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Journal of Clinical Microbiology, August 2006, p. 2994-2996, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00846-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Multiple Cases of Familial Transmission of Community-Acquired Methicillin-Resistant Staphylococcus aureus

X. W. Huijsdens,* M. G. van Santen-Verheuvel, E. Spalburg, M. E. O. C. Heck, G. N. Pluister, B. A. Eijkelkamp, A. J. de Neeling, and W. J. B. Wannet

National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands

Received 21 April 2006/ Accepted 4 June 2006


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ABSTRACT
 
The worldwide emergence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) can have severe public health implications. Familial transmissions of CA-MRSA in The Netherlands were investigated. Among the families studied, two clusters of CA-MRSA could be identified. This report demonstrates that family members can serve as reservoirs of CA-MRSA which may become a serious problem in containing the spread of MRSA.


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TEXT
 
Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen causing nosocomial infections worldwide. MRSA strains have largely been confined to hospitals and long-term care facilities but are also emerging in the community (22). The differentiation between community-acquired MRSA (CA-MRSA) and hospital-associated MRSA is becoming difficult since CA-MRSA could spread into hospitals (25). CA-MRSA causes primary skin infections, mainly furunculosis and abscesses, but can also cause necrotizing tissue infections and fulminant pneumonia in young and previously healthy individuals (4, 11, 18, 23). Most CA-MRSA isolates contain the virulence factor Panton-Valentine leucocidin (PVL) and carry staphylococcal cassette chromosome mec (SCCmec) type IV or V. The combination of the PVL loci, the mecA gene, and the spread of CA-MRSA makes this MRSA a well-adapted pathogen. It is of interest to see to what extent PVL-MRSA transmission within families occurs, especially in families with young children. Transmission of MRSA between patients receiving health care and family members (1, 6, 24) and case reports of intrafamilial spread of CA-MRSA have been described (3, 9, 16, 19). However, typing data from these familial MRSA transmissions is limited or even unknown.

The aim of this study was to determine the intrafamilial transmission of CA-MRSA in The Netherlands during a 2-year period (2003 and 2004). Approximately 10% of all Dutch MRSA isolates carry the genes for PVL (25). The MRSA strains associated with familial transmission were characterized by pulsed-field gel electrophoresis (PFGE) (13), protein A (spa) typing (5), multilocus sequence typing (2), SCCmec multiplex PCR (7, 15), and accessory gene regulator (AGR) typing (10). A toxin profile PCR was also determined (12). For the detection of exfoliative toxin D, oligonucleotides were developed on the basis of the nucleotide sequence deposited in the GenBank database under accession no. AB057421. The sequences of the exfoliative toxin D forward and reverse primers were 5'-ACTATCATGTATCAAGGATGGC-3' and 5'-CAAATCAGTTCCTTGTCCAT-3', respectively. Both primers were used at a concentration of 0.8 µM. Family transmission was defined as two or more members within a family, living at the same postal address, who were colonized with an MRSA strain having the same PFGE type. In 2003 and 2004, 10 PVL-MRSA familial transmissions were found. In 7 out of the 10 families, skin infections were reported, and in 6 families a link with a foreign country was noted. The transmissions involved at least two family members, up to four members within a family of six. A total of 27 MRSA isolates were studied. The results (Table 1) showed that all MRSA strains within a family had the same typing characteristics, indicating the transmission of CA-MRSA within families. In 7 of the 10 families, it involved transmission from parent to child or vice versa. In two cases of parent-child transmission, it involved two affected siblings (families 3 and 6), and in one case three siblings were affected (family 10). In two cases (families 6 and 10), some family members were colonized with a PVL-negative MRSA (data not shown). Interestingly, in family 10 the PVL-negative MRSA of the mother (age 36) and one sibling (male, age 3) had the same PFGE type as the PVL-positive strains of the father and three siblings. No further typing of the PVL-negative strains was performed. Presumably, the MRSA strains of the father and siblings obtained PVL bacteriophages (14). This hypothesis was strengthened by the fact that the upper band in the PFGE pattern of the PVL-negative MRSA strains was about 41 kb smaller, approximately the size of a PVL phage genome.


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  		TABLE 1. Characteristics of family members colonized with PVL-positive MRSAa

Remarkably, twice the MRSA strains of two families (families 1 and 6 and families 4 and 8) had identical typing characteristics. MRSA strains with PFGE type 50A (according to the Dutch PFGE nomenclature classification) are rare in our national MRSA database. Further investigation revealed that families 1 and 6 lived in the same neighborhood. This indicates a possible spread of an MRSA strain not only within a family but also between families. Whether there is any relationship between the two families is unknown. Families 4 and 8 lived in different areas of The Netherlands.

