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J Clin Microbiol. 1992 August; 30(8): 2153-2157

Enterohemorrhagic Escherichia coli associated with hemolytic-uremic syndrome in Chilean children.

A Cordovéz, V Prado, L Maggi, J Cordero, J Martinez, A Misraji, R Rios, G Soza, A Ojeda and M M Levine

Microbiology Unit, Faculty of Medicine, University of Chile, Santiago.

ABSTRACT

A clinicoepidemiological study was undertaken to determine if enterohemorrhagic Escherichia coli (EHEC) was associated with hemolytic-uremic syndrome (HUS) in children in Santiago, Valdivia, and Temuco, Chile. Prospective surveillance detected 20 hospitalized cases of HUS in children less than 4 years of age in these cities from March 1988 to March 1989. Each HUS patient was matched (by sex and age) with two control children (hospitalized elective-surgery patients). To detect EHEC, DNA from stool culture isolates of E. coli was detected by hybridization with biotin-labelled DNA probes specific for the EHEC virulence plasmid, Shiga-like toxin I (SLT-I) or SLT-II. Stool cultures from 6 of 20 cases (30%) and from 2 of 38 controls (5.3%) yielded EHEC (P = 0.0158). EHEC isolates from all HUS cases hybridized with the EHEC plasmid probe and with probes for SLT-I or -II (or both). The serogroups of the isolates included O157, O26, and O111. EHEC causes HUS in Chile, and the biotinylated gene probes are practical diagnostic tools for epidemiologic studies.

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J Clin Microbiol. 1992 August; 30(8): 2153-2157

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