This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tiballi, R. N. Articles by Kauffman, C. A. Search for Related Content PubMed PubMed Citation Articles by Tiballi, R. N. Articles by Kauffman, C. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 10 1995, 2612-2615, Vol 33, No. 10
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Torulopsis glabrata: azole susceptibilities by microdilution colorimetric and macrodilution broth assays

RN Tiballi, LT Zarins, X He and CA Kauffman
Department of Internal Medicine, Veterans Affairs Medical Center, Ann Arbor, Michigan 48105, USA.

Fluconazole and itraconazole MICs were determined by both the standard macrodilution method of the National Committee for Clinical Laboratory Standards and a colorimetric broth microdilution method for 140 isolates of Torulopsis (Candida) glabrata obtained over a 15-year period. Using the method of the National Committee for Clinical Laboratory Standards the MICs at which 90% of isolates are inhibited (MIC50) for all isolates were 32 and 1.6 micrograms/ml for fluconazole and itraconazole, respectively. For fluconazole, the MIC90 rose from 16 to > 64 micrograms/ml when the MIC90s for isolates collected from July 1980 to June 1991 were compared with those for isolates collected from July 1991 to March 1995. For itraconazole, the MIC90s for isolates from the same time periods were 0.8 and 3.2 micrograms/ml, respectively. Although for isolates from some non-human immunodeficiency virus- infected patients the MICs rose, most of the high MICs were found for isolates from human immunodeficiency virus-infected patients who had been extensively treated with azole drugs for thrush. The colorimetric method yielded endpoints that were more definitive; concordances within 2 dilutions for the two methods were 87% for fluconazole and 86% for itraconazole.

This article has been cited by other articles:

• Kirkpatrick, W. R., Revankar, S. G., Mcatee, R. K., Lopez-Ribot, J. L., Fothergill, A. W., McCarthy, D. I., Sanche, S. E., Cantu, R. A., Rinaldi, M. G., Patterson, T. F. (1998). Detection of Candida dubliniensis in Oropharyngeal Samples from Human Immunodeficiency Virus-Infected Patients in North America by Primary CHROMagar Candida Screening and Susceptibility Testing of Isolates. J. Clin. Microbiol. 36: 3007-3012 [Abstract] [Full Text]