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J Clin Microbiol. 1994 November; 32(11): 2858-2860

Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers.

P N Nyambi, K Fransen, H De Beenhouwer, E N Chomba, M Temmerman, J O Ndinya-Achola, P Piot and G van der Groen

Institute of Tropical Medicine, Antwerp, Belgium.

ABSTRACT

The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.

J Clin Microbiol. 1994 November; 32(11): 2858-2860




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