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Journal of Clinical Microbiology, Dec 1995, 3338-3340, Vol 33, No. 12
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Inhibitory effect of sodium dodecyl sulfate in detection of Mycobacterium tuberculosis by amplification of rRNA

JM Manterola, F Gamboa, J Lonca, L Matas, J Ruiz Manzano, C Rodrigo and V Ausina
Servicio de Microbiologia, Hospital Universitario Germans Trias i Pujol, Facultad de Medicina, Universidad Autonoma de Barcelona, Badalona, Spain.

The concentrations of sodium dodecyl (lauryl) sulfate (SDS) found in the sediments of the respiratory samples pretreated with SDS-NaOH varied between 3.36 and 12.42 mg/ml. These concentrations of SDS were higher than the level considered critical (> or = 0.16 mg/ml) to obtain negative results with the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. Extensive washing, after neutralization of sediment, is sufficient to remove all traces of detergent which interfere with the enzymes used by the Mycobacterium Tuberculosis Direct Test assay.


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  • Vincelette, J., Schirm, J., Bogard, M., Bourgault, A.-M., Luijt, D. S., Bianchi, A., van Voorst Vader, P. C., Butcher, A., Rosenstraus, M. (1999). Multicenter Evaluation of the Fully Automated COBAS AMPLICOR PCR Test for Detection of Chlamydia trachomatis in Urogenital Specimens. J. Clin. Microbiol. 37: 74-80 [Abstract] [Full Text]  
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