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Journal of Clinical Microbiology, September 2003, p. 4492, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4492.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

LETTER TO THE EDITOR

Presence of Brachyspira aalborgi and B. pilosicoli in Feces of Patients with Diarrhea


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LETTER
 
Anaerobic intestinal spirochetes of the genus Brachyspira are important enteric pathogens in animals, but they have not been extensively studied in humans. The presence of a layer of spirochetes attached by one cell end to the colorectal epithelium is known as intestinal spirochetosis (IS) (4). The clinical significance of this colonization is uncertain, but it has been linked to chronic diarrhea and other abdominal complaints (3). Two species, Brachyspira pilosicoli and Brachyspira aalborgi, have been associated with human IS (7).

In a study of Dutch patients with gastroenteritis, DNA was extracted from feces by using guanidinium thiocyanate and silica and used for PCR detection of enteric pathogens (2). In 61% of cases, no known etiologic agent could be detected. Here, DNA samples from a subset of 182 of these samples, of which 89 came from patients with chronic diarrhea and 93 from patients with short-term diarrhea (less than 15 days) and in which no other pathogen could be detected, were subjected to PCR based upon the 16S rDNA sequences of B. aalborgi and B. pilosicoli (8).

No sample was PCR positive for B. pilosicoli, but two (1.1%), one from each category, were positive for B. aalborgi. Patient 1 was a 21-year-old female with abdominal pain and cramps, with frequent stools containing mucus. Symptoms had persisted for 21 days. The patient had traveled to Turkey and Jordan in the week before the onset of symptoms. She owned a cat and kept poultry. Patient 2 was a 34-year-old female with abdominal pain and cramps, frequent stools, and vomiting. Symptoms had persisted for 9 days. The patient had drunk raw milk in the week before onset. She owned a cat and a rabbit. Both patients had another person in the household with diarrhea and vomiting in the week before the onset of symptoms.

The overall prevalence of B. aalborgi that was found (1.1%) was generally consistent with results for histologic prevalence studies of intestinal spirochetes in biopsies from patients in Western countries (6), apart from presence in human immunodeficiency virus-positive patients and homosexual males, who are commonly (~30 to 50%) colonized by B. pilosicoli (10). The prevalences found may have been underestimated as a result of using a suboptimal method for DNA extraction and/or because of the relatively poor detection limits of the PCRs on feces (8). Nevertheless, the study suggests that intestinal spirochetes are not common in the feces of Western patients who have diarrhea but no other identified pathogens. The relationship between fecal shedding of intestinal spirochetes, diarrhea, and histologic IS remains unknown.

There were several potential sources of infection for the patients. Patient 1 may have become infected while traveling overseas. The prevalence of B. aalborgi in Middle Eastern countries has not been investigated, although carriage rates for B. pilosicoli of ~27% have been found in Oman (1). Both patients had contact with various animal species. While B. aalborgi has only previously been isolated from humans and nonhuman primates (5, 9), intestinal spirochetes have been observed in the feces of many animal species. Unfortunately, because only two colonized individuals were identified, no reliable epidemiological analysis of potential risk factors could be made. Further studies are warranted.


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  2. de Wit, M. A. S., M. P. G. Koopmans, L. M. Kortbeek, N. J. van Leeuwen, A. I. M. Bartelds, and Y. T. H. P. van Duynhoven. 2001. Gastroenteritis in sentinel general practices, The Netherlands. Emerg. Infect. Dis. 7:82-91.[Medline]
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  3. Gad, A., R. Willen, K. Furugard, B. Fors, and M. Hradsky. 1997. Intestinal spirochaetosis as a cause of long standing diarrhoea. Ups. J. Med. Sci. 82:49-54.
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  5. Hovind-Hougen, K., A. Birch-Andersen, R. Henrik-Nielsen, M. Orholm, J. O. Pedersen, P. S. Teglbjærg, and E. H. Thaysen. 1982. Intestinal spirochetosis: morphological characterization and cultivation of the spirochete Brachyspira aalborgi gen. nov., sp. nov. J. Clin. Microbiol. 16:1127-1136.[Abstract/Free Full Text]
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  6. Lindboe, C. F. 2001. The prevalence of human intestinal spirochetosis in Norway. Anim. Health Res. Rev. 2:117-119.[Medline]
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  7. Mikosza, A. S. J., T. La, W. B. de Boer, and D. J. Hampson. 2001. The comparative prevalence of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli as the etiologic agents of histologically identified intestinal spirochetosis in Australia. J. Clin. Microbiol. 39:347-350.[Abstract/Free Full Text]
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  8. Mikosza, A. S. J., T. La, K. R. Margawani, C. J. Brooke, and D. J. Hampson. 2001. PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces. FEMS Microbiol. Lett. 197:167-170.[CrossRef][Medline]
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  9. Munshi, M. A., N. M. Taylor, A. S. J. Mikosza, P. B. S. Spencer, and D. J. Hampson. 2003. PCR detection and isolation of the anaerobic intestinal spirochete Brachyspira aalborgi from the feces of captive nonhuman primates. J. Clin. Microbiol. 41:1187-1191.[Abstract/Free Full Text]
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  10. Trivett-Moore, N. L., G. L. Gilbert, C. L. H. Law, D. J. Trott, and D. J. Hampson. 1998. Isolation of Serpulina pilosicoli from rectal biopsy specimens showing evidence of intestinal spirochetosis. J. Clin. Microbiol. 36:261-265.[Abstract/Free Full Text]
Andrew S. J. Mikosza
David J. Hampson*
School of Veterinary and Biomedical Sciences
Murdoch University
Murdoch, Western Australia 6150, Australia

Marion P.G. Koopmans
Yvonne T. H. P. van Duynhoven
National Institute of Public Health  and the Environment
Bilthoven, The Netherlands

* Phone: 61 08 9360 2287
Fax: 61 08 9310 4144
E-mail: D. Hampson{at}murdoch.edu.au

Journal of Clinical Microbiology, September 2003, p. 4492, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4492.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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