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Journal of Clinical Microbiology, February 2006, p. 619-621, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.619-621.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

High Interlaboratory Reproducibility of DNA Sequence-Based Typing of Bacteria in a Multicenter Study

M. Aires-de-Sousa,1 K. Boye,2 H. de Lencastre,1 A. Deplano,3 M. C. Enright,4 J. Etienne,5 A. Friedrich,6 D. Harmsen,7* A. Holmes,8 X. W. Huijsdens,9 A. M. Kearns,8 A. Mellmann,6 H. Meugnier,5 J. K. Rasheed,10 E. Spalburg,9 B. Strommenger,11 M. J. Struelens,3 F. C. Tenover,10 J. Thomas,4 U. Vogel,12 H. Westh,2 J. Xu,13 and W. Witte11

Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal,1 Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark,2 Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium,3 Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom,4 Laboratoire Central de Microbiologie, Hôpital Edouard Herriot, Lyon, France,5 Institut für Hygiene, University Hospital Münster, Münster, Germany,6 Department of Periodontology, University Hospital Münster, Münster, Germany,7 Center for Infections, Health Protection Agency, London, United Kingdom,8 Diagnostic Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands,9 Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia,10 Robert-Koch-Institut, Wernigerode, Germany,11 Institut für Hygiene und Microbiologie, University Hospital Würzburg, Würzburg, Germany,12 State Key Laboratory of Infectious Diseases Prevention and Control, National Institute for Communicable Disease Prevention and Control, Beijing, People's Republic of China,13

Received 29 June 2005/ Returned for modification 2 September 2005/ Accepted 28 November 2005


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ABSTRACT
 
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.


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TEXT
 
Typing of pathogens is essential to detect outbreaks and to forecast future trends. For Staphylococcus aureus, a major healthcare-associated pathogen worldwide, pulsed-field gel electrophoresis (PFGE) is currently regarded as the molecular typing "gold standard" (11). Significant efforts have been made in the past to harmonize protocols of PFGE and to establish a standardized nomenclature. However, these projects proved only partially successful when judged by reproducibility, speed, and costs of analysis (11, 16, 17). To improve the speed of typing, DNA sequence-based approaches, such as multilocus sequence typing (MLST), are coming to be more frequently used for S. aureus (4). However, MLST is not suitable for routine surveillance of methicillin-resistant S. aureus (MRSA) because of higher costs and lower discriminatory power compared to PFGE. Frenay et al. developed a single-locus sequence typing method for S. aureus using the sequences of polymorphic region X of the S. aureus protein A (spa) gene (5). Subsequently, it was shown by calculation of Simpson's index of diversity that spa typing is nearly as discriminatory as PFGE (7, 13). To date, no study has investigated the interlaboratory comparability of DNA sequence-based genetic typing methods. Therefore, we evaluated the reproducibility of spa sequencing in a multicenter study using specialized software for sequence analysis.

(This study was presented in part at the 105th General Meeting of the American Society for Microbiology in Atlanta, GA, 5 to 9 June 2005.)

To examine interlaboratory comparability, 30 blind-coded S. aureus DNA samples (Table 1) were shipped worldwide by mail to 11 laboratories with personnel experienced in DNA sequencing (Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal; Hvidovre Hospital, Hvidovre, Denmark; Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium; Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom; Laboratoire Central de Microbiologie, Hôpital Edouard Herriot, Lyon, France; Institut für Hygiene, University Hospital Münster, Münster, Germany; Center for Infections, Health Protection Agency, London, United Kingdom; Diagnostic Laboratory of Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands; Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, GA; Institut für Hygiene und Microbiologie, University Hospital Würzburg, Würzburg, Germany; State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Prevention and Control, Beijing, People's Republic of China). Isolates 2, 3, 9, 12, and 28, representing different clonal lineages of MRSA, had been included in an international study on the evolutionary origin of MRSA (3). The remaining isolates used in this study originated from the strain collection of the German Reference Center for Staphylococci and were unrelated with respect to time and place of infection. Of the 30 DNA samples, one (no. 2) was supplied in four replicates (11, 15, 17, and 25) to test intralaboratory reproducibility (Table 1). Investigators sequenced the spa gene by using in-house sequencing platforms and analyzed the resulting forward and reverse sequence chromatograms (ABI, SCF, or FASTA file format) with the Ridom StaphType software (Ridom GmbH, Würzburg, Germany). The software attaches to each called base a quality value that corresponds to a sequence error probability. Taking the quality values into consideration, the software constructs a consensus sequence, automatically detects the spa repeats, and assigns a spa type. In at least 95% of all cases, no further manual editing is necessary. For the remaining sequences, a graphic user interface allows the user to edit sequences manually by means of an integrated expert system (6). A spa type is deduced from the order of specific repeats. Exchange of a single base results in a different spa type.


