This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coenye, T.
Right arrow Articles by Mahenthiralingam, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coenye, T.
Right arrow Articles by Mahenthiralingam, E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2003, p. 2797-2798, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2797-2798.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

LETTER TO THE EDITOR

Updated Version of the Burkholderia cepacia Complex Experimental Strain Panel


arrow
LETTER
 
The Burkholderia cepacia complex consists of nine closely related species: Burkholderia cepacia genomovars I and VI, Burkholderia multivorans (genomovar II), Burkholderia cenocepacia (genomovar III), Burkholderia stabilis (genomovar IV), Burkholderia vietnamiensis (genomovar V), Burkholderia ambifaria (genomovar VII), Burkholderia anthina (genomovar VIII), and Burkholderia pyrrocinia (genomovar IX) (2, 9, 10). Despite the advances that have been made in the understanding of the taxonomy and epidemiology of this group, infections of the respiratory tract with B. cepacia complex organisms still have a considerable impact on morbidity and mortality in cystic fibrosis (CF) patients (4, 5, 6). B. cepacia complex strains have also attracted attention as possible biocontrol and bioremediation agents that could be used as antagonists of soil-borne plant pathogens, as plant growth-promoting agents, and for the bioremediation of various recalcitrant xenobiotics (3, 8). To aid in identification, epidemiological tracking, and virulence studies, a set of strains representative of the first five known genomovars of the B. cepacia complex was assembled (7). A review of the literature reveals that this panel has been used extensively in various areas of research (for a complete list of publications using this panel, see http://go.to/cepacia and follow the link to B. cepacia experimental strain panel). Since its assembly, however, several additional species within the B. cepacia complex have been described, and therefore we propose to update the previously described panel.

Strains were selected such that they represent the currently known diversity in isolation sources and geography and the intraspecies diversity. The strains included in the expanded panel (Table 1) were cultured as described previously (7). All isolates were identified to the species level using a polyphasic approach (2). To avoid the inclusion of members of the same clone, all strains were typed using previously described typing methods (1).


View this table:
[in this window]
[in a new window]
  		TABLE 1. Update of the B. cepacia complex strain panel

Molecular typing showed that all strains had clearly different fingerprint patterns.

Four B. cepacia genomovar VI strains are included in the panel. Strains CEP021 and AU0645 were isolated from CF patients in the United States, while E12 was isolated from a CF patient in the United Kingdom. Strain STM1441 was isolated from the rhizosphere of Alysicarpus glumaceus in Senegal.

Three B. ambifaria strains are included in the panel. DNA-based fingerprinting showed that all possessed a unique genetic fingerprint. The type strain of B. ambifaria, AMMDT, was isolated from the rhizosphere of peas (Pisum sativum L.) in the United States. It is one of the most studied biocontrol isolates. B. ambifaria ATCC 53266 is another strain with possible biocontrol applications; it was isolated from corn roots in the United States. Strain CEP0996 was isolated from the sputum of a CF patient in Australia.

Four B. anthina strains are included. The type strain W92T was isolated from the rhizosphere of a houseplant in Nashville, Tenn., in 1997, while strain C1765 was isolated from the respiratory tract of a CF patient in the United Kingdom. J2552 was isolated from the rhizosphere of Carludaucas palmata in the tropical aquatic house of a botanic garden in the United Kingdom. Isolate AU1293 was isolated from the respiratory tract of a CF patient in the United States; this patient has been chronically infected with this organism for over 2 years.

Four B. pyrrocinia isolates are included in the panel. The type strain ATCC 15958T was isolated from soil. B. pyrrocinia ATCC 39277 was isolated from cornfield soil in the United States. Isolate BC011 was isolated from a blackwater stream the United States. Isolate C1469 was isolated from a CF patient attending a treatment center in the United Kingdom.

All strains were deposited in and can be obtained from the BCCM/LMG Bacteria Collection, Ghent, Belgium. On the website of the International Burkholderia cepacia Working Group (http://go.to/cepacia), all available information on the updated B. cepacia complex experimental strain panel will be compiled.


