This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Richmond, S J Articles by Caul, E O Search for Related Content PubMed PubMed Citation Articles by Richmond, S J Articles by Caul, E O

 Previous Article  |  Next Article 

J Clin Microbiol. 1975 April; 1(4): 345-352

Fluorescent antibody studies in chlamydial infections.

ABSTRACT

Irradiated McCoy cells infected with genital strains of Chlamydia trachomatis were grown in wells on slides coated with polytetrafluoroethylene. The inclusions produced in this system formed the antigen in an indirect immunofluorescence test, which detected group-specific chlamydial antibodies in sera from patients attending veneral disease clinics. Chlamydial antibodies were found more frequently and in higher titer in sera from women attending veneral disease clinics then in sera from a less promiscuous population attending a Family Planning Association clinic. Paired sera from 13 patients with nongonococcal urethritis from whom chylamydiae had been isolated were tested against the homologous isolates; seroconversion was demonstrated in only one instance, and antibody was present in the first serum specimens of all the other patients. Chlamydia-specific immunoglobulin M was found in four of eight patients with psittacosis and in a proportion of sera from patients attending veneral disease and Family Planning Association clinics. The antigen for this immunofluorescence test can easily be prepared in laboratories with cell culture facilities for the isolation of C. trachomatis, and the test should be useful for laboratories which cannot undertake the micro-immunofluorescence test.

This article has been cited by other articles:

• Herring, A, Richens, J (2006). Lymphogranuloma venereum. Sex. Transm. Infect. 82: iv23-iv25 [Full Text]  
• Akande, V. A., Hunt, L. P., Cahill, D. J., Caul, E.O., Ford, W.C. L., Jenkins, J. M. (2003). Tubal damage in infertile women: prediction using chlamydia serology. Hum Reprod 18: 1841-1847 [Abstract] [Full Text]  
• Jones, C S, Maple, P A C, Andrews, N J, Paul, I D, Caul, E O (2003). Measurement of IgG antibodies to Chlamydia trachomatis by commercial enzyme immunoassays and immunofluorescence in sera from pregnant women and patients with infertility, pelvic inflammatory disease, ectopic pregnancy, and laboratory diagnosed Chlamydia psittaci/Chlamydia pneumoniae infection. J. Clin. Pathol. 56: 225-229 [Abstract] [Full Text]  
• Tapia, O., Slepenkin, A., Sevrioukov, E., Hamor, K., de la Maza, L. M., Peterson, E. M. (2002). Inclusion Fluorescent-Antibody Test as a Screening Assay for Detection of Antibodies to Chlamydia pneumoniae. CVI 9: 562-567 [Abstract] [Full Text]  
• Eley, A, Hemeg, H A, Geary, I, Ramsewak, S S, Herring, A, Caul, E O (2001). Prevalence of Chlamydia trachomatis IgG antibodies in antenatal patients from Trinidad. Sex. Transm. Infect. 77 : 301-302 [Full Text]