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Journal of Clinical Microbiology, December 2005, p. 6150-6151, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6150-6151.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Validation of Performance of Plastic versus Glass Bottles for Culturing Anaerobes from Blood in BacT/ALERT SN Medium

Clinical Microbiology Laboratory, Duke University Medical Center,¹ Departments of Pathology,² Medicine, Duke University School of Medicine, Durham, North Carolina 27710³

Received 26 September 2005/ Accepted 27 September 2005

	ABSTRACT

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To validate performance, we compared the new plastic BacT/ALERT (bioMérieux, Durham, NC) SN bottle to the current glass SN bottle with samples of blood obtained for culture from adults and found them comparable for both recovery and speed of detection of microorganisms. We conclude that the safety advantage of plastic bottles can be achieved without compromising performance.

	TEXT

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Both aerobic and anaerobic blood culture media are commonly used to detect microorganisms from the blood of adult patients with suspected bloodstream infection. bioMérieux, Inc. (Durham, NC) has reformulated its anaerobic standard culture medium in a clear plastic bottle for use in the BacT/ALERT blood culture instrument. The design of this plastic bottle is similar to that described by Snyder et al. for an aerobic standard SA medium (6) and Petti et al. for the adult aerobic FA (4) and pediatric PF (5) media. These plastic bottles with a repertoire of media were designed to eliminate breakage of glass bottles and thereby to minimize potential safety hazards.

The new plastic SN (PSN) bottle contains a casein-soy-based medium similar to that in glass SN (GSN) except for formula modifications that include addition of yeast extract, pyridoxine HCl, and sodium pyruvate. The bottle has a smaller stopper and contains a modified liquid emulsion sensor to reduce the risk of false-positive bottles. To assess the result of these changes, we compared the new PSN bottle to the existing GSN bottle when used in conjunction with an aerobic SA bottle for both the recovery of microorganisms and the time to detection of the growth in samples of blood obtained from adults with suspected bloodstream infection.

(This work was presented at the 103rd General Meeting of the American Society for Microbiology, Washington, DC, 19 May 2003, abstract C-003.)

Blood was collected from adults with suspected bloodstream infection who presented to Duke University Medical Center from April through December 2002. Institutional review board approval was obtained prior to the study, and all blood cultures were performed as part of standard patient care. Thirty milliliters of blood was obtained by venipuncture, and 10-ml aliquots were distributed into aerobic SA and anaerobic PSN and GSN bottles. Upon receipt in the laboratory, each bottle was visually compared to bottles of the same composition and medium marked at 8- and 12-ml added volumes in accord with laboratory protocols (4, 5). Only bottles containing 8 to 12 ml of blood were included in the analysis. Gram stains were done only on bottles flagged positive by the instrument, and these bottles were subcultured to appropriate media for subsequent identification of microorganisms by standard laboratory methods (3). Gram stain-negative bottles were returned to the instrument for the remainder of the 5-day incubation period or until they were reflagged by the instrument. False positives were defined as bottles that were Gram stain and subculture negative after the instrument signaled positive.

An infectious disease physician reviewed each positive culture and coded it as a true positive, a contaminant, or an isolate of unknown clinical importance based on previously published criteria (7). Episodes of bloodstream infection were defined by growth of a clinically important blood culture isolate without recovery of a different microorganism during the subsequent 7-day period. If a different clinically important microorganism was recovered within 72 h, the two isolates were considered a polymicrobial episode and were not included in the analysis. If a different clinically significant microorganism was recovered after 72 h, the second isolate was considered a new episode. Statistical analysis of results was performed with the modified chi-square test described by McNemar (1).

Of 5,323 anaerobic bottles received, 3,652 (69%) met the adequacy criteria, which is comparable to previous reports (4, 5). There were 357 isolates positive in one or both adequately filled bottles. Of these 357 isolates, 240 clinically significant microorganisms were isolated from 205 patients. Of the 240 isolates, 30 were fungi and 14 were aerobic gram-negative rods (e.g., Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia) that one would expect to isolate preferentially from an aerobic bottle (2). Therefore, we omitted these groups of microorganisms from further consideration to emphasize the relative performances of PSN and GSN for anaerobic and facultative bacteria. Overall, clinically significant isolates, including strict anaerobes, were detected with equal frequencies in both study bottles (Table 1). The relative paucity of anaerobes is in accord with trends of recent decades at similar centers (2). There were 136 isolates recovered from both PSN and GSN bottles within 5 days, and the mean times to detection (Table 2) were similar in both bottles (for PSN, 16.4 h; for GSN, 17.9 h). Among the 3,652 adequate paired blood culture bottles, there were 27 false-positive bottles in PSN (0.7%) and 30 in GSN (0.8%). Contaminants were isolated with equal frequencies from PSN bottles and GSN bottles (both, 20; PSN, 31; GSN, 21; P, not significant).

In this study, the overall performance of the anaerobic plastic bottle was similar to that of the glass bottle for both the recovery of clinically significant microorganisms and the time to detection of growth of anaerobic and facultative bacteria from adult patients with suspected bloodstream infection and is in accord with previously published reports with plastic PF medium for children and SA and FA media for adults (4-6). The plastic bottle is lighter than glass, resists breakage, and reduces the risk of exposure of healthcare personnel to infectious substances. Therefore, we recommend the use of PSN bottles in the BacT/ALERT blood culture system.

	ACKNOWLEDGMENTS

 
We thank the staff of the Clinical Microbiology Laboratory at Duke University Medical Center.

This work was supported by a grant from bioMérieux, Inc., Durham, NC.

	FOOTNOTES

 
* Corresponding author. Mailing address: Duke University Medical Center, Clinical Microbiology Laboratory, 116 CARL Bldg., Research Drive, Durham, NC 27710. Phone: (919) 684-2562. Fax: (919) 684-8519. E-mail: Stanley.Mirrett{at}duke.edu.

	REFERENCES

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1. McNemar, Q. 1962. Psychological statistics, p. 209-239. John Wiley & Sons, Inc., New York, N.Y.
2. Murray, P. R., P. Traynor, and D. Hopson. 1992. Critical assessment of blood culture techniques: analysis of recovery of obligate and facultative anaerobes, strict aerobic bacteria, and fungi in aerobic and anaerobic blood culture bottles. J. Clin. Microbiol. 30:1462-1468.[Abstract/Free Full Text]
3. Murray, P. R., E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken. 1999. Manual of clinical microbiology. ASM Press, Washington, D.C.
4. Petti, C. A., S. Mirrett, C. W. Woods, and L. B. Reller. 2005. Controlled clinical comparison of plastic and glass bottles of BacT/ALERT FA medium for culturing organisms from blood of adult patients. J. Clin. Microbiol. 43:1960-1962.[Abstract/Free Full Text]
5. Petti, C. A., S. Mirrett, C. W. Woods, and L. B. Reller. 2005. Controlled clinical comparison of plastic versus glass bottles of BacT/ALERT PF medium for culturing blood from children. J. Clin. Microbiol. 43:445-447.[Abstract/Free Full Text]
6. Snyder, J. W., G. K. Munier, G. D. Bostic, P. S. Bozigar, and R. Hanna. 2002. Evaluation of a plastic nonvented aerobic blood culture bottle for use with the BacT/ALERT microbial detection system. J. Clin. Microbiol. 40:4757-4759.[Abstract/Free Full Text]
7. Weinstein, M. P., M. L. Towns, S. M. Quartey, S. Mirrett, L. G. Reimer, G. Parmigiani, and L. B. Reller. 1997. The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults. Clin. Infect. Dis. 24:584-602.[Medline]

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