arrow
REFERENCES
 
    1
  1. Coenye, T., T. Spilker, A. Martin, and J. J. LiPuma. 2002. Comparative assessment of genotyping methods for epidemiologic study of Burkholderia cepacia genomovar III. J. Clin. Microbiol. 40:3300-3307.[Abstract/Free Full Text]
    2. 2
  2. Coenye, T., P. Vandamme, J. R. W. Govan, and J. J. LiPuma. 2001. Taxonomy and identification of the Burkholderia cepacia complex. J. Clin. Microbiol. 39:3427-3436.[Free Full Text]
    3. 3
  3. Holmes, A., J. Govan, and R. Goldstein. 1998. Agricultural use of Burkholderia (Pseudomonas) cepacia: a threat to human health? Emerg. Infect. Dis. 4:221-227.[Medline]
    4. 4
  4. Jones, A. M., M. E. Dodd, and A. K. Webb. 2001. Burkholderia cepacia: current clinical issues, environmental controversies and ethical dilemmas. Eur. Respir. J. 17:295-301.[Abstract/Free Full Text]
    5. 5
  5. LiPuma, J. J. 1998. Burkholderia cepacia: management issues and new insights. Clin. Chest Med. 19:473-486.[CrossRef][Medline]
    6. 6
  6. Mahenthiralingam, E., A. Baldwin, and P. Vandamme. 2002. Burkholderia cepacia complex infection in patients with cystic fibrosis. J. Med. Microbiol. 51:533-538.[Abstract/Free Full Text]
    7. 7
  7. Mahenthiralingam, E., T. Coenye, J. W. Chung, D. P. Speert, J. R. W. Govan, P. Taylor, and P. Vandamme. 2000. Diagnostically and experimentally useful panel of strains from the Burkholderia cepacia complex. J. Clin. Microbiol. 38:910-913.[Abstract/Free Full Text]
    8. 8
  8. Parke, J. L., and D. Gurian-Sherman. 2001. Diversity of Burkholderia cepacia complex and implications for risk assessment of biological control strains. Annu. Rev. Phytopathol. 39:225-258.[CrossRef][Medline]
    9. 9
  9. Vandamme, P., D. Henry, T. Coenye, S. Nzula, M. Vancanneyt, J. J. LiPuma, D. P. Speert, J. R. W. Govan, and E. Mahenthiralingam. 2002. Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound test results of new molecular diagnostic tools. FEMS Immunol. Med. Microbiol. 33:143-149.[CrossRef][Medline]
    10. 10
  10. Vandamme, P., B. Holmes, T. Coenye, J. Goris, E. Mahenthiralingam, J. J. LiPuma, and J. R. W. Govan. 2003. Burkholderia cenocepacia sp. nov., a new twist of an old story. Res. Microbiol. 154:91-96.[Medline]
Tom Coenye*
Peter Vandamme
Laboratorium voor Microbiologie
Ghent University
K. L. Ledganckstraat 35
B-9000 Ghent, Belgium

John J. LiPuma
Department of Pediatrics and Communicable Diseases
University of Michigan
Ann Arbor, Michigan

John R. W. Govan
Department of Medical Microbiology
University of Edinburgh
Edinburgh, United Kingdom

Eshwar Mahenthiralingam
School of Biosciences
Cardiff University
Cardiff, United Kingdom

