, Fd, Sxt |
1,024 |
2000 |
16 |
XKAOMQ |
|
|
IIIA |
| III'::KK::B |
Amp, Oxa, Amc, Ipm, Ery, Cip, Fd, Sxt |
128 |
1998 |
11 |
WGKAOMQ |
2-3-1-1-4-4-3 |
239 |
III |
|
Amp, Oxa, Amc, Ipm, Ery, Cip, Fd, Sxt |
1,024 |
1999 |
9 |
WGKAOMQ |
|
|
III |
|
Amp, Oxa, Amc, Ipm, Ery, Cip, Fd, Sxt |
>1,024 |
2000 |
3 |
NDf |
|
|
III |
| III::KK::B |
Amp, Oxa, Amc, Ipm, Cip, Fd, Sxt, Amk |
128-256 |
1993 |
2 |
WGKAOMQ |
|
|
III |
|
Amp, Oxa, Amc, Ipm, Ery, Cip, Fd, Sxtc |
1,024 |
1999 |
2 |
WGKAOMQ |
|
|
III |
|
Amp, Oxa, Amc, Ipm, Ery, Cip, Fd, Sxt |
>1,024 |
2000 |
3 |
WGKAOMQ |
|
|
III |
| Total PFGE type B |
|
|
|
71 (60) |
|
|
|
|
| II::NH::C |
Amp, Oxa, Amc, Ipm, Ery |
64 |
1998 |
1 |
UJGBBPB |
1-3-1-14-11-51-10 |
80 |
IV |
|
Amp, Oxa, Amc, Ipm, Fd |
32 |
2000 |
10 |
UJGBBPB |
1-3-1-14-11-51-10 |
80 |
IV |
| Total PFGE type C |
|
|
|
11 (9) |
|
|
|
|
| II::E::D |
Amp, Oxa, Amc, Ipm, Ery, Sxt, Spc |
8 |
1993 |
1 |
YGFMBQBLPO |
3-3-1-1-4-4-3 |
8 |
IV |
| III::B::E |
Amp, Oxa, Amc, Ipm, Ery, Sxt |
16 |
1998 |
1 |
XKAOMMQ |
2-3-1-1-4-4-3 |
239 |
III |
|
a The ClaI-mecA::Tn554 polymorphs molecular sizes of the respective hybridization fragments were as follows: II, III (17), and III' (2.2 and 4.05 kb); VII' (2.2 and 7.6 kb); and X' (2.2 and 5.9 kb). The Tn554 polymorphs were as follows: NH, no homology (lack of transposon); B and E (17); and KK, novel pattern (7.4 kb).
b The panel of antimicrobials included ampicillin (Amp), oxacillin (Oxa), amoxicillin clavulanic acid (Amc), imipenem (Ipm), erythromycin (Ery), ciprofloxacin (Cip), fusidic acid (Fd), sulfamethoxazole-trimethoprim (Sxt), amikacin (Amk), and spectinomycin (Spc).
c One isolate was susceptible to erythromycin, ciprofloxacin, fusidic acid, and sulfamethoxazole-trimethoprim.
d Allelic profile assignment (arcC-aroE-glpF-gmk-pta-tpi-yqiL) (10).
e The SCCmec type was determined by PCR amplification of the ccr gene (13, 21) and the mec gene complex (21) when the SCCmec type could not be inferred by the multiplex PCR strategy (24).
f ND, not determined.