Seven out of the 10 families could be grouped into two clusters: the well-known ST8 (USA300) clone and the ST59 CA-MRSA clone. Remarkably, all MRSA strains with ST8 were spa type t008, SCCmec type IV, AGR type 1, and LukED positive (families 2, 4, 5, and 8). The PFGE types of the ST8 MRSA strains were 46, 206, and 218. These PFGE types are closely related (≥80%), indicating a clonal relatedness. This PFGE cluster is identical to the well-known PFGE USA300 CA-MRSA clone, which has been found in several outbreaks in the United States. Our findings confirm the recently described transatlantic spread of the USA300 clone (20, 26). A second MRSA cluster was also detected; the MRSA strains of families 1, 3, and 6 seemed to be related to each other as well. The MRSA strains in this cluster were ST59, either spa type t437 or spa type t441 (two closely related spa types), SCCmec type V or nontypeable, AGR type 1, and SEB positive. MRSA ST59 is also a well-known CA-MRSA which has been found in different parts of the world (8, 17, 25). MRSA ST80 was only found in one family. The ST80-IV clone is a well-known CA-MRSA in Europe (25, 27) and was also found in familial transmissions in Denmark (21).

In conclusion, transmission of community-acquired MRSA outside the healthcare setting poses a threat to public health and may be a serious problem in containing the spread of MRSA. This report demonstrates that family members can serve as reservoirs of PVL-positive MRSA and that transmission can occur among family members. The prevalence of family transmission is probably much higher than that described in this study because family members of a patient are not routinely tested for MRSA colonization. In 7 of the 10 families, young children no more than 8 years old were involved. When a child gets pneumonia, possibly preceded by flu-like symptoms, it is important to consider MRSA infection especially when one of the family members is known to be MRSA positive.


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FOOTNOTES
 
* Corresponding author. Mailing address: National Institute for Public Health and the Environment (RIVM), Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Phone: (31) 30 2744117. Fax: (31) 30 2744418. E-mail: Xander.Huijsdens{at}rivm.nl. Back