* Phone: 32 9 264 51 14
Fax: 32 9 264 50 92
E-mail: Tom.Coenye{at}rug.ac.be

Journal of Clinical Microbiology, June 2003, p. 2797-2798, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2797-2798.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Uehlinger, S., Schwager, S., Bernier, S. P., Riedel, K., Nguyen, D. T., Sokol, P. A., Eberl, L. (2009). Identification of Specific and Universal Virulence Factors in Burkholderia cenocepacia Strains by Using Multiple Infection Hosts. Infect. Immun. 77: 4102-4110 [Abstract] [Full Text]  
  • Kooi, C., Sokol, P. A. (2009). Burkholderia cenocepacia zinc metalloproteases influence resistance to antimicrobial peptides. Microbiology 155: 2818-2825 [Abstract] [Full Text]  
  • Seed, K. D., Dennis, J. J. (2009). Experimental Bacteriophage Therapy Increases Survival of Galleria mellonella Larvae Infected with Clinically Relevant Strains of the Burkholderia cepacia Complex. Antimicrob. Agents Chemother. 53: 2205-2208 [Abstract] [Full Text]  
  • Malott, R. J., O'Grady, E. P., Toller, J., Inhulsen, S., Eberl, L., Sokol, P. A. (2009). A Burkholderia cenocepacia Orphan LuxR Homolog Is Involved in Quorum-Sensing Regulation. J. Bacteriol. 191: 2447-2460 [Abstract] [Full Text]  
  • Rose, H., Baldwin, A., Dowson, C. G., Mahenthiralingam, E. (2009). Biocide susceptibility of the Burkholderia cepacia complex. J Antimicrob Chemother 63: 502-510 [Abstract] [Full Text]  
  • McClean, S., Callaghan, M. (2009). Burkholderia cepacia complex: epithelial cell-pathogen confrontations and potential for therapeutic intervention. J Med Microbiol 58: 1-12 [Abstract] [Full Text]  
  • Bartholdson, S. J., Brown, A. R., Mewburn, B. R., Clarke, D. J., Fry, S. C., Campopiano, D. J., Govan, J. R. W. (2008). Plant host and sugar alcohol induced exopolysaccharide biosynthesis in the Burkholderia cepacia complex. Microbiology 154: 2513-2521 [Abstract] [Full Text]  
  • Vial, L., Lepine, F., Milot, S., Groleau, M.-C., Dekimpe, V., Woods, D. E., Deziel, E. (2008). Burkholderia pseudomallei, B. thailandensis, and B. ambifaria Produce 4-Hydroxy-2-Alkylquinoline Analogues with a Methyl Group at the 3 Position That Is Required for Quorum-Sensing Regulation. J. Bacteriol. 190: 5339-5352 [Abstract] [Full Text]  
  • Jacobs, J. L., Fasi, A. C., Ramette, A., Smith, J. J., Hammerschmidt, R., Sundin, G. W. (2008). Identification and Onion Pathogenicity of Burkholderia cepacia Complex Isolates from the Onion Rhizosphere and Onion Field Soil. Appl. Environ. Microbiol. 74: 3121-3129 [Abstract] [Full Text]  
  • Zlosnik, J. E. A., Hird, T. J., Fraenkel, M. C., Moreira, L. M., Henry, D. A., Speert, D. P. (2008). Differential Mucoid Exopolysaccharide Production by Members of the Burkholderia cepacia Complex. J. Clin. Microbiol. 46: 1470-1473 [Abstract] [Full Text]  
  • Seed, K. D., Dennis, J. J. (2008). Development of Galleria mellonella as an Alternative Infection Model for the Burkholderia cepacia Complex. Infect. Immun. 76: 1267-1275 [Abstract] [Full Text]  
  • Flannagan, R. S., Valvano, M. A. (2008). Burkholderia cenocepacia requires RpoE for growth under stress conditions and delay of phagolysosomal fusion in macrophages. Microbiology 154: 643-653 [Abstract] [Full Text]  
  • Lynch, K. H., Dennis, J. J. (2008). Development of a Species-Specific fur Gene-Based Method for Identification of the Burkholderia cepacia Complex. J. Clin. Microbiol. 46: 447-455 [Abstract] [Full Text]  
  • Baldwin, A., Mahenthiralingam, E., Drevinek, P., Pope, C., Waine, D. J., Henry, D. A., Speert, D. P., Carter, P., Vandamme, P., LiPuma, J. J., Dowson, C. G. (2008). Elucidating Global Epidemiology of Burkholderia multivorans in Cases of Cystic Fibrosis by Multilocus Sequence Typing. J. Clin. Microbiol. 46: 290-295 [Abstract] [Full Text]  
  • Mendes, R., Pizzirani-Kleiner, A. A., Araujo, W. L., Raaijmakers, J. M. (2007). Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates. Appl. Environ. Microbiol. 73: 7259-7267 [Abstract] [Full Text]  
  • Caraher, E. M., Gumulapurapu, K., Taggart, C. C., Murphy, P., McClean, S., Callaghan, M. (2007). The effect of recombinant human lactoferrin on growth and the antibiotic susceptibility of the cystic fibrosis pathogen Burkholderia cepacia complex when cultured planktonically or as biofilms. J Antimicrob Chemother 60: 546-554 [Abstract] [Full Text]  
