This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dylla, B. L.
Right arrow Articles by Cockerill, F. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dylla, B. L.
Right arrow Articles by Cockerill, F. R., 3rd

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Jan 1995, 222-224, Vol 33, No. 1
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Evaluation of an immunoassay for direct detection of Escherichia coli O157 in stool specimens

BL Dylla, EA Vetter, JG Hughes and FR Cockerill 3rd
Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.

An enzyme-linked immunosorbent assay (ELISA) produced by LMD Laboratories, Inc., Carlsbad, Calif., was compared with culture for the detection of Escherichia coli O157. Nine of 185 stool specimens evaluated had positive results by the LMD E. coli O157 ELISA and grew E. coli O157 on culture; 174 had negative by LMD E. coli O157 ELISA results and did not grow E. coli O157 on culture. Of 174 specimens negative by LMD E. coli O157 ELISA, 117 specimens grew other enteric pathogens: Campylobacter spp. (46 isolates), Salmonella spp. (43 isolates), Yersinia spp. (20 isolates), and Shigella spp. (8 isolates). There were two indeterminant results by the LMD E. coli O157 ELISA. One stool specimen did not grow other enteric pathogens on culture, and one grew a Campylobacter sp. on culture. Both had negative LMD E. coli O157 ELISA results upon repeat testing. The LMD E. coli O157 ELISA is an accurate, easy-to-read screening method for the detection of E. coli O157 in fecal specimens.


This article has been cited by other articles:

  • Kehl, S. C. (2002). Role of the Laboratory in the Diagnosis of Enterohemorrhagic Escherichia coli Infections. J. Clin. Microbiol. 40: 2711-2715 [Full Text]  
  • Holland, J. L., Louie, L., Simor, A. E., Louie, M. (2000). PCR Detection of Escherichia coli O157:H7 Directly from Stools: Evaluation of Commercial Extraction Methods for Purifying Fecal DNA. J. Clin. Microbiol. 38: 4108-4113 [Abstract] [Full Text]  
  • Kimura, R., Mandrell, R. E., Galland, J. C., Hyatt, D., Riley, L. W. (2000). Restriction-Site-Specific PCR as a Rapid Test To Detect Enterohemorrhagic Escherichia coli O157:H7 Strains in Environmental Samples. Appl. Environ. Microbiol. 66: 2513-2519 [Abstract] [Full Text]  
  • Linde, H.-J., Neubauer, H., Meyer, H., Aleksic, S., Lehn, N. (1999). Identification of Yersinia Species by the Vitek GNI Card. J. Clin. Microbiol. 37: 211-214 [Abstract] [Full Text]  
  • Louie, M., Read, S., Simor, A. E., Holland, J., Louie, L., Ziebell, K., Brunton, J., Hii, J. (1998). Application of Multiplex PCR for Detection of Non-O157 Verocytotoxin-Producing Escherichia coli in Bloody Stools: Identification of Serogroups O26 and O111. J. Clin. Microbiol. 36: 3375-3377 [Abstract] [Full Text]  
  • Nataro, J. P., Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»