Quadruplex Real-Time PCR Assay Using Allele-Specific Scorpion Primers for Detection of Mutations Conferring Clarithromycin Resistance to Helicobacter pylori
J. Clin. Microbiol. Burucoa et al.
46: 2320
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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Legends of the supplemental figures.
MS Word document, 21K.
- Supplemental file 2
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Fig. S3, showing an example of a sensitivity assay performed on the SmartCycler thermocycler with 10-fold serial dilution of the genomic DNA purified from strain HP222 (A2143G).
Zipped TIF file, 1.9MB.
- Supplemental file 3
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Fig. S4, showing an example of a sensitivity assay performed on the SmartCycler thermocycler with 10-fold serial dilution of the genomic DNA purified from strain HP225 (A2142G).
Zipped TIF file, 1.9MB.
- Supplemental file 4
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Fig. S5, showing an example of a sensitivity assay performed on the SmartCycler thermocycler with 10-fold serial dilution of the genomic DNA purified from strain HP825 (A2142C).
Zipped TIF file, 1.9MB.
- Supplemental file 5
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Fig. S6, showing fluorescence curves obtained with mixtures of the Cla-resistant HP825 strain harboring the A2142C mutation and the wild-type Cla-susceptible J99 strain at different proportions.
Zipped TIF file, 938K.