  • Brown, A. R., Govan, J. R. W. (2007). Assessment of Fluorescent In Situ Hybridization and PCR-Based Methods for Rapid Identification of Burkholderia cepacia Complex Organisms Directly from Sputum Samples. J. Clin. Microbiol. 45: 1920-1926 [Abstract] [Full Text]  
  • Kooi, C., Subsin, B., Chen, R., Pohorelic, B., Sokol, P. A. (2006). Burkholderia cenocepacia ZmpB Is a Broad-Specificity Zinc Metalloprotease Involved in Virulence. Infect. Immun. 74: 4083-4093 [Abstract] [Full Text]  
  • Loutet, S. A., Flannagan, R. S., Kooi, C., Sokol, P. A., Valvano, M. A. (2006). A Complete Lipopolysaccharide Inner Core Oligosaccharide Is Required for Resistance of Burkholderia cenocepacia to Antimicrobial Peptides and Bacterial Survival In Vivo. J. Bacteriol. 188: 2073-2080 [Abstract] [Full Text]  
  • Wopperer, J., Cardona, S. T., Huber, B., Jacobi, C. A., Valvano, M. A., Eberl, L. (2006). A Quorum-Quenching Approach To Investigate the Conservation of Quorum-Sensing-Regulated Functions within the Burkholderia cepacia Complex. Appl. Environ. Microbiol. 72: 1579-1587 [Abstract] [Full Text]  
  • Kenna, D. T., Yesilkaya, H., Forbes, K. J., Barcus, V. A., Vandamme, P., Govan, J. R. W. (2006). Distribution and genomic location of active insertion sequences in the Burkholderia cepacia complex. J Med Microbiol 55: 1-10 [Abstract] [Full Text]  
  • Whitby, P. W., VanWagoner, T. M., Taylor, A. A., Seale, T. W., Morton, D. J., LiPuma, J. J., Stull, T. L. (2006). Identification of an RTX determinant of Burkholderia cenocepacia J2315 by subtractive hybridization. J Med Microbiol 55: 11-21 [Abstract] [Full Text]  
  • Gingues, S., Kooi, C., Visser, M. B., Subsin, B., Sokol, P. A. (2005). Distribution and Expression of the ZmpA Metalloprotease in the Burkholderia cepacia Complex. J. Bacteriol. 187: 8247-8255 [Abstract] [Full Text]  
  • Mahenthiralingam, E, Vandamme, P (2005). Taxonomy and pathogenesis of the Burkholderia cepacia complex. Chronic Respiratory Disease 2: 209-217 [Abstract]  
  • Baldwin, A., Mahenthiralingam, E., Thickett, K. M., Honeybourne, D., Maiden, M. C. J., Govan, J. R., Speert, D. P., LiPuma, J. J., Vandamme, P., Dowson, C. G. (2005). Multilocus Sequence Typing Scheme That Provides Both Species and Strain Differentiation for the Burkholderia cepacia Complex. J. Clin. Microbiol. 43: 4665-4673 [Abstract] [Full Text]  
  • Bernier, S. P., Sokol, P. A. (2005). Use of Suppression-Subtractive Hybridization To Identify Genes in the Burkholderia cepacia Complex That Are Unique to Burkholderia cenocepacia. J. Bacteriol. 187: 5278-5291 [Abstract] [Full Text]  
  • Payne, G. W., Vandamme, P., Morgan, S. H., LiPuma, J. J., Coenye, T., Weightman, A. J., Jones, T. H., Mahenthiralingam, E. (2005). Development of a recA Gene-Based Identification Approach for the Entire Burkholderia Genus. Appl. Environ. Microbiol. 71: 3917-3927 [Abstract] [Full Text]  
  • Kooi, C., Corbett, C. R., Sokol, P. A. (2005). Functional Analysis of the Burkholderia cenocepacia ZmpA Metalloprotease. J. Bacteriol. 187: 4421-4429 [Abstract] [Full Text]  
  • Ramette, A., LiPuma, J. J., Tiedje, J. M. (2005). Species Abundance and Diversity of Burkholderia cepacia Complex in the Environment. Appl. Environ. Microbiol. 71: 1193-1201 [Abstract] [Full Text]  
  • Vinion-Dubiel, A. D., Spilker, T., Dean, C. R., Monteil, H., LiPuma, J. J., Goldberg, J. B. (2004). Correlation of wbiI Genotype, Serotype, and Isolate Source within Species of the Burkholderia cepacia Complex. J. Clin. Microbiol. 42: 4121-4126 [Abstract] [Full Text]  
  • Weaver, V. B., Kolter, R. (2004). Burkholderia spp. Alter Pseudomonas aeruginosa Physiology through Iron Sequestration. J. Bacteriol. 186: 2376-2384 [Abstract] [Full Text]  
  • Baldwin, A., Sokol, P. A., Parkhill, J., Mahenthiralingam, E. (2004). The Burkholderia cepacia Epidemic Strain Marker Is Part of a Novel Genomic Island Encoding Both Virulence and Metabolism-Associated Genes in Burkholderia cenocepacia. Infect. Immun. 72: 1537-1547 [Abstract] [Full Text]  

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coenye, T.
Right arrow Articles by Mahenthiralingam, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coenye, T.
Right arrow Articles by Mahenthiralingam, E.

Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»