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Journal of Clinical Microbiology, 01 1995, 239-241, Vol 33, No. 1
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Use of UV ParaLens adapter for detection of acid-fast organisms

KV Patterson, CL McDonald, BF Miller and KC Chapin
Department of Pathology, University of South Alabama, Mobile 36617.

Auramine-stained mycobacterial smears from 136 clinical specimens were interpreted by using the UV ParaLens adapter (Beckton Dickinson), and results were compared with smear interpretations using a traditional fluorescent microscope and culture. The sensitivity and specificity of the ParaLens were 84 and 93%, respectively. Smears yielding discrepant results were overstained by the Kinyoun method. Overall, the sensitivity of auramine-stained smears interpreted with the UV ParaLens was comparable to that of Kinyoun-stained smears.


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