Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR [published erratum appears in J Clin Microbiol 1995 May;33(5):1434]

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Journal of Clinical Microbiology, Jan 1995, 24-27, Vol 33, No. 1
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR [published erratum appears in J Clin Microbiol 1995 May;33(5):1434]

S Dutka-Malen, S Evers and P Courvalin
Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.

A PCR assay that allows simultaneous detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci (Enterococcus faecium, E. faecalis, E. gallinarum, and E. casseliflavus) was developed. This assay was based on specific amplification of internal fragments of genes encoding D- alanine:D-alanine ligases and related glycopeptide resistance proteins. The specificity of the assay was tested on 5 well-characterized glycopeptide-resistant strains and on 15 susceptible enterococcal type strains. Clinical isolates of enterococci that could not be identified to the species level by conventional methods were identified by the PCR test. This assay offers a specific and rapid alternative to antibiotic susceptibility tests, in particular for detection of low-level vancomycin resistance.

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Ligozzi, M., Bernini, C., Bonora, M. G., de Fatima, M., Zuliani, J., Fontana, R. (2002). Evaluation of the VITEK 2 System for Identification and Antimicrobial Susceptibility Testing of Medically Relevant Gram-Positive Cocci. J. Clin. Microbiol. 40: 1681-1686 [Abstract] [Full Text]
Vancanneyt, M., Lombardi, A., Andrighetto, C., Knijff, E., Torriani, S., Bjorkroth, K. J., Franz, C. M. A. P., Foulquie Moreno, M. R., Revets, H., De Vuyst, L., Swings, J., Kersters, K., Dellaglio, F., Holzapfel, W. H. (2002). Intraspecies Genomic Groups in Enterococcus faecium and Their Correlation with Origin and Pathogenicity. Appl. Environ. Microbiol. 68: 1381-1391 [Abstract] [Full Text]
Hancock, L. E., Gilmore, M. S. (2002). The capsular polysaccharide of Enterococcus faecalis and its relationship to other polysaccharides in the cell wall. Proc. Natl. Acad. Sci. USA 99: 1574-1579 [Abstract] [Full Text]
Lauderdale, T.-L., McDonald, L. C., Shiau, Y.-R., Chen, P.-C., Wang, H.-Y., Lai, J.-F., Ho, M. (2002). Vancomycin-Resistant Enterococci from Humans and Retail Chickens in Taiwan with Unique VanB Phenotype-vanA Genotype Incongruence. Antimicrob. Agents Chemother. 46: 525-527 [Abstract] [Full Text]
Novais, C., Vital, C., Ribeiro, G., Coque, T. M., Peixe, L. V. (2002). First characterization of vancomycin-resistant enterococci from a Portuguese hospital.. J Antimicrob Chemother 49: 215-217 [Full Text]
Antignac, A., Alonso, J.-M., Taha, M.-K. (2001). Nonculture Prediction of Neisseria meningitidis Susceptibility to Penicillin. Antimicrob. Agents Chemother. 45: 3625-3628 [Abstract] [Full Text]
Werner, G., Hildebrandt, B., Witte ;, W., Murray, B. E., Singh, K. V. (2001). The Newly Described msrC Gene Is Not Equally Distributed among All Isolates of Enterococcus faecium. Antimicrob. Agents Chemother. 45: 3672-3673 [Full Text]
Kawalec, M., Gniadkowski, M., Kedzierska, J., Skotnicki, A., Fiett, J., Hryniewicz, W. (2001). Selection of a Teicoplanin-Resistant Enterococcus faecium Mutant during an Outbreak Caused by Vancomycin-Resistant Enterococci with the VanB Phenotype. J. Clin. Microbiol. 39: 4274-4282 [Abstract] [Full Text]
Boerlin, P., Wissing, A., Aarestrup, F. M., Frey, J., Nicolet, J. (2001). Antimicrobial Growth Promoter Ban and Resistance to Macrolides and Vancomycin in Enterococci from Pigs. J. Clin. Microbiol. 39: 4193-4195 [Abstract] [Full Text]
Vergis, E. N., Hayden, M. K., Chow, J. W., Snydman, D. R., Zervos, M. J., Linden, P. K., Wagener, M. M., Schmitt, B., Muder, R. R. (2001). Determinants of Vancomycin Resistance and Mortality Rates in Enterococcal Bacteremia: A Prospective Multicenter Study. ANN INTERN MED 135: 484-492 [Abstract] [Full Text]
Fluit, A. C., Visser, M. R., Schmitz, F.-J. (2001). Molecular Detection of Antimicrobial Resistance. Clin. Microbiol. Rev. 14: 836-871 [Abstract] [Full Text]
Teng, L.-J., Hsueh, P.-R., Wang, Y.-H., Lin, H.-M., Luh, K.-T., Ho, S.-W. (2001). Determination of Enterococcus faecalis groESL Full-Length Sequence and Application for Species Identification. J. Clin. Microbiol. 39: 3326-3331 [Abstract] [Full Text]
Elsayed, S., Hamilton, N., Boyd, D., Mulvey, M., Kariyama, R., Mitsuhata, R., Kumon, H. (2001). Improved Primer Design for Multiplex PCR Analysis of Vancomycin-Resistant Enterococcus spp.. J. Clin. Microbiol. 39: 2367-2368 [Full Text]
Butaye, P., Devriese, L. A., Haesebrouck, F. (2001). Differences in Antibiotic Resistance Patterns of Enterococcus faecalis and Enterococcus faecium Strains Isolated from Farm and Pet Animals. Antimicrob. Agents Chemother. 45: 1374-1378 [Abstract] [Full Text]
Kirschner, C., Maquelin, K., Pina, P., Ngo Thi, N. A., Choo-Smith, L.-P., Sockalingum, G. D., Sandt, C., Ami, D., Orsini, F., Doglia, S. M., Allouch, P., Mainfait, M., Puppels, G. J., Naumann, D. (2001). Classification and Identification of Enterococci: a Comparative Phenotypic, Genotypic, and Vibrational Spectroscopic Study. J. Clin. Microbiol. 39: 1763-1770 [Abstract] [Full Text]

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