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  		TABLE 1. Strains used for interlaboratory comparison of spa typing

A spa PCR and sequencing protocol was made available. The protocol suggested using PuRe Taq Ready-To-Go PCR Beads (Amersham Biosciences, Freiburg, Germany) in a total volume of 25 µl containing LiChrosolv water (Merck, Darmstadt, Germany), DNA, and 10 pmol each of high-performance liquid chromatography-cleaned primers spa-1113f (5'-TAA AGA CGA TCC TTC GGT GAG C-3') and spa-1514r (5'-CAG CAG TAG TGC CGT TTG CTT-3'). Thermal cycling reaction conditions consisted of initial denaturation (5 min at 80°C), followed by 35 cycles of denaturation (45 s at 94°C), annealing (45 s at 60°C), and extension (90 s at 72°C), and finishing with a single extension (10 min at 72°C). Twenty microliters of each PCR product was purified with the QIAquick PCR Purification Kit (QIAGEN, Hilden, Germany) and sequenced with the ABI Prism BigDye Terminator v3.1 Ready Reaction Cycle Sequencing Kit (Applied Biosystems, Darmstadt, Germany). The sequencing reaction mixture contained LiChrosolv, 0.5 µl of premix, and 2 µl of ABI 5x Sequencing Buffer from the kit; 10 pmol of sequencing primer; and 2 µl of cleaned PCR product in a total volume of 10 µl. The amplification primers were used for sequencing by 25 cycles of denaturation (10 s at 96°C) and extension (4 min at 60°C) at a thermal ramping rate of 1°C/s. The sequencing products were purified with a DyeEx 2.0 Spin Kit (QIAGEN) and then prepared for running on an ABI 3100 Avant Genetic Analyzer in accordance with the manufacturer's (Applied Biosystems) instructions. Apart from the primers, all participants in this study were free to use their routine procedures.

One laboratory did not receive the DNA samples; the remaining 10 laboratories were able to determine and analyze the sequences of the 30 DNA samples with the Ridom StaphType software. Harmonization and adherence to the recommended protocol, which are typically requirements in most typing studies (11, 15, 17), were not needed. Each laboratory determined 8,691 bp (range, 110 to 422 bp per strain), and all participants reported exactly the same spa type for each of the isolates analyzed. Therefore, the intra- and interlaboratory reproducibility of the sequencing results was 100% each. Sending DNA samples instead of bacterial isolates facilitated the exchange of specimens. Our results prove the excellent reproducibility of sequence-based typing methods due to the unambiguous nature of sequence data. In addition, the use of the StaphType software ensured a uniform terminology by synchronization with an accompanying public website administering all new spa repeat and type codes (6). Only high-quality sequence data are automatically accepted by the server (http://www.SpaServer.ridom.de), and therefore no curator is needed for administration of the database or analyses of newly submitted sequences and strains, in contrast to other typing networks, e.g., MLST.net (http://www.mlst.net).

This study presents a global multicenter evaluation of DNA sequence-based typing in bacteriology. Due to its clinical importance and the availability of specialized software, S. aureus was chosen as the model organism for sequence-based typing. Numerous collaborative studies have shown limited intra- and interlaboratory reproducibility for PFGE (1, 10, 11, 17). Other molecular typing methods, e.g., repetitive-element PCR, also failed to achieve satisfying reproducibility (2). Only the reproducibility of DNA sequencing was the subject of this study, whereas the typeability, discriminatory power, and stability of spa sequences (14) have already been demonstrated elsewhere (5, 6, 8). As costs associated with DNA sequencing are decreasing, this methodology now is a viable alternative for molecular typing of pathogens and becomes feasible even for smaller laboratories (12). Its demonstrated portability and reproducibility offer the prospect of a future "molecular Esperanto" typing standard (9).

In conclusion, genotyping of bacterial isolates by DNA sequencing proved to be a highly reproducible and robust technique even without extensive harmonization of laboratory protocols. The use of specialized software for the analysis of sequencing data ensured a common typing nomenclature and thus greatly facilitated the exchange of typing data.


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FOOTNOTES
 
* Corresponding author. Mailing address: Department of Periodontology, University Hospital Münster, Waldeyerstrasse 30, D-48149 Münster, Germany. Phone: 49-251-9802875. Fax: 49-251-9802868. E-mail: dharmsen{at}uni-muenster.de. Back


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Journal of Clinical Microbiology, February 2006, p. 619-621, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.619-621.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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