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Journal of Clinical Microbiology, 11 1995, 3061-3063, Vol 33, No. 11
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Application of PCR for detection of toxigenic Corynebacterium diphtheriae strains isolated during the Russian diphtheria epidemic, 1990 through 1994

VM Mikhailovich, VG Melnikov, IK Mazurova, IK Wachsmuth, JD Wenger, M Wharton, H Nakao and T Popovic
Russian Federal Reference Laboratory for Diphtheria, Gabrichevsky Institute of Epidemiology and Microbiology, Moscow, Russia.

A total of 250 Corynebacterium diphtheriae isolates from clinical cases and carriers in Russia were assayed by PCR directed at the A subunit of the diphtheria toxin gene to distinguish toxigenic from nontoxigenic strains; 170 strains were positive as indicated by the presence of the 248-bp amplicon. The results of this PCR assay were in complete concordance with those of the standard immunoprecipitation assay (Elek), and the PCR assay is a useful tool for rapid identification in clinical laboratories.


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