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Journal of Clinical Microbiology, 12 1995, 3191-3193, Vol 33, No. 12
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Evaluation of E-Test for determination of antimicrobial MICs for Pseudomonas aeruginosa isolates from cystic fibrosis patients

EF Marley, C Mohla and JM Campos
Department of Pathology, George Washington University Medical Center, Washington, DC 20037, USA.

We determined the E-Test and National Committee for Clinical Laboratory Standards standardized agar dilution MICs of ceftazidime, ciprofloxacin, piperacillin, and tobramycin for Pseudomonas aeruginosa during tests of 100 rough and mucoid P. aeruginosa isolates from cystic fibrosis patients. The levels of agreement (+/- 1 log2 dilution) between quantitative E-Test and agar dilution MIC results were 80, 97, 73, and 89% for ceftazidime, ciprofloxacin, piperacillin, and tobramycin, respectively. Comparison of the results after converting the MIC data to qualitative categories (susceptible, intermediate, and resistant) yielded levels of agreement of 84, 96, 88, and 93% for the same agents, respectively. Of the 39 qualitative discrepancies, 36 were minor and 3 were very major. We conclude that use of the E-Test is easier and more practical than use of the agar dilution method for most laboratories and that the E-Test furnishes results which are at least as accurate as those obtained by the agar dilution method. However, the higher cost of the E-Test method would likely discourage most laboratories from selecting it over disk diffusion for routine antimicrobial susceptibility testing of P. aeruginosa isolates from cystic fibrosis patients.


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  • Pitt, T L, Sparrow, M, Warner, M, Stefanidou, M (2003). Survey of resistance of Pseudomonas aeruginosa from UK patients with cystic fibrosis to six commonly prescribed antimicrobial agents. Thorax 58: 794-796 [Abstract] [Full Text]  
  • Saiman, L., Burns, J. L., Whittier, S., Krzewinski, J., Marshall, S. A., Jones, R. N. (1999). Evaluation of Reference Dilution Test Methods for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa Strains Isolated from Patients with Cystic Fibrosis. J. Clin. Microbiol. 37: 2987-2991 [Abstract] [Full Text]  
  • Di Bonaventura, G., Ricci, E., Della Loggia, N., Catamo, G., Piccolomini, R. (1998). Evaluation of the E Test for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa Isolates from Patients with Long-Term Bladder Catheterization. J. Clin. Microbiol. 36: 824-826 [Abstract] [Full Text]  


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