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REFERENCES
 
    1
  1. Calfee, D. P., L. J. Durbin, T. P. Germanson, D. M. Toney, E. B. Smith, and B. M. Farr. 2003. Spread of methicillin-resistant Staphylococcus aureus (MRSA) among household contacts of individuals with nosocomially acquired MRSA. Infect. Control Hosp. Epidemiol. 24:422-426.[CrossRef][Medline]
    2. 2
  2. Enright, M. C., N. P. Day, C. E. Davies, S. J. Peacock, and B. G. Spratt. 2000. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38:1008-1015.[Abstract/Free Full Text]
    3. 3
  3. Faden, H., and S. Ferguson. 2001. Community-acquired methicillin-resistant Staphylococcus aureus and intrafamily spread of pustular disease. Pediatr. Infect. Dis. J. 20:554-555.[Medline]
    4. 4
  4. Gillet, Y., B. Issartel, P. Vanhems, J. C. Fournet, G. Lina, M. Bes, F. Vandenesch, Y. Piemont, N. Brousse, D. Floret, and J. Etienne. 2002. Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet 359:753-759.[CrossRef][Medline]
    5. 5
  5. Harmsen, D., H. Claus, W. Witte, J. Rothganger, H. Claus, D. Turnwald, and U. Vogel. 2003. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J. Clin. Microbiol. 41:5442-5448.[Abstract/Free Full Text]
    6. 6
  6. Hollis, R. J., J. L. Barr, B. N. Doebbeling, M. A. Pfaller, and R. P. Wenzel. 1995. Familial carriage of methicillin-resistant Staphylococcus aureus and subsequent infection in a premature neonate. Clin. Infect. Dis. 21:328-332.[Medline]
    7. 7
  7. Ito, T., X. X. Ma, F. Takeuchi, K. Okuma, H. Yuzawa, and K. Hiramatsu. 2004. Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC. Antimicrob. Agents Chemother. 48:2637-2651.[Abstract/Free Full Text]
    8. 8
  8. Ko, K. S., J. Y. Lee, J. Y. Suh, W. S. Oh, K. R. Peck, N. Y. Lee, and J. H. Song. 2005. Distribution of major genotypes among methicillin-resistant Staphylococcus aureus clones in Asian countries. J. Clin. Microbiol. 43:421-426.[Abstract/Free Full Text]
    9. 9
  9. L'Heriteau, F., J. C. Lucet, A. Scanvic, and E. Bouvet. 1999. Community-acquired methicillin-resistant Staphylococcus aureus and familial transmission. JAMA 282:1038-1039.[Free Full Text]
    10. 10
  10. Lina, G., F. Boutite, A. Tristan, M. Bes, J. Etienne, and F. Vandenesch. 2003. Bacterial competition for human nasal cavity colonization: role of staphylococcal agr alleles. Appl. Environ. Microbiol. 69:18-23.[Abstract/Free Full Text]
    11. 11
  11. Lina, G., Y. Piemont, F. Godail-Gamot, M. Bes, M. O. Peter, V. Gauduchon, F. Vandenesch, and J. Etienne. 1999. Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin. Infect. Dis. 29:1128-1132.[CrossRef][Medline]
    12. 12
  12. Mehrotra, M., G. Wang, and W. M. Johnson. 2000. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J. Clin. Microbiol. 38:1032-1035.[Abstract/Free Full Text]
    13. 13
  13. Murchan, S., M. E. Kaufmann, A. Deplano, R. de Ryck, M. Struelens, C. E. Zinn, V. Fussing, S. Salmenlinna, J. Vuopio-Varkila, N. El Solh, C. Cuny, W. Witte, P. T. Tassios, N. Legakis, W. van Leeuwen, A. van Belkum, A. Vindel, I. Laconcha, J. Garaizar, S. Haeggman, B. Olsson-Liljequist, U. Ransjo, G. Coombes, and B. Cookson. 2003. Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J. Clin. Microbiol. 41:1574-1585.[Abstract/Free Full Text]
    14. 14
  14. Narita, S., J. Kaneko, J. Chiba, Y. Piemont, S. Jarraud, J. Etienne, and Y. Kamio. 2001. Phage conversion of Panton-Valentine leukocidin in Staphylococcus aureus: molecular analysis of a PVL-converting phage, phiSLT. Gene 268:195-206.[CrossRef][Medline]
    15. 15
  15. Oliveira, D. C., and H. de Lencastre. 2002. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 46:2155-2161.[Abstract/Free Full Text]
    16. 16
  16. Osterlund, A., G. Kahlmeter, L. Bieber, A. Runehagen, and J. M. Breider. 2002. Intrafamilial spread of highly virulent Staphylococcus aureus strains carrying the gene for Panton-Valentine leukocidin. Scand. J. Infect. Dis. 34:763-764.[CrossRef][Medline]
    17. 17
  17. Pan, E. S., B. A. Diep, E. D. Charlebois, C. Auerswald, H. A. Carleton, G. F. Sensabaugh, and F. Perdreau-Remington. 2005. Population dynamics of nasal strains of methicillin-resistant Staphylococcus aureus—and their relation to community-associated disease activity. J. Infect. Dis. 192:811-818.[CrossRef][Medline]
    18. 18
  18. Reichert, B., G. Birrell, and G. Bignardi. 2005. Severe non-pneumonic necrotising infections in children caused by Panton-Valentine leukocidin producing Staphylococcus aureus strains. J. Infect. 50:438-442.[CrossRef][Medline]
    19. 19
  19. Simpson, A. J., J. R. Anderson, and G. A. Farfan. 1995. Family infection with methicillin-resistant Staphylococcus aureus. Lancet 346:914.[Medline]
    20. 20
  20. Tietz, A., R. Frei, and A. F. Widmer. 2005. Transatlantic spread of the USA300 clone of MRSA. N. Engl. J. Med. 353:532-533.[Free Full Text]
    21. 21
  21. Urth, T., G. Juul, R. Skov, and H. C. Schonheyder. 2005. Spread of a methicillin-resistant Staphylococcus aureus ST80-IV clone in a Danish community. Infect. Control Hosp. Epidemiol. 26:144-149.[CrossRef][Medline]
    22. 22
  22. Vandenesch, F., T. Naimi, M. C. Enright, G. Lina, G. R. Nimmo, H. Heffernan, N. Liassine, M. Bes, T. Greenland, M. E. Reverdy, and J. Etienne. 2003. Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwide emergence. Emerg. Infect. Dis. 9:978-984.[Medline]
    23. 23
  23. van der Flier, M., N. B. van Dijk, A. C. Fluit, A. Fleer, T. F. Wolfs, and J. P. van Gestel. 2003. Fatal pneumonia in an adolescent due to community-acquired methicillin-resistant Staphylococcus aureus positive for Panton-Valentine-leukocidin. Ned. Tijdschr. Geneeskd. 147:1076-1079.[Medline]
    24. 24
  24. Wagenvoort, J. H., E. I. De Brauwer, M. L. Sijstermans, and H. M. Toenbreker. 2005. Risk of re-introduction of methicillin-resistant Staphylococcus aureus into the hospital by intrafamilial spread from and to healthcare workers. J. Hosp. Infect. 59:67-68.[CrossRef][Medline]
    25. 25
  25. Wannet, W., M. Heck, G. Pluister, E. Spalburg, M. Van Santen, X. Huijsdens, E. Tiemersma, and A. J. De Neeling. 2004. Panton-Valentine leukocidin positive MRSA in 2003: the Dutch situation. Eurosurveillance 9:28-29.
    26. 26
  26. Wannet, W. J., E. Spalburg, M. E. Heck, G. N. Pluister, E. Tiemersma, R. J. Willems, X. W. Huijsdens, A. J. De Neeling, and J. Etienne. 2005. Emergence of virulent methicillin-resistant Staphylococcus aureus strains carrying Panton-Valentine leucocidin genes in The Netherlands. J. Clin. Microbiol. 43:3341-3345.[Abstract/Free Full Text]
    27. 27
  27. Witte, W., C. Braulke, C. Cuny, B. Strommenger, G. Werner, D. Heuck, U. Jappe, C. Wendt, H. J. Linde, and D. Harmsen. 2005. Emergence of methicillin-resistant Staphylococcus aureus with Panton-Valentine leukocidin genes in central Europe. Eur. J. Clin. Microbiol. Infect. Dis. 24:1-5.[CrossRef][Medline]

Journal of Clinical Microbiology, August 2006, p. 2994-2996, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00846-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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