Files in this Data Supplement:

• Supplemental file 1 - Supplement to Materials and Methods (DNA isolation methods and procedures provided in detail), Table S1 (Listing of the B. anthracis strains selected for sequencing to confirm the genotypes at the different SNP loci), Table S2 (Average cycle threshold values for the six assays used on a 10-fold dilution of DNA template from the Ames and Vollum strains of B. anthracis), Table S3 (Amplification frequencies for assays at 10 fg input template DNA for the Vollum and Ames strains of B. anthracis, along with a discussion of these low-level real-time PCRs in light of stochastic sampling problems encountered), and Fig. S1 (Phylogenetic relationships of the Ames strain and its close and distant B. anthracis strain relatives)
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