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Journal of Clinical Microbiology, March 2004, p. 1360-1362, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1360-1362.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development of a Diagnostic PCR Assay Targeting the Mn-Dependent Superoxide Dismutase Gene (sodA) for Identification of Streptococcus gallolyticus

Eiki Sasaki,1 Ro Osawa,1* Yosuke Nishitani,1 and Robert A. Whiley2

Department of Bioscience, Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe 657-8501, Japan,1 Department of Clinical and Diagnostic Oral Sciences, Barts and The London, Queen Mary's School of Medicine and Dentistry, London E1 2AD, United Kingdom2

Received 12 August 2003/ Returned for modification 10 November 2003/ Accepted 3 December 2003


   ABSTRACT
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 Abstract
Introduction
 References
 
A PCR-based assay to identify Streptococcus gallolyticus has been developed. The assay uses an oligonucleotide primer pair targeting a partial sequence of the manganese-dependent superoxide dismutase gene (sodA). The assay distinguished members of the S. gallolyticus group from other, closely related taxa successfully by yielding a 408-bp specific amplicon.


   INTRODUCTION
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 Abstract
 Introduction
References
 
Streptococcus gallolyticus is often found as a normal member of the gut microflora of various animals (15), while it has been reported to cause mastitis in cattle (8), septicemia in pigeons (3), and meningitis, sepsis, and endocarditis in humans (2, 4, 12). There have also been a number of case reports that suggest an etiological link between underlying infection with this organism and colon cancers in human (1, 5, 9, 10, 11). Most, if not all, of the strains belonging to this species are able to decarboxylate gallate as well as produce tannase, by which a hydrolyzable tannin (i.e., gallotannin) is hydrolyzed to release gallic acid, which is subsequently decarboxylated to pyrogallol (14). The species, which includes strains formerly identified as S. bovis biotypes I and II/2, can be distinguished from other, related taxa or biotypes (i.e., S. equinus, S. bovis biotype II/1) on the basis of the results of DNA-DNA reassociation experiments (14). In a recent taxonomic review, Facklam (6) has suggested that all human isolates of S. bovis biotypes I and II/2 are officially identified as S. gallolyticus. Nevertheless, the use of the name S. bovis biotype I or II/2 instead of S. gallolyticus has been a common occurrence in clinical microbiology despite the clearly established status of the latter name (14). This situation seems to have come about in order to avoid confusion among clinical microbiologists. Recently, Poyart et al. (18) demonstrated that a partial sequence of the manganese-dependent superoxide dismutase gene (sodA) provides useful information for the differentiation of species of the so-called S. bovis-S. equinus group. Here we evaluated the use of a PCR-based method for identification of S. gallolyticus that uses a primer pair targeting sodA by using strains of the S. bovis-S. equinus group from our culture collection with known DNA-DNA homology status.

Twenty-three streptococcal strains that had been previously designated S. gallolyticus (14) and seven strains that had been assigned by Farrow et al. (7) to S. equinus were used in the present study. Whole genomic DNA-DNA homology had been used previously (14) to determine the taxonomic position of these strains, in which the relative percent DNA binding of each strain with S. gallolyticus ACM 3611T was measured (Table 1). The S. gallolyticus strains consisted of 12 fecal or rumen isolates from various animals and 11 clinical isolates from cases of cow mastitis and human septicemia or endocarditis. It should be noted that the seven S. equinus strains included five strains originally received as S. bovis. All strains were cultured at 37°C on Columbia blood agar (Oxoid Ltd., Basingstoke, United Kingdom) with 5% defibrinated horse blood prior to use. The strains were characterized for their biochemical properties with a commercial identification kit (API 20 Strep; bioMérieux, Lyon, France). Tannase and gallic acid decarboxylating activities of strains were determined by visual methods described elsewhere (16, 17).


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  		TABLE 1. Strains used in this study and their biochemical and genotypic characteristics

 
The sodA gene sequences of S. gallolyticus CIP105428T (GenBank accession no. AJ297183), S. bovis CIP102302 (GenBank accession no. Z95896), and S. equinus 102504T (GenBank accession no. Z95903) from the GenBank database were aligned and scanned for variable regions that could provide a suitable primer set specific to S. gallolyticus (Fig. 1). On the basis of this analysis, forward primer SgsodA-F (5'-CAATGACAATTCACCATGA-3'; positions 17 to 34) and reverse primer SgsodA-R (5'-TTGGTGCTTTTCCTTGTG-3'; positions 424 to 407) were designed as shown in Fig. 1. With this primer set, PCR amplification was performed in a total volume of 20 µl. Whole genomic DNA from each isolate was prepared by the method of Marmur (13). Two microliters of each genomic DNA preparation (10 ng of DNA/µl of Tris-EDTA) was added to the PCR master mixture, which consisted of 2 µl of 10x PCR buffer (Mg2+ free; Promega Corporation, Madison, Wis.), 2 µl of 25 mM MgCl2, 0.4 µl of a 10 mM deoxynucleoside triphosphate mixture, 0.5 µl of each primer (10 pmol/µl), and 0.1 µl (0.5 U) of Taq DNA polymerase (Promega), with the remaining volume consisting of distilled water. A GeneAmp PCR System 2700 thermal cycler (Applied Biosystems, Foster City, Calif.) was used for PCR amplification consisting of an initial denaturation at 94°C for 5 min; 30 cycles of denaturation at 94°C for 60 s, annealing at 54°C for 30 s, and extension at 72°C for 30 s; and a final extension at 72°C for 7 min. Five microliters of the PCR products was electrophoresed on 2% agarose gels, stained with ethidium bromide (0.25 µg/ml), and photographed under UV light.



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  		FIG. 1. Alignments of the sodA gene DNA sequences used in the present study and retrieved from the GenBank database (S. gallolyticus CIP105428T [GenBank accession no. AJ297183], S. bovis CIP102302 [GenBank accession no. Z95896], and S. equinus 102504T [GenBank accession no. Z95903]). Boldface letters indicate the positions of the primers in the sequences. The hyphens indicate the same bases as shown in S. gallolyticus CIP102302.

 
The PCR yielded an amplicon of 408 bp from all 23 S. gallolyticus strains, while other S. bovis-equinus strains did not (Table 1), suggesting that the assay is a useful molecular tool for the identification of S. gallolyticus. Further work with more strains of S. gallolyticus and other, related species is in progress in order to confirm the reliability of the PCR-based method as a diagnostic tool. With recently accumulating evidence of its taxonomic distinction and its etiological significance, we propose that the name S. gallolyticus be adopted for use by clinical microbiologists.


   FOOTNOTES
 
* Corresponding author. Mailing address: Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe City, Japan 657-8501. Phone and fax: 81-78-803-5804. E-mail: osawa{at}ans.kobe-u.ac.jp. Back


   REFERENCES
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 Abstract
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Journal of Clinical Microbiology, March 2004, p. 1360-1362, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1360-1362